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Effect of Accelerated Aging on the Color Stability of Dual-Cured Self-Adhesive Resin Cements
Kim, Ah-Rang,Jeon, Yong-Chan,Jeong, Chang-Mo,Yun, Mi-Jung,Huh, Jung-Bo Korean Academy of Dental Science 2015 Journal of korean dental science Vol.8 No.2
Purpose: The effect of accelerated aging on color stability of various dual-cured self-adhesive resin cements were evaluated in this study. Materials and Methods: Color stability was examined using three different brands of dual-cured self-adhesive resin cements: G-CEM LinkAce (GC America), MaxCem Elite (Kerr), and PermaCem 2.0 (DMG) with the equivalent color shade. Each resin cement was filled with Teflon mold which has 6 mm diameter and 2 mm thickness. Each specimen was light cured for 20 seconds using light emitting diode (LED) light curing unit. In order to evaluate the effect of accelerated aging on color stability, color parameters (Commission Internationale de l'Eclairage, CIE $L^*$, $a^*$, $b^*$) and color differences (${\Delta}E^*$) were measured at three times: immediately, after 24 hours, and after thermocycling. The $L^*$, $a^*$, $b^*$ values were analyzed using Friedman test and ${\Delta}E^*$ values on the effect of 24 hours and accelerated aging were analyzed using t-test. These values were compared with the limit value of color difference (${\Delta}E^*=3.7$) for dental restoration. One-way ANOVA and Scheff's test (P<0.05) were performed to analyze each ${\Delta}E^*$ values between cements at each test period. Result: There was statistically significant difference in comparison of color specification ($L^*$, $a^*$, $b^*$) values after accelerated aging except $L^*$ value of G-CEM LinkAce (P<0.05). After 24 hours, color difference (${\Delta}E^*$) values were ranged from 2.47 to 3.48 and $L^*$ values decreased and $b^*$ values increased in all types of cement and MaxCem Elite had high color stability (P<0.05). After thermocycling, color change's tendency of cement was varied and color difference (${\Delta}E^*$) values were ranged from 0.82 to 2.87 and G-CEM LinkAce had high color stability (P<0.05). Conclusion: Color stability of dual-cured self-adhesive resin cements after accelerated aging was evaluated and statistically significant color changes occurred within clinically acceptable range.
Kim, Heui-Soo,Kim, Dae-Soo,Huh, Jae-Won,Ahn, Kung,Yi, Joo-Mi,Lee, Ja-Rang,Hirai, Hirohisa Korean Society for Molecular Biology 2008 Molecules and cells Vol.26 No.1
We characterized the human endogenous retrovirus (HERV-W) family in humans and primates. In silico expression data indicated that 22 complete HERV-W families from human chromosomes 1-3, 5-8, 10-12, 15, 19, and X are randomly expressed in various tissues. Quantitative real-time RT-PCR analysis of the HERV-W env gene derived from human chromosome 7q21.2 indicated predominant expression in the human placenta. Several copies of repeat sequences (SINE, LINE, LTR, simple repeat) were detected within the complete or processed pseudo HERV-W of the human, chimpanzee, and rhesus monkey. Compared to other regions (5'LTR, Gag, Gag-Pol, Env, 3'LTR), the repeat family has been mainly integrated into the region spanning the 5'LTRs of Gag (1398 bp) and Pol (3242 bp). FISH detected the HERV-W probe (fosWE1) derived from a gorilla fosmid library in the metaphase chromosomes of all primates (five hominoids, three Old World monkeys, two New World monkeys, and one prosimian), but not in Tupaia. This finding was supported by molecular clock and phylogeny data using the divergence values of the complete HERV-W LTR elements. The data suggested that the HERV-W family was integrated into the primate genome approximately 63 million years (Myr) ago, and evolved independently during the course of primate radiation.
Kim, Ah-