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Roberta Gomes Coelho,Neli Kika Honda,Maria do Carmo Vieira,Rosenei Louzada Brum,Fernando Roge´rio Pavan,Clarice Queico Fujimura Leite,Claudia Andre´a Lima Cardoso 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
Bromelia balansae (Family Bromeliaceae) is a medicinal plant commonly used in the central region of Brazil as a cough syrup and also eaten roasted. The methanolic extract of ripe fruits was analyzed by chromatographic methods and spectrometrically. Four glycoside flavonols were isolated: kaempferol-3-O-α-l-rhamnopyranoside (1), kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (2), quercetin-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (3), and kaempferol 3,7-di-O-α-l-rhamnopyranoside (4). The resazurin microtiter assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis. The results showed a moderate activity of the methanolic extract with a minimal inhibitory concentration of 128μg/mL. Antioxidant activity was evaluated as free radical scavenging capacity and inhibition of peroxidation. Free radical scavenging capacity was assessed by measuring the scavenging activity of methanolic extract and methanolic fraction on 2,2-diphenyl-1-picrylhydrazyl radical. The methanolic extract showed low values of antioxidant activities, whereas the methanolic fraction exhibited free radical scavenging activity ranging from 20.2% to 91.1%, and the inhibition of peroxidation values ranging from 5.6% to 27.5%. This is the first chemical study reported in the literature about this species.
Claudia Andréa Lima Cardoso,Gustavo Ruivo Salmazzo,Neli Kika Honda,Crislaine Batista Prates,Maria do Carmo Vieira,Roberta Gomes Coelho 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
The antimicrobial activity of the hexanic extracts and fractions of fruits of Campomanesia pubescens pubescens (D.C) O. Berg and Campomanesia adamantium (Cambess.) O. Berg were assayed against six microorganisms. The hexanic extracts of C. pubescens and C. adamantium fruits were also investigated in their composition by gas chromatography-mass spectrometry and high-performance liquid chromatography with diode array detection. The results of this study showed the presence of 34 volatile compounds and flavonoids in the hexanic extract of C. pubescens and 33 volatile compounds and flavonoids in the hexanic extract of C. adamantium.
Ana Paula Corrêa Castelo Branco Nappi,Roberta Gomes Coelho,Neli Kika Honda,Catharine Ferrazoli,Arnildo Pott,Clélia Akiko Hiruma-Lima 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6
The present study reveals the pharmacological action of Serjania erecta Radlk. (Family Sapindaceae), an important medicinal plant species used in the Brazilian Pantanal against gastric pain. The methanolic (Me) and chloroformic (Se) extracts obtained from leaves of S. erecta were challenged by a very strong necrotizing agent in rodents, absolute ethanol. Se was also confronted with a nitric oxide synthase inhibitor (NG-nitro-l-arginine methyl ester), a capsaicin cation channel transient receptor potential vanilloid type 1 antagonist (ruthenium red), or a sulfhydryl-blocker (N-ethylmaleimide) to evaluate the participation of these cytoprotective factors in gastroprotection. In an in vivo experimental model, Me and Se presented several degrees of gastroprotective action without signs of acute toxicity. The best gastroprotective effect was restricted to all doses of Se. The mechanisms involving the gastroprotective action of Se are related to an augmented defense mechanism of the gastrointestinal mucosa consisting of sensory neurons, nitric oxide, and sulfhydryl groups that prevent and attenuate the ulcer process. The presence of polyisoprenoids in the Se explains the potent gastroprotective action of this medicinal species. Effective gastroprotective action and the absence of acute toxicity indicate this species may be a promising herbal drug against gastric disease.