Methods of Mineral Engineering in a Fight Against Varrosa Infestation

Maciej Pawlikowski,Hubert Przybyszewski,Lech Stę,pień, 한국식품보건융합학회 2019 식품보건융합연구 (KJFHC) Vol.5 No.1

The purpose of previous study centered around introducing minerals into bee colonies was to observe the Varrosa destructor mites-repelling effect of minerals (“Pszczelarstwo” 6/2016). The results of research published so far confirmed the purpose of using minerals in the fight against Varrosa infestation. This publication presents partial results of the next stage of research. Using foundation enriched with studied minerals in right proportions leads to diminishing the number of Varroa destructor parasites in bee colonies. It may also contribute to increase in the amount of honey obtained from bees. Foundations with minerals were in most cases correctly filled with larvae, and the new generation of bees didn’t show any morphometric or physical-motor aberrations. The effect of minerals in the foundation weakens with time, which requires adequate reaction from the beekeeper. The study showed no negative effect of mineral suspensions added directly to royal jelly of larvae in the swarm cells and worker cells on the development of bees. Additional research was carried out in August and September and led to surprising observations, which are still too early to report. It obliges us to carry out further, extended subject research on a large number of bee colonies in 2017.

Tissue-Specific Expression of Nitric Oxide Synthase in Nutrient Digestive Organs

Pie, Jae-Eun 안양대학교 산업기술연구소 2001 自然科學硏究 Vol.8 No.-

Using the human samples of trauma patients (IRB approved), differential expression levels of mRNAs and proteins of nitric oxide synthase (NOS) were determined in several digestive organs, including small and large intestines. Two different sizes (^~11 kb in testis, small intestine and colon, and ^~12 kb in other tissues examined) of nNOS mRNA were detected in tissue specific patterns. In the Western-blot analysis, however, at least three different nNOS bands (130, 140, and 160 kDa) were observed, particularly in small intestine and colon (160 and 140 kDa). And two iNOS mRNAs of different sizes (4.5 and 4.2 kb) were also expressed constitutively at different levels in peripheral tissues including small intestine and colon. In some tissues, however, only a single band iNOs protein was observed. As with the iNOS mRNAs, two eNOS mRNAs of different sizes (4.4 and 4.2 kb) were detected in almost all tissues examined. These tissue-specific or differential expression of NOS mRNAs and proteins are produced by alternative splicing and this may be associated with the diverse tissue specific roles of NO observed in vivo, especially in digestive organs.

Nitric Oxide Dependency in Inflammatory Response-related Gene Transcripts Expressed in Lipopolysaccharide-treated RAW 264.7 Cells

Pie, Jae-Eun,Yi, Hyeon-Gyu The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.4

Cytotoxic Nitric oxide (NO) overproduced by inducible NO Synthase (iNOS or NOS2), which was induced in inflammatory reactions and immune responses directly or indirectly affects the functions as host defense and can cause normal tissue damage. Microarray analysis was performed to identify gene profiles of both NO-dependent and -independent transcripts in RAW 264.7 macrophages that use selective NOS2 inhibitors aminoguanidine ($100\;{\mu}M$) and L-canavanine (1 mM). A total of 3,297 genes were identified that were up- or down-regulated significantly over 2-fold in lipopolysaccharide (LPS)-treated macrophages. NO-dependency was determined in the expressed total gene profiles and also within inflammatory conditions-related functional categories. Out of all the gene profiles, 1711 genes affected NO-dependently and -independently in 567 genes. In the categories of inflammatory conditions, transcripts of 16 genes (Pomp, C8a, Ifih1, Irak1, Txnrd1, Ptafr, Scube1, Cd8a, Gpx4, Ltb, Fasl, Igk-V21-9, Vac14, Mbl1, C1r and Tlr6) and 29 geneas (IL-1beta, Mpa2l, IFN activated genes and Chemokine ligands) affected NO-dependently and -independently, respectively. This NO dependency can be applied to inflammatory reaction-related functional classifications, such as cell migration, chemotaxis, cytokine, Jak/STAT signaling pathway, and MAPK signaling pathway. Our results suggest that LPS-induced gene transcripts in inflammation or infection can be classified into physiological and toxic effects by their dependency on the NOS2-mediated NO release.

Positive Correlation between Suppressed Brug Metabolizing Enzymes and Increased iNOS Expression in Mouse Liver after Schistosoma japonicum Infection

Pie, Jae-Eun 안양대학교 산업기술연구소 2000 自然科學硏究 Vol.7 No.-

Activities of two major hepatic drug metabolizing enzymes, cytochrome P450(CYP) and flavin-containing monooxygenase (FMO) were examined in mice after a moderate infections of S. japonicum. Liver microsomal CYP activities determined by testosterone hydroxylation and caffeine oxidation and FMO activities analyzed by ranitidine N-oxidation were significantly decreased by 6 weeks but recovered in 9 weeks with significant difference between male and female. Conversely. the hepatic iNOS mRNA contents increased maximally at 6-7 weeks but decreased toward normal undetectable level by 9 weeks. Immunohistochemical detection of iNOS expression sites showed initial moderate staining at Kupffer cells, intense staining of granuloma cells forming a halo surrounding the deposited eggs (6-7 weeks) and dark focal staining only in the core of dying parasite eggs (9 weeks). Results suggested that the NO produced by iNOS and the super-oxide anion (02-) produced by the granulomatous cells may generate the toxic peroxynitrite (ONOO-). This, in turn, may mediate the suppression of the drug metabolism by inhibiting the CYP and FMO activities in hepatocytes.

