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RISS 인기검색어
- 검색키워드
- 저자 : Ramawat, Kishan G. (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Suthar, Satish,Ramawat, K.G. The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.1
Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the stimulatory effects of growth retardants [ALAR (N,N-dimethylaminosuccinamic acid) and CCC (chlormequat chloride)] and fungal elicitor on guggulsterone accumulation in cell cultures of C. wightii are reported. CCC at $1\;mg\;l^{-1}$ enhanced guggulsterone content (${\sim}123\;{\mu}g\;l^{-1})$ when added on the fifth day after inoculation, while ALAR at $2.5\;{\mu}g\;l^{-1}$ increased guggulsterone content (${\sim}116\;{\mu}g\;l^{-1}$) when added on the tenth day. In a two-stage fed-batch process, combined treatment with fungal elicitor and growth retardant caused a significant increase (${\sim}353\;{\mu}g\;l^{-1}$) in guggulsterone content in cell cultures after 17 days of growth. This represents an approximately fivefold increase over the guggulsterone contents in initial cultures of this plant.
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Chetana Roat,K. G. Ramawat 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.2
Cell cultures of Cayratia trifolia (Vitaceae), a tropical lianas, were maintained in Murashige and Skoog’s medium containing 0.25 mg l-1 NAA, 0.2 mg l-1 kinetin and casein hydrolysate 250 mg l-1. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin), which on elicitation by any of 500 lM salicylic acid, 100 lM methyl jasmonate, 500 lM ethrel and 500 mg l-1 yeast extract, added on the 7th day, were enhanced by 3- to 6-fold (5–11 mg l-1) by the 15th day. Cell cultures of Cayratia trifolia (Vitaceae), a tropical lianas, were maintained in Murashige and Skoog’s medium containing 0.25 mg l-1 NAA, 0.2 mg l-1 kinetin and casein hydrolysate 250 mg l-1. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin), which on elicitation by any of 500 lM salicylic acid, 100 lM methyl jasmonate, 500 lM ethrel and 500 mg l-1 yeast extract, added on the 7th day, were enhanced by 3- to 6-fold (5–11 mg l-1) by the 15th day.
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Goyal, Shaily,Sharma, Varsha,Ramawat, Kishan G. The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.2
Isoflavonoid production in cell cultures of Pueraria tuberosa as influenced by an angiospermic parasite, Cuscuta reflexa, was studied. During the time course, maximum isoflavonoid content was recorded when Cuscuta elicitor was added on day 15 of culture. Among various concentrations of elicitor tried, $1g\;l^{-1}$ of Cuscuta elicitor was found to be the most effective. The optimized elicitation conditions were used in vessels of varying capacity where maximum yield of ${\sim}91mg\;l^{-1}$ of isoflavonoid was recorded in a 2-l bioreactor which was about 19% higher than the control cultures. In this case, puerarin content increased up to $11mg\;l^{-1}$ which was 580% higher that the value recorded in the control cultures. In the bioreactor, 8 days of elicitation was optimal for the high accumulation of isoflavonoid, giving productivity of ${\sim}4mg\;l^{-1}\;day^{-1}$. The study showed persistent high isoflavonoid yield even during scale-up. Use of a preparation of Cuscuta reflexa as an elicitor is reported for the first time. The increase in isoflavonoid content was elicitor dose-dependent and can be explored to trigger high yields of isoflavonoid/secondary metabolites in production.
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Namira Arif,Nilesh C. Sharma,Vaishali Yadav,Naleeni Ramawat,Nawal Kishore Dubey,Durgesh Kumar Tripathi,Devendra Kumar Chauhan,Shivendra Sahi 한국식물학회 2019 Journal of Plant Biology Vol.62 No.4
Heavy metal (HMs) pollution is regarded as oneof the major concerns for soil and water, causing varieties oftoxic and stress effects on plants and ecosystems. It hasbecome one of the important limiting factors to cropproductivity and quality. Due to an ever-increasing populationgrowth and food demands, this situation has further worsened. Rice, a leading staple food crop that feeds more than 50%populations of the world, is constantly affected by abioticstressors including HMs. In most of the countries, a majorsource of HM intake by humans is the rice grain producedthrough the paddy soils contaminated with HMs such as As,Al, Cu, Cr, Cd, Pb, Hg, Mn, Se, and Zn. Thus, gradualagglomeration of HMs in rice grains and their subsequenttransfer to the food chain is a major menace to agricultureand human health. In recent years, several studies examinedthe impact of HMs toxicity on rice at multiple levels:molecular, biochemical, physiological, cellular and tissue,and demonstrated a correlation between HMs toxicity andthe decreasing trend in rice productivity. Therefore, it isnecessary to understand the interaction of HMs with ricecrop spanning from the cell to whole plant level and deviseappropriate effective means to alleviate these stress responses. This review focuses on tracing the pathways involved instress responses and stress tolerance mechanisms displayedby different varieties of rice. However, it is essential touncover the mechanisms related to stress responses in ricefor designing improved investigations to develop novelvarieties with high attributes. Therefore, this communication summarizes various defense strategies induced against HMstress and includes the function of metabolites (metabolomics),trace elements (ionomics), transcription factors (transcriptomics),and various stress-inducible proteins (proteomics) includingthe role of plant hormones.
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