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Increased extracellular matrix density disrupts E-cadherin/β-catenin complex in gastric cancer cells
Jang, Minjeong,Koh, Ilkyoo,Lee, Jae Eun,Lim, Ju Yeon,Cheong, Jae-Ho,Kim, Pilnam The Royal Society of Chemistry 2018 Biomaterials Science Vol.6 No.10
<P>During gastric cancer (GC) progression, increased extracellular matrix (ECM) deposition, notably collagen type I, correlates with an overall increase in expression of the mesenchymal phenotype. In GC tissue, the intestinal epithelium exhibits impaired cell-cell adhesion and enhanced cell-ECM adhesion. The alteration of intercellular integrity is one of tumorigenesis feature including tumor invasion and metastasis. Using a density-varying ECM, we studied the effect of ECM density on both intercellular- and ECM-interactions according to alterations of ECM-mediated signaling. A dense collagen matrix increases integrin-mediated cell-ECM interactions with phosphorylated FAK and ERK signaling in human gastric adenocarcinoma cells (AGS, MKN74), which regulates GC proliferation and the chemotherapeutic response. In addition, GC cells exhibited a disrupted membranous E-cadherin/β-catenin complex and, remarkably, showed cytoplasmic or nucleic localization of β-catenin in response to collagen density. Furthermore, we found that membranous E-cadherin/β-catenin complex could be recovered by inhibiting the phosphorylation of FAK, which in turn influences the chemotherapeutic effect. These results provide insight into how matrix density differentially regulates cancer cell phenotype and may have significant implications for the design of biomaterials with appropriate physical properties for <I>in vitro</I> tumor models.</P>
Membrane Progesterone Receptors in the Porcine Uterine Endometrium
Minjeong Kim,Inkyu Yoo,Jisoo Han,Hwanhee Jang,Hakhyun Ka 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
Progesterone regulates endometrial functions to support implantation, placentation, and fetal/placental development in the uterus. It is known that actions of progesterone are mediated by nuclear progesterone receptor (PGR), using the signaling pathway referred to the genomic pathway. However, all physiological progesterone actions cannot be explained by the genomic pathway via PGR, and it is understood that there are non-genomic actions of progesterone though membrane progesterone receptors, progesterone receptor membrane components (PGRMCs) and progestin and adipoQ receptors (PAQRs). The expression and localization of PGRMCs and PAQRs has been reported in female reproductive tissues of several species such as human, mouse and cattle. Previously, we have shown that PGRMCs and PAQRs are expressed in the porcine uterine endometrium during the estrous cycle and pregnancy. However, the regulatory mechanism for expression of PGRMCs and PAQRs in the uterine endometrium has not been studied in pigs. Thus, to understand the regulatory mechanism of PGRMC1, PGRMC2, PAQR5, PAQR6, PAQR7, PAQR8, and PAQR9 expression in the uterine endometrium during the estrous cycle and pregnancy in pigs, we determined the effect of steroid hormones estrogen and progesterone on expression of PGRMCs and PAQRs using the endometrial tissue explants for immature pigs. Levels of PGRMC1, PGRMC2, PAQR5, PAQR6, PAQR7, PAQR8, and PAQR9 mRNAs were increased by increasing doses of progesterone, but not by estradiol in the uterine endometrium. Blocking PGR by treatment of RU486, a progesterone receptor antagonist, increased levels of endometrial PGRMCs and PAQRs mRNA. These data showed that membrane progesterone receptors were induced by progesterone in the uterine endometrium, suggesting that these membrane progesterone receptors may play an important role in mediating progesterone actions in the uterine endometrium for regulation of the estrous cyclicity and pregnancy. [Supported by the Next Generation Biogreen 21 Program (# PJ01119103), Rural Development Administration, Republic of Korea]
Efflux Attenuates the Antibacterial Activity of Q203 in <i>Mycobacterium tuberculosis</i>
Jang, Jichan,Kim, Ryangyeo,Woo, Minjeong,Jeong, Jinsun,Park, Da Eun,Kim, Guehye,Delorme, Vincent American Society for Microbiology 2017 Antimicrobial Agents and Chemotherapy Vol.61 No.7
