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        大麥의 半矮性 短稈 및 GA反應性의 遺傳 Ⅰ. 半矮性 短桿形質의 遺傳分析

        Nam Chon Paek(白南天),Moo Hee Yang(楊武熙),Jai Wook Shim(沈載昱) 한국육종학회 1987 한국육종학회지 Vol.19 No.3

        This investigation was carried out to identify dwarfing sources of five Korean barley cultivars, Baekdong, Namhae, Songhak, Paldal, and Dusan 8. Three foreign cultivars, Himalaya, Svanhals, and Baitori 10 were used as the genetic markers of the dwarf genes, br, br₂ and uz respectively. Eight cultivars were crossed by all possible combinations so as to know the genetic sources of their culm lengths. The major dwarf gene of Baekdong was the uz by which Baitori 10 was controlled. Songhak was controlled by two independent recessive dwarf genes and one of them was considered to be uz gene. The crosses between Korean cultivars, Namhae, Paldal and Dusan 8, and the cultivars of three genetic markers showed that three Korean cultivars were controlled by one recessive dwarf gene different from br,br₂ and uz gene. In the crosses among three Korean cultivars, Namhae, Paldal, Dusan 8, each one was controlled by different gene or genes from another ones.

      • 광발아성을 갖는 잡초성 벼의 발견

        남천 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-

        Photoregulation of seed germination is the important character of plant species producing small seeds such as lettuce, tobacco, Arabidopsis, and Gramineae weeds, etc. It has been known that there is no photoblastism in rice seeds. Here we present at first that a photoblastic rice (PBR) seed was discovered among Korean weedy rice lines. The PBR seed identified among the 235 weedy rice lines germinated about 99% under white light and 0.3% in total darkness. When the PBR seeds were planted, it showed the variation of emergency rate according to the burial depth and temperature. In the 18/12℃ (day/night) diurnal condition, the emergency rate was 39% at 1 cm burial depth, 15% at 3~5cm, 5% at 7cm, and 1% at 10 cm. The deeper the seeds were planted, the lower the emergency showed. Under 24/22℃ condition, the emergency rate at 1 cm burial depth was only 6%. When the PBR seeds were stored at room temperature after harvest, seed dormancy and photoblastism were gradually decayed. The seed dormancy disappeared more than 90% after 3-month storage. Photoblastism disappeared 26% after 16 months and 63% after 28 months. The seed dormancy of the PBR was broken by high temperature(50℃ for 7days) to dry seeds and, however, the photoblastism was not influenced and still maintained. In the amount of light and imbibition time, the more the seeds were irradiated, the higher germination rate was. The more imbibition time before light was, the more germination was delayed. In the response of light quality, the germination rate of the PBR seeds was promoted by red light and inhibited by far-red light, indicating that the seed germination was controlled by phytochromes. It also showed photoreversibility. The seed germination of PBR was alternately promoted or inhibited by the final irradiation of red or far-red light.

      • Barley yellow dwarf virus의 분자학적 동정 및 밀에서의 저항성 유전자원 탐색

        남천 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-

        Barley Yellow Dwarf Virus (BYDV), an aphid-borne luteovirus, is a major plant pathogenic disease causing a huge economic loss in the grain production of a wide range of Gramineae species throughout the world. It has been recently reported that BYDV also occurred frequently in wheat field in Korea. We performed to develop the detection and classification methods of BYDV strains that were accomplished by reverse transcription-polymerase chain reaction (RT-PCR), to investigate the regional distributions of BYDV strains in Korea, and to identify the resistant cultivars or lines of wheat to the predominant BYDV strains, providing basic information for the breeding of BYDV-resistant wheat varieties. Since there are high variations among BYDV strains, three pairs of primers were designed to detect BYDV strains such as PAV(Vic-PAV and CN-PAV) and MAV (primer A) simultaneously, specifically Vic-PAV (primer B), and MAV (primer C) based on the genomic RNA sequences of BYDV strains reported previously. The validity of the primers was confirmed using several BYDV strains obtained from CIMMYT. Though three BYDV strains were able to be detected using primer A, PCR products were not distinguished between two PAV strains. It was possible to separate them with a restriction enzyme, EcoRI, whose restriction site was present in the amplified DNA fragment from Vic-PAV, but not from CN-PAV. Using these methods, the regional distribution of BYDV strains in Korea from 1999 to 2000 showed that PAV strain was mainly detected about 65% (Vic-PAV 52.6%; CN-PAV 47.4%) and MAV strain about 3%. Using ELISA test for the examination of BYDV resistance with 17 cultivars and 4 lines among Korean wheat, three cultivars, Gurumil, Topdongmil, and Olgurumil, were susceptible to BYDV and the others were resistant. In plant growth and yield component responses to BYDV infection, Gurumil showed significant difference between the uninfected and the infected, suggesting the most susceptible to BYDV among Korean wheat, but Eunpamil and Seohae118 did no difference, an indication that they have the highest resistance.

      • Isolation, characterization, and genetic mapping of stay-green mutant in rice

        Paek, Nam-Chon 서울대학교 농업개발연구소 2001 농업생명과학연구 Vol.5 No.-

        Leaf color turns yellow during senescence due to the degradation of chlorophylls and photosynthetic proteins. A stay-green mutant was isolated from the glutinous japonica rice Hwacheong-wx through N-methyl-N-nitrosourea mutagenesis. Leaves of the mutant remained green while turning yellow in those of the wild-type rice during senescence. The stay-green phenotype was controlled by a single recessive nuclear gene, tentatively symbolized as sgr(t). All the phenotypic characteristics of the mutant were the same as those of the wild-type lines except for the stay-green trait. Leaf chlorophyll concentration of the mutant was similar to that of the wild type before heading, but decreased steeply in the wild type during grain filling, while very slowly in the mutant. However, no difference in photosynthetic activity was observed between the stay-green mutant and the yellowing wild-type leaves, indicating that senescence is proceeding normally in the mutant leaves and the mutation affects the rate of chlorophyll degradation during the leaf senescence. Using phenotypic and molecular markers, we mapped the sgr(t) locus to the long arm of chromosome 9 between RFLP markers RG662 and C985 at 1.8 and 2.1 cM intervals, respectively.

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