The Studies on the Impact of Tailored DCA Treated Ethanol Blends on Engine Emissions

Stępień Zbigniew,Urzędowska Wiesŀawa 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6

The basic criterion in using alternative fuels, specifically ethanol blends, consists in its pro-ecological properties. However, like in the case of conventional hydrocarbon fuels, the use of blended ethanol in combustion engines can result in formation of harmful engine deposits. The addition of DCA (Deposit Control Additive) type fuel improvers is the most effective approach to prevent harmful deposit formation. However, such additives, although widely used in engine petrol types, are usually not fully compatible and hence effective in the case of ethanol fuels. Because of this, an effective DCA was developed under the carried out project, dedicated to ethanol fuels. The project was aimed at carrying out a comparative assessment of the impact of the developed DCA additive to ethanol fuels on engine emission and fuel consumption. Using a chassis dynamometer test with exhaust gas analyzers and Fourier-transform infrared spectroscopy, regulated emissions of CO, NMHC, HC, THC, NOx and PM, PN were investigated, as well as CO2, and unregulated emissions of CH4, HCHO, MeCHO, NH3, ETOH. This allowed evaluating, whether the use in ethanol fuels of a new DCA, of effectiveness confirmed in tests, creates a threat in the field of increased emission of a combustion engine supplied with so improved fuel.

Correlation between nutrition intake and gene expression profiles in children with asthma

Pie, Jae-Eun,Kim, Yu-Ri,Kim, In-Kyoung,Seo, Sang-Hui,Lee, Seung-Ho,Lee, Hee-Ra,Yoo, Young,Chung, Ji-Tae,Youn, Jong-Pil,Oh, Moon-Ju,Hwang, Seung-Yong,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2010 Molecular & cellular toxicology Vol.6 No.3

Asthma is a complex inflammatory disease and its prevalence has increased worldwide, especially in young children. In this study, we used a '24-hour recall method' to identify differences between children with and without asthma in energy intake and energy-adjusted nutrition intake. We also performed reverse transcription-polymerase chain reaction (RT-PCR) with pathway-targeted arrays (RT2 $Profiler^{TM}$ PCR Array) to investigate the expression profiles of chemokines and cytokines in children with asthma. The intake of vitamin C in mild and moderate asthma was significantly higher than that in healthy controls. Vitamin E intake in the mild asthma group was also significantly higher. Twenty-three genes were expressed at higher levels in severe asthma compared with healthy controls. Using the human Th1-Th2-Th3 PCR Array, we found 17 genes were upregulated in severe asthma, including the Th2-related genes CCL7, IL13, and CCL-11 (eotaxin). These PCR Array results revealed that the genes that were most profoundly increased in asthma encoded for key proinflammatory and chemotactic molecules. Our observations lead us to speculate that the interaction between gene expression and dietary intake is important for the development of asthma.

Wireless Measurement of Carbon Monoxide Concentration

Martin Pies,Radovan Hajovsky,Stepan Ozana 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10

The paper deals with measurement of concentration of highly toxic carbon monoxide within the space of waste rock storage. This purpose gave origin to creation of units with integrated sensors of carbon monoxide that transmit measured concentration by means of wireless IQRF technology. The need of decreased power consumption implicated choice of electrochemical CO sensor connected to a special wireless technology named IQRF, working at 868 MHz frequency. Concentration is measured at two locations within waste rock dump. The paper analyzes consumption of energy spent on this type of measurement and it evaluates assumed endurance for battery-operated mode. Concentration of CO is sent to IQRF-AO converter that transfer the measured value to voltage level by means of double-channel DAC converter. This voltage is then brought to the inputs of telemetry unit that transmits the values of CO and other measured signals to a supervisor dispatching center.

HOW RETAILERS CONTRIBUTE TO SUSTAINABILITY PROMOTION IN THE LUXURY FASHION SECTOR?

Beata Stępień 글로벌지식마케팅경영학회 2019 Global Fashion Management Conference Vol.2019 No.07

단백질의 종류가 체내 칼슘대사에 미치는 영향에 관한 연구

피재은(Pie Jae-Eun),백희영(Paik Hee Young) 韓國營養學會 1986 Journal of Nutrition and Health Vol.19 No.1

Digital Holographic Microscopy with extended field of view using tool for generic image stitching

Piotr Stępień,Damian Korbuszewski,Małgorzata Kujawińska 한국전자통신연구원 2019 ETRI Journal Vol.41 No.1

This paper describes in detail the processing path leading to successful phase images stitching in digital holographic microscope for the extension of the field of view. It applies FIJI Grid/Collection Stitching Plugin, which is a general tool for images stitching, non‐specific for phase images. The FIJI plugin is extensively supported by aberration and phase offset correction. Comparative analysis of different aberration correction methods and data processing strategies is presented, together with the critical analysis of their applicability. The proposed processing path provides good background for statistical phase analysis of cell cultures and digital phase pathology.