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Development of insecticide resistance molecular maker and their application
Juil Kim,Min Kwon,Nicole Joussen,David G. Heckel 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
Worldwide, increasing numbers insecticide resistant insect is one of the main problem in this agricultural era. To manage the insecticide resistance, IRAC suggest some tips for farmers : 1. Sequence or alteration of insecticide based on MOA 2. Choosing the effective insecticide. In this point raised following question : How to distinguish the effective or non-effective insecticide. Until now, bioassay is the right answer. However that is a very time-consuming, labor-intensive, and costly process, so we were hoping that molecular based diagnosis could be alternate. It can summarized following steps : 1. Identification of insecticide resistance mechanism 2. Marker development 3. Molecular diagnosis 4. Selection of non-effective insecticide. Here we focused on the development of insecticide resistance marker. Point mutation in target site of insecticide is a good example. Up or down-regulation of specific gene and/or existence of chimeric gene is another example from Helicoverpa armigera.