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RISS 인기검색어
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- 저자 : Qingling Zhong (검색결과 3 건)
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alAn Intratracheal Challenge Murine Model of Asthma: Can Bronchial Inflammation Affect the Nose?
Jiaxing Xie,Yin Xi,Qingling Zhang,Guoqin Chen,Luo Wei,Kefang Lai,Nanshan Zhong 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.1
Purpose: Extensive data support the influence of the upper airway on lower airway inflammation and pathophysiology in allergic disease. However, few studies have focused on allergic inflammation in the nose after an isolated lower airway allergen challenge, a situation that can exist clinically when human subjects breathe primarily through the mouth, as occurs when nasally congested. This study used a mouse model to investigate whether upper airway inflammation and hyperresponsiveness were induced by an isolated lower airway allergen challenge. Methods: BALB/c mice were sensitized by systemic intraperitoneal injection of ovalbumin/saline and challenged with intratracheal ovalbumin/saline. Inflammation in the nose and lungs was assessed by cytology and histology of nasal tissues and bronchoalveolar lavage fluid (BALF), while nasal airway resistance and response were measured over 3 days post-challenge. Results: Intratracheal application of an allergen in anaesthetized mice resulted in exclusive deposition in the lower airway. Compared to control animals, ovalbumin -sensitized mice after challenge showed bronchial hyperreactivity and increased IL-5 in the serum BALF, as well as eosinophil infiltration in the lungs. However, nasal histology of the ovalbumin-sensitized mice showed no increase in eosinophil infiltration. The nasal lavage fluid revealed no increase in eosinophils or IL-5, and the nasal airway resistance did not increase after challenge either. Conclusions: In a mouse allergy model, exclusive allergen challenge of the lower airway can elicit a pulmonary and systemic allergic response, but does not induce upper airway inflammatory or physiological responses.
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Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801
Gong, Shasha,Meng, Qingling,Qiao, Jun,Huang, Yunfu,Zhong, Wenqiang,Zhang, Guowu,Zhang, Kai,Li, Ningxing,Shang, Yunxia,Li, Zhiyuan,Cai, Xuepeng The Korea Society for Parasitology and Tropical Me 2022 The Korean Journal of Parasitology Vol.60 No.5
Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)<sub>8</sub> triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40℃ and pH values between 4-7. Enzyme activity was inhibited by Zn2<sup>+</sup>, Ca2<sup>+</sup>, and Fe3<sup>+</sup>, whereas Mg2<sup>+</sup> and K<sup>+</sup> potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.
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Xiaoqi Tao,Qiqi Su,Hai Wu,Lei Zhan,Wei Huang,Jingshao Zheng,Qingling Zhong,Pei Chen 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.6
The investigation of the structure and physicochemical properties of starch extracted from a new variety of purple rice was the aim of this study. Starch extracted from a new variety of purple rice named Tianzi No. 1 (PRS) is different in structure and physicochemical properties compared with waxy rice starch (WRS), japonica rice starch (JRS), and indica rice starch (IRS). PRS granules were diversified in shape, and the birefringence of starch particles were clearly observed. Fourier-transform infrared (FT-IR) spectroscopy exhibited the degree of double helix and low short-range order structure of PRS differed from IRS, JRS and WRS. X-ray diffraction analysis shows that PRS presented a typical A-type XRD pattern and possessed lower crystallinity. Based on rheological experiment results, PRS had the highest apparent viscosity, storage modulus (G′) and loss modulus (G″). According to textural experiments, PRS gels had higher textural paraments before and after retrogradation.
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