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RISS 인기검색어
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- 저자 : Nian Min Qi (검색결과 5 건)
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Weiwei Li,Min Li,Yantian Chen,Shaoming Zhang,Nian Min Qi 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
Mesenchymal stem cells derived from humanumbilical cords (hUCMSCs) are attractive as a new cellsource for tissue engineering. It is essential to investigateand optimize the seeding process of these cells for thesuccess of cell culture and tissue regeneration in vitro. In thisstudy, a static seeding method (SSM), a centrifugal seedingmethod (CSM), and a novel method-cycling filtrationseeding method (CFSM) are evaluated in terms of seedingefficiency, cell damage, and distribution inside the scaffolds,cell proliferation, and osteogenic differentiation. Cells wereseeded on three-dimensional (3-D) nonwoven PET discs ata density of 1×104 cells/disc, followed by 21 days of cellculture and 20 days of osteogenic differentiation. Cellsgrown in 3-D conditions exhibited higher metabolic activitythan those grown on a 2-D control surface. The CSM andCFSM groups showed higher seeding efficiency, proliferationcapacity, and differentiation potential. H&E staining indicateda more uniform spatial distribution of cells in CFSMgroups. LDH level measurements suggested that more celldamage was caused by the CFSM process. Above all, theresults showed that the cells maintained their proliferationability and differentiation potential ex vivo during approximately7 weeks of culture. The CSM and CFSM arerecommended for hUCMSC tissue engineering, althoughthe seeding parameters still require further investigationand optimization.
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Gui Rong Wang,Nian Min Qi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6
Plant adventitious root culture in bioreactors is a promising alternative for the efficient production of medicinal herbs. Adventitious roots of Pseudostellaria heterophylla were induced from callus and then cultivated in a siphon-mist bioreactor. An orthogonal test established that the optimal medium for adventitious root induction was MS medium supplemented with 1.0 mg/L naphthaleneacetic acid and 2.0 mg/L 3-indolybutyric acid. Under these conditions, the average root number was more than 14 on each 1.0 cm diameter callus and the rooting rate reached 100%. The bioreactor was equipped with an integral siphon-spraying device designed to automatically supply the liquid medium. The operation parameters of the bioreactor were assessed by varying the mist interval and the aeration velocity. The mist interval was negatively related to average growth rate of the adventitious roots and positively related to saponin and polysaccharide content. A relatively high aeration rate was necessary to achieve the maximum biomass production, but the secondary metabolite production was not enhanced by increasing the aeration velocity.
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HIF-1α and GLUT1 Gene Expression is Associated with Chemoresistance of Acute Myeloid Leukemia
Song, Kui,Li, Min,Xu, Xiao-Jun,Xuan, Li,Huang, Gui-Nian,Song, Xiao-Ling,Liu, Qi-Fa Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4
Aims: Much evidence suggests that increased glucose metabolism in tumor cells might contribute to the development of acquired chemoresistance. However, the molecular mechanisms are not fully clear. Therefore, we investigated a possible correlation of mRNA expression of HIF-$1{\alpha}$ and GLUT1 with chemoresistance in acute myeloid leukemia (AML). Methods: Bone marrow samples were obtained from newly diagnosed and relapsed AML (M3 exclusion) cases. RNA interference with short hairpin RNA (shRNA) was used to stably silence GLUT1 or HIF-$1{\alpha}$ gene expression in an AML cell line and HIF-$1{\alpha}$ and GLUT1 mRNA expression was measured by real-time quantitative polymerase chain reaction assay (qPCR). Results: High levels of HIF-$1{\alpha}$ and GLUT1 were associated with poor responsiveness to chemotherapy in AML. Down-regulation of the expression of GLUT1 by RNA interference obviously sensitized drug-resistant HL-60/ADR cells to adriamycin (ADR) in vitro, comparable with RNA interference for the HIF-$1{\alpha}$ gene. Conclusions: Our data revealed that over-expression of HIF-$1{\alpha}$ and GLUT1 might play a role in the chemoresistance of AML. GLUT1 might be a potential target to reverse such drug resistance.
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Liu Yang,Hai-Ying Sun,Nian-Min Qi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2
Applications of bioreactors in combination with scaffolding materials are promising in tissue-engineering fields. To rapidly produce bone mesenchymal stem cells (MSCs) suitable for osteogenic differentiation (OSD), we developed a novel technique for β-TCP (β-tricalcium phosphate)scaffolds preparation and employed these scaffolds to build a new type of mini three dimensional (3D) perfusion bioreactor. Compared to the 2D static culture, MSCs acquired much higher amplification rate and alkaline phosphatase (ALP) activity over a 24-day culture period. Interestingly, the Specific ALP activity was independent of the growth rate under adequate osteogenic inducement,suggesting there may be an OSD bottleneck at a single-cell level. Furthermore, cells on scaffolds exhibited gradually reduced total migration rate (MR) and relatively constant local MR, both ideal for bone regeneration. Excellent adhesion of MSCs to the smooth scaffolds surface, especially the layer structures, was seen on scanning electron microscopy images, demonstrating fine compatibility between scaffold and cells. Our results indicate this simplified,integrated and potentially modularizable 3D bioreactor could enable the osteocytes producing from MSCs for expected applications.
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Liu Yang,Hai-Ying Sun,Nian Min Qi,Wen-Song Tan 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
To readily supply seed cells for tissue engineering and ensure their constant availability for experiments,it is imperative to establish an in-situ cryopreservation method for cell storage. We investigated the effects of a β-tricalcium phosphate (β-TCP) 3D scaffold in-situ cryopreservation method on the migration rate and osteogenic ability of mesenchymal stem cells (MSCs). Compared to using a 2D plate culture and trypsinized cryopreservation, MSCs on β-TCP 3D scaffolds demonstrated a higher amplification rate, and the harvest and survival rates (HSR) increased from 55.9 to 81.3% when the 3D in-situ cryopreservation method was applied. The cell migration rate and alkaline phosphatase (ALP) activity were unaffected after in-situ cryopreservation, and unexpectedly,the Specific ALP activity of migrating cells was higher than that of non-cryopreserved cells, suggesting that the cell-scaffold combination could be cryopreserved using the present protocol without loss of proliferative or osteogenic potential. These findings highlight a methodology for 3D scaffold in-situ cryopreservation and passage for MSC production in bone tissue engineering, and present the possibility of designing a perfusion cells/scaffold factory for scale-up production.
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