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Faraz Arash Rad,Morad Jafari,Nabi Khezrinejad,Mahmoud Pouryousef Miandoab 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.6
An efficient and rapid plant regeneration system via direct organogenesis was established for felty germander(Teucrium polium L.), an endangered and valuable medicinal plant. Induction of shoot organogenesis was obtainedusing nodal explants and preconditioned intact seedlings on the MS medium supplemented with various concentrationsand combinations of plant growth regulators. The highest number of shoots (7.14/explants) was obtained from nodalexplants cultured on MS containing 2.0 mg·L-1 BAP and 0.5 mg·L-1 NAA. For preconditioning, Best response in termsof number of adventitious shoots per explant (6.85) and greatest shoot length (5.43 cm) was obtained with seedlingsperpetrated in 0.5 mg·L-1 TDZ. The rooted plantlets were acclimatized successfully in the greenhouse with a survivalrate of 100%. Significant differences (p < 0.05) were found between the greenhouse-grown tissue culture raised plantsin total flavonoid contents. Methanolic extracts of plants raised on TDZ (0.5 mg·L-1) containing medium showed thehighest amount of flavonoid contents (1.67 mg Ru·g-1 DW) and a higher antioxidant activity (52.31%) in comparisonwith the field-grown mother plant. Randomly amplified polymorphic DNA (RAPD) analysis of plants raised preconditioningprocedure revealed there were no somaclonal variations among them. Our results indicated that thecytokinin-preconditioning mediated micropropagation protocol could be used for the production of a large number oftrue-to-type plants of T. polium, which may contain higher quantities of valuable phytochemicals than those of wildplants.