Evaluation of in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius and Trianthema decandra

Upal Kanti Mazumder,Malaya Gupta,Chandi Charan Kandar,Pallab Kanti Haldar 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3

Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and Fe2+–ascorbate induced by lipid peroxidation assay. The IC50 values of the both extracts were calculated from the inhibition curve. The IC50 MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 µg/ml respectively. Both the extracts exhibited a significant antioxidant effects.

Evaluation of in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius and Trianthema decandra

Mazumder, Upal Kanti,Gupta, Malaya,Haldar, Pallab Kanti,Kandar, Chandi Charan Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3

Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and $Fe^{2+}$-ascorbate induced by lipid peroxidation assay. The $IC_{50}$ values of the both extracts were calculated from the inhibition curve. The $IC_{50}$ MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 ${\mu}g/ml$ respectively. Both the extracts exhibited a significant antioxidant effects.

Preclinical evaluation of Kali Haldi (Curcuma caesia): a promising herb to treat type-2 diabetes

Poulami Majumder,Pallab Kanti Haldar,Suchandra Mazumder,Mainak Chakraborty,Srila Ghosh Chowdhury,Samit Karmakar 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.2

The methanol extract of Curcuma caesia (MECC) rhizome showed antidiabetic activity by lowering blood glucose in in vivo studies and controlled intestinal absorption of monosaccharides by inhibiting alpha amylase and alpha glucosidase. It also enhanced the glucose uptake in yeast cells, proving proper glucose utilization. The IC50 values of MECC were found to be 442.92 ± 10.05 μg/ml and 95.40 ± 9.74 μg/ml in alpha amylase and alpha glucosidase inhibition respectively. The extract has effective antioxidant activities, by successfully scavenging free radicals like superoxide and hydroxyl ions. The histopathological studies of pancreas showed gradual healing after treatment.

Gastrointestinal effects of triterpenoid enriched extract of Trichosanthes dioica root in albino mice

Bhattacharya, Sanjib,Haldar, Pallab Kanti 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.2

Trichosanthes dioica Roxb. (Cucurbitaceae), is a dioecious climber, traditionally used in India for several medicinal purposes including purgative. The present study aimed to evaluate the gastrointestinal effects (laxative and prokinetic) of triterpenoid enriched extract of T. dioica root (CETD) in Swiss albino mice. The laxative activity of CETD (50 and 100 mg/kg body weight per os) was evaluated by assessing the different excretory bowel activities in naive (non-constipated) and in drug (loperamide)-induced constipation in mice. Further, the gastrointestinal transit was measured in both naive and in constipated mice to ascertain the prokinetic effect. Castor oil (0.5 ml/mouse per os) was used as reference drug. CETD significantly and dose dependently increased all excretory bowel activities and gastrointestinal transit in both naive and in constipated mice. CETD at 100 mg/kg body weight was found to be the most active causing diarrhoea in mice. Therefore, T. dioica root extract demonstrated remarkable stimulant laxative and prokinetic effects in Swiss albino mice validating its prime ethnomedicinal usage in the Indian traditional medicine.

CNS depressant activity of Castanopsis indica leaves

Dolai, Narayan,Karmakar, I.,Kumar, R.B.S.,Haldar, Pallab Kanti 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.2

Castanopsis indica (Roxb. ex Lindl.) A.DC. (Fagaceae) is commonly known as 'Dhalne katus' and 'Indian chestnut tree', found throughout the Himalayan region. The purpose of this investigation was to evaluate the effect of methanol extract of C. indica leaves (MECI) on Central Nervous System using a number of neuropharmacological experimental models in Swiss albino mice. The treatment with MECI at doses of 25 and 50 mg/kg, i.p. caused: signs of central nervous system depressant action confirmed by significant inhibitory effect on acetic acid induced writhing as compared with acetic acid control group indicate an analgesic effect. Anticonvulsant activity was seen as there was delays in the onset of pentylenetetrazole (PTZ) induce convulsion as well as decrease in its mortality rate. The MECI produced a dose-dependent reduction of the onset and potentiated duration of pentobarbitone-induced hypnosis, reduction of locomotor and muscle relaxant activities determining by using actophotometer and rotarod tests. MECI in a dose-dependent manner significantly reduced the immobility time of mice in both forced swim test (FST) and tail suspension test (TST). These results suggest that the MECI has an analgesic effect, behave as a potential anticonvulsant and central nervous system depressant action.