<P>New and improved treatments for tuberculosis (TB) are urgently needed. Recently, it has been demonstrated that verapamil, an efflux inhibitor, can reduce bacterial drug tolerance caused by efflux pump activity when administered in combination with available antituberculosis agents. The aim of this study was to evaluate the effectiveness of verapamil in combination with the antituberculosis drug candidate Q203, which has recently been developed and is currently under clinical trials as a potential antituberculosis agent. We evaluated changes in Q203 activity in the presence and absence of verapamil in vitro using the resazurin microplate assay and ex vivo using a microscopy-based phenotypic assay for the quantification of intracellular replicating mycobacteria. Verapamil increased the potency of Q203 against Mycobacterium tuberculosis both in vitro and ex vivo, indicating that efflux pumps are associated with the activity of Q203. Other efflux pump inhibitors also displayed an increase in Q203 potency, strengthening this hypothesis. Therefore, the combination of verapamil and Q203 may be a promising combinatorial strategy for anti-TB treatment to accelerate the elimination of M. tuberculosis.</P>
A Case Report of C-ANCA Positive Bartonella Endocarditis Associated Glomerulonephritis
( Minjeong Kim ),( Ha Nee Jang ),( Dae-hong Jeon ),( Hyun Seop Cho ),( Se-ho Chang ),( Dong Jun Park ),( Jeong Rang Park ),( Dae Hyun Song ),( Oh-hyun Cho ),( Hyun-jung Kim ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Background: Bartonella endocarditis is very rare in South Korea. Some bacterial endocarditis can involve the kidney. This is the first report of C-ANCA positive Bartonella endocarditis-associated glomerulonephritis in South Korea. Case presentation: A 67-year-old man complained 7kg weight loss with anorexia over the past 6 months. Serum creatinine was 4.35 mg/dL. Albumin/globulin ratio was reversed and the ratio of spot urine protein/creatinine was 1.1. Bone marrow exam showed normocellular marrow without monoclonality of plasma cells. There were two old vegetations on transesophageal echocardiography. Blood cultures of two couple were negative. The renal histology findings demonstrated global sclerosis, mesangial proliferation, crescent formation and diffuse glomerular capillary walls thickened with deposits of C3, IgM and C1q. The level of C3 was decreased to 43.9 mg/dL and C-ANCA was positive. In further history findings, he has drunken deer blood. So, We examed Q fever, Legionella and Bartonella antibody test and Bartonella IgG ab was elevated to 1: 2,048. Azotemia and proteinuria were not improved though doxycycline and rifampin treatment for 8 weeks. But these were improved after steroid therapy for 6 weeks with same antibiotics. Conclusions: It is necessary to evaluate rare organism like Bartonella infection in the BCNE. In addition, if there are azotemia and proteinuria, we should consider renal biopsy to rule out the renal complication of endocarditis-associated glomerulonephritis and for early aggressive treatment.
Jang, Bora,Kim, Boyoung,Kim, Hyunsook,Kwon, Hyokyoung,Kim, Minjeong,Seo, Yunmi,Colas, Marion,Jeong, Hansaem,Jeong, Eun Hye,Lee, Kyuri,Lee, Hyukjin American Chemical Society 2018 NANO LETTERS Vol.18 No.7
<P>Enzymatic synthesis of RNA nanostructures is achieved by isothermal rolling circle transcription (RCT). Each arm of RNA nanostructures provides a functional role of Dicer substrate RNA inducing sequence specific RNA interference (RNAi). Three different RNAi sequences (GFP, RFP, and BFP) are incorporated within the three-arm junction RNA nanostructures (Y-RNA). The template and helper DNA strands are designed for the large-scale in vitro synthesis of RNA strands to prepare self-assembled Y-RNA. Interestingly, Dicer processing of Y-RNA is highly influenced by its physical structure and different gene silencing activity is achieved depending on its arm length and overhang. In addition, enzymatic synthesis allows the preparation of various Y-RNA structures using a single DNA template offering on demand regulation of multiple target genes.</P> [FIG OMISSION]</BR>