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( Jiang Li ),( Yu Jie Sha ),( Dewi Seswita Zilda ),( Qiu Shi Hu ),( Pei Qing He ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
An extracellular agarase was purified from Bacillus sp. BI-3, a thermophilic agar-degrading bacterium isolated from a hot spring in Indonesia. The purified agarase revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular mass of 58 kDa. The optimum pH and temperature of the agarase were 6.4 and 70oC, respectively. The activity of the agarase was stable at high temperatures, and more than 50% activity was retained at 80oC for 15 min. Furthermore, the enzyme was stable in the pH range of 5.8?8.0, and more than 60% of the residual activity was retained. Significant activation of the agarase was observed in the presence of K+, Na+, Ca2+, Mg2+, and Sr2+; on the other hand, Ba2+, Zn2+, Cu2+, Mn2+, Co2+, Fe2+, and EDTA inhibited or inactivated the enzyme activity. The components of the hydrolytic product analyzed by thin-layer chromatography showed that the agarase mainly produced neoagarobiose. This study is the first to present evidence of agarolytic activity in aerobic thermophilic bacteria.
Cho-Pei Jiang,Yo-Yu Chen 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.
Most bone scaffolds made of single biomaterial resulting in limited compressive strength and single property of cell adhesive. In thisstudy, sandwich structures of the bone scaffold with different biomaterials are fabricated to mimic the mechanical properties of humanbone. The fabricated scaffolds are also evaluated the cellular efficacy by cell culturing. In this study, biopolymer (mPEG-PCL-mPEG)is synthesized. Biocomposite is made of blending biopolymer with nano-hydroxyapatite (nHA). Both biomaterials are used to form thebone scaffolds with average pore size of 300μm using the developed dual-nozzle bioplotter system. Hybrid scaffolds with sandwichstructures are proposed and fabricated. All scaffolds are underwent the compressive and microhardness test for comparing themechanical properties with human bone. Furthermore, a mouse pre-osteoblast MC3T3-E1cell line is seeded onto fabricated scaffoldsand cultured for 28 days for validating the biocompatibility and evaluating the cellular efficacy using methylthiazol tetrazolium (MTT)assay. Biocomposite scaffold has the highest ultimate compressive strength and microhardenss among the others. Ultimatecompressive strength and microhardness of fabricated scaffold in vertical build plane are higher than those in horizontal build plane. Sandwich architecture of hybrid bone scaffold with biocomposite outer layer and biopolymer inner layer can improve cell adhesiveand cellular efficacy.
( Kai Zhi Xie ),( Pei Zhi Xu ),( Shao Hai Yang ),( Yu Sheng Lu ),( Rui Ping Jiang ),( Wen Jie Gu ),( Wen Ying Li ),( Li Li Sun ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5
Cold water paddy field soils are relatively unproductive, but can be ameliorated by supplementing with inorganic fertilizer from animal waste-based composts. The yield of two rice cultivars was significantly raised by providing either chicken manure or cow dung-based compost. The application of these composts raised the soil pH as well as both the total nitrogen and ammonium nitrogen content, which improved the soil’s fertility and raised its nitrification potential. The composts had a measurable effect on the abundance of nitrogencycling- related soil microbes, as measured by estimating the copy number of various bacterial and archaeal genes using quantitative real-time PCR. The abundance of ammonia oxidizing archaea and bacteria was markedly encouraged by the application of chicken manure-based compost. Supplementation with the composts helped promote the availability of soil nitrogen in the cold water paddy field, thereby improving the soil’s productivity and increasing the yield of the rice crop.
( Chien Hui Syu ),( Pei Yu Jiang ),( Hsuan Han Huang ),( Wen Ting Chen ),( Tzu Huei Lin ),( Dar Yuan Lee ) 한국환경농학회 2012 한국환경농학회 워크샵자료 Vol.2012 No.2
The iron plaque formed on rice root has been confirmed to be a barrier on the uptake of arsenic in many hydroponic experiments. However, few studies provide the information about the relationship between soil characteristics, iron plaque formation and As uptake by rice in Ascontaminated soils. Therefore, the aim of this study is to investigate the effect of the rice root`s iron plaques on the As uptake by rice plants grown in geologically As-contaminated soils with high contents of iron oxides and organic matter from the Guandu Plain of northern Taiwan. A soil flooding incubation study was performed and a pot experiment was conducted. The rice seedling was pre-cultivated in solution cultures and then transplanted in three level As-contaminated soils for growing 39 days. The amounts of iron plaque on rice root were determined by extraction using dithionite-citrate-bicarbonate (DCB). The As species on iron plaque and the concentrations of As and Fe in iron plaque, root, root base and shoot of rice plants were determined. The results of the soil incubation study showed that both As and Fe concentrations in the soil solutions increased with flooding time due to reductive dissolution of iron oxides induced by high contents of organic matter in soils. High amounts of iron plaque were deposited on rice roots and large amounts of As were sequestrated in these iron plaques. Results of the As K-edge X-ray absorption nearedge spectroscopy (XANES) indicated that arsenate was the main species of arsenic sorbed on iron plaque of rice roots. About 74.0 to 93.2 % of total As released from soils were distributed in the iron plaques and only small proportion was distributed in the rice plants. This study provides evidence that the iron plaques of the rice roots was the main controlling factor in limiting the uptake and accumulation of As into the rice plants grown in paddy soils with high contents of iron oxides and organic matter, meanwhile, it suggests that enhancement of iron plaque formation could be the approach used for reducing the uptake of As by paddy rice grown in As-contaminated soils.
He, Ying-Xia,Ye, Cheng-Lin,Zhang, Pei,Li, Qiao,Park, Chae Gyu,Yang, Kun,Jiang, Ling-Yu,Lv, Yin,Ying, Xiao-Ling,Ding, Hong-Hui,Huang, Hong-Ping,Mambwe Tembo, John,Li, An-Yi,Cheng, Bing,Zhang, Shu-Sheng American Society for Microbiology 2019 Infection and immunity Vol.87 No.1
<P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals.</P><P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer’s patches to initiate dissemination. In this study, we demonstrate that <I>Y. pseudotuberculosis</I> utilizes its lipopolysaccharide (LPS) core to interact with CD209 receptors, leading to invasion of human dendritic cells (DCs) and murine macrophages. These <I>Y. pseudotuberculosis</I>-CD209 interactions result in bacterial dissemination to MLNs, spleens, and livers of both wild-type and Peyer’s patch-deficient mice. The blocking of the <I>Y. pseudotuberculosis</I>-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity. Based on the well-documented studies in which HIV-CD209 interaction leads to viral dissemination, we therefore propose an infection route for <I>Y. pseudotuberculosis</I> where this pathogen, after penetrating the intestinal mucosal membrane, hijacks the <I>Y. pseudotuberculosis</I>-CD209 interaction antigen-presenting cells to reach their target destinations, MLNs, spleens, and livers.</P>
Analysis of Cancer Incidence in Zhejiang Cancer Registry in China during 2000 to 2009
Du, Ling-Bin,Li, Hui-Zhang,Wang, Xiang-Hui,Zhu, Chen,Liu, Qing-Min,Li, Qi-Long,Li, Xue-Qin,Shen, Yong-Zhou,Zhang, Xin-Pei,Ying, Jiang-Wei,Yu, Chuan-Ding,Mao, Wei-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14
Objective: The Zhejiang Provincial Cancer Prevention and Control Office collected cancer registration data during 2000 to 2009 from 6 cancer registries in Zhejiang province of China in order to analyze the cancer incidence. Methods: Descriptive analysis included cancer incidence stratified by sex, age and cancer site group. The proportions and cumulative rates of 10 common cancers in different groups were also calculated. Chinese population census in 1982 and Segi's population were used for calculating age-standardized incidence rates. The log-linear model was used for fitting to calculate the incidence trends. Results: The 6 cancer registries in Zhejiang province in China covered a total of 60,087,888 person-years during 2000 to 2009 (males 30,445,904, females 29,641,984). The total number of new cancer cases were 163,104 (males 92,982, females 70,122). The morphology verified cases accounted for 69.7%, and the new cases verified only by information from death certification accounted for 1.23%. The crude incidence rate in Zhejiang cancer registration areas was $271.5/10^5$ during 2000 to 2009 (male $305.41/10^5$, female $236.58/10^5$), age-standardized incidence rates by Chinese standard population (ASIRC) and by world standard population (ASIRW) were $147.1/10^5$ and $188.2/10^5$, the cumulative incidence rate (aged from 0 to 74) being 21.7%. The crude incidence rate was $209.6/10^5$ in 2000, and it increased to $320.20/10^5$ in 2009 (52.8%), with an annual percent change (APC) of 4.51% (95% confidence interval, 3.25%-5.79%). Age-specific incidence rate of 80-84 age group was achieved at the highest point of the incidence curve. Overall with different age groups, the cancer incidences differed, the incidence of liver cancer being highest in 15-44 age group in males; the incidence of breast cancer was the highest in 15-64 age group in females; the incidences of lung cancer were the highest in both males and females over the age of 65 years. Conclusions: Lung cancer, digestive system malignancies and breast cancer are the most common cancers in Zhejiang province in China requiring an especial focus. The incidences of thyroid cancer, prostate cancer, cervical cancer and lymphoma have increased rapidly. Prevention and control measures should be implemented for these cancers.