Protective effect of Basella alba leaf against diabetic nephropathy in rats

Kumari Reshma,Goldar Waquar Ahmed,Mondal Subhodeep,Patra Susmita,Bhattacharya Sanjib,Haldar Pallab Kanti 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.1

Chronic hyperglycemic condition leads to development of diabetic nephropathy (DN). Basella alba is an important edible vegetable in India. Present study was conducted to inquire the effects of methanol extract from Basella alba leaf (MEBA) against diabetic nephropathy in rats. A single intraperitoneal injection of streptozotocin (55 mg/kg) was given to Wistar albino rats to induce diabetes. After 4 weeks of diabetes induction, the rats were divided into normal control group, disease DN group, MEBA treatment groups (200 and 400 mg/kg), metformin treatment group (150 mg/kg) and the treatment was continued for next 3 weeks. In MEBA treated groups there were marked improvements in blood glucose, ALP, ALT, total pro-tein, serum lipid profile such as total cholesterol, triglyceride, HDL levels and renal functions such as urine volume, urinary protein excretion, serum creatinine, blood urea nitrogen, creatinine clearance as compared to non-treated DN group. There was significant rise in malonaldehyde level, and decline in acivites of superoxide dismutase and reduced Glutathione (GSH) of kidney in diabetic rats as compared to normal control group rats. The MEBA treated groups showed dose dependent resto-ration of altered oxidative stress parameters. Moreover, from renal histopathology it was evident that the abnormal thickening of Glomerular basement membrane in kidney of diabetic rats were improved in MEBA treated rats. From the present study it can be concluded that, Basella alba leaf has marked protective effect against diabetic nephropathy and proteinuria in rats.

Scavenging activity of $Curcuma$ $caesia$ rhizome against reactive oxygen and nitrogen species

Karmakar, Indrajit,Dolai, Narayan,Saha, Pathik,Sarkar, Nilanjan,Bala, Asis,Haldar, Pallab Kanti 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.4

$Curcuma$ $caesia$ Roxb. (Zingiberaceae), known as black turmeric in English, is a perennial herb found throughout the Himalayan region, North-East and Central India. The plant has been traditionally used in India for several medicinal purposes. Present study was carried out to evaluate the methanol extract of $C.$ $caesia$ (MECC) rhizome for some $in$ $vitro$ antioxidant studies as because we know that many diseases are associated with reactive oxygen species (ROS) and reactive nitrogen species (RNS). Effect of MECC on ROS and RNS were evaluated in different $in$ $vitro$ methods like 1, 1-diphenyl-2-picrylhydrazil radical, hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite and hypochlorous acid. Lipid peroxidation, total phenolic content was also measured by standard assay method. The extract showed significant antioxidant activities in a dose dependent manner. The $IC_{50}$ values for scavenging of free radicals were $94.03{\pm}0.67{\mu}g/ml$, $155.59{\pm}3.03{\mu}g/ml$, $68.10{\pm}1.24{\mu}g/ml$, $21.07{\pm}1.78{\mu}g/ml$, $260.56{\pm}12.65{\mu}g/ml$ and $33.33{\pm}0.52{\mu}g/ml$ for DPPH, nitric oxide, superoxide, hydroxyl, peroxynitrite and hypochlorous acid respectively. Reductive ability of the extract was also tested where dose dependent reducing capability was observed. The rhizome extract contains 677.7 ${\mu}g$ of phenolic compound in 10 mg of the extract which is accounted for its free radical as well as antioxidant activity. From the above study it is concluded that the methanol extract of $C.$ $caesia$ rhizome is a potential source of natural antioxidant.

Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species

Indrajit Karmakar,Narayan Dolai,Pathik Saha,Nilanjan Sarkar,Asis Bala,Pallab Kanti Haldar 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.4

Curcuma caesia Roxb. (Zingiberaceae), known as black turmeric in English, is a perennial herb found throughout the Himalayan region, North-East and Central India. The plant has been traditionally used in India for several medicinal purposes. Present study was carried out to evaluate the methanol extract of C. caesia (MECC) rhizome for some in vitro antioxidant studies as because we know that many diseases are associated with reactive oxygen species (ROS) and reactive nitrogen species (RNS). Effect of MECC on ROS and RNS were evaluated in different in vitro methods like 1, 1-diphenyl-2-picrylhydrazil radical, hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide,peroxynitrite and hypochlorous acid. Lipid peroxidation,total phenolic content was also measured by standard assay method. The extract showed significant antioxidant activities in a dose dependent manner. The IC_(50) values for scavenging of free radicals were 94.03±0.67 μg/ml, 155.59±3.03 μg/ml,68.10±1.24 μg/ml, 21.07±1.78 μg/ml, 260.56±12.65 μg/ml and 33.33±0.52 μg/ml for DPPH, nitric oxide, superoxide,hydroxyl, peroxynitrite and hypochlorous acid respectively. Reductive ability of the extract was also tested where dose dependent reducing capability was observed. The rhizome extract contains 677.7 μg of phenolic compound in 10 mg of the extract which is accounted for its free radical as well as antioxidant activity. From the above study it is concluded that the methanol extract of C. caesia rhizome is a potential source of natural antioxidant.