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Melatonin improves antioxidant defense mechanism of basil under drought stress
Naghizadeh Mehdi,Reiter Russel J.,Kabiri Rozita,Moradi Rooholla 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1
Basil ( Ocimum basilicum L.) is a highly important medicinal plant. In this study, the interactional eff ects of water defi cit and melatonin application on growth and physio-biochemical characteristics of basil were investigated. The treatments included three levels of irrigation (100, 75 and 50% of fi eld capacity) and three melatonin concentrations (0, 50 and 100 μmol). Drought stress decreased the dry weight of plants but application of 100 μmol melatonin markedly increased dry weight under these conditions. Melatonin profoundly enhanced plant drought tolerance by stimulating the antioxidant enzymes (catalase, phenylalanine ammonia-lyase, ascorbate peroxidase and polyphenol oxidase) activities and secondary metabolites (total phenol, fl avonoid and anthocyanin contents). Furthermore, melatonin application reduced lipid peroxi dation and increased the relative water content and photosynthetic pigments, resulting in an improvement of plant dry matter. It is concluded that foliar spraying with melatonin induced the pre-adaptive responses to drought stress followed by regulating antioxidant defense mechanisms.
Larijani, Mona Sadat,Bahiraei, Narges,Nikbin, Mehri,Mohajel, Nasir,Rad, Leila Naghizadeh,Baghbani, Fahimeh,Mapar, Maryam,Sadat, Seyed Mehdi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8
Infection with the hepatitis C virus is a major public health concern which can lead to carcinoma and liver failure. It has been shown that single nucleotide polymorphisms can affect the level of gene activity of tumor necrosis factor (TNF) which has an important role, especially in viral infections which can lead to apaptosis of infected hepatocellular cells. We investigated the impact of three possible genotypes for rs1800629 or A/G single nucleotide polymorphism located downstream of $TNF{\alpha}$ gene promoter in groups of control (n=76) and chronic hepatitis C patients (n=89) focusing on the response to treatment among sensitive and resistant groups. Genomic DNA was extracted from $500{\mu}l$ prepheral whole blood and PCR and RFLP were used to amplify the region of interest and genotyping. With statistical analyzes a p-value <0.05 was considered meaningful. There was no significant difference in distribution of possible three genotypes among healthy individuals and patients (P=0.906, OR=1.194, CI=0.063-22.790). However, the frequency of G allele was higher in patients whereas A allele was more common among healthy individuals (p<0.0001). Further studies with more samples seem to be necessary.