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- 저자 : Nagarajan Kayalvizhi (검색결과 2 건)
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Nagarajan Kayalvizhi,Paramasamy Gunasekaran 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2
A bacterial strain Bacillus licheniformis MKU3,isolated from slaughterhouse sediments showed a strong antimicrobial activity. The antimicrobial substance produced by this strain was found to be a protein that inhibited a broad range of bacterial strains, such as Bacillus sp.,Staphylococcus sp., Streptococcus sp., and Listeria monocytogenes. The antimicrobial peptide was purified to homogeneity by cut off membrane filtration followed by gel filtration chromatography. The purified protein with low molecular mass (< 8 kDa) was resolved as single band on Tricine SDS-PAGE. This protein was stable at 100oC for 10 min, but lost its activity at 121oC in 15 min. It was resistant to the proteolytic action of trypsin, proteinase K,and pronase E and stable within a wide range of pH (3.0~11.0). This protein exhibited lytic activity on selected indicator strain Kurthia gibsonii GCS6.
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( Rameshkumar ),( Neelamegam ),( Niraikulam Ayyadurai ),( Nagarajan Kayalvizhi ),( Paramasamy Gunasekaran ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
The genetic diversity of plant growth-promoting rhizobacterial (PGPR) fluorescent pseudomonads associated with the sugarcane (Saccharum officinarum L.) rhizosphere was analyzed. Selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. Furthermore, 16S rDNA sequence analysis was performed to identify and differentiate these isolates. Based on 16S rDNA sequence similarity, the isolates were designated as Pseudomonas plecoglossicida, P. fluorescens, P. libaniensis, and P. aeruginosa. Differentiation of isolates belonging to the same group was achieved through different genomic DNA fingerprinting techniques, including randomly amplified polymorphic DNA (RAPD), amplified ribosomal DNA restriction analysis (ARDRA), repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC), and bacterial repetitive BOX elements (BOX) analyses. The genetic diversity observed among the isolates and rep-PCR-generated fingerprinting patterns revealed that PGPR fluorescent pseudomonads are associated with the rhizosphere of sugarcane and that P. plecoglossicida is a dominant species. The knowledge obtained herein regarding the genetic and functional diversity of fluorescent pseudomonads associated with the sugarcane rhizosphere is useful for understanding their ecological role and potential utilization in sustainable agriculture.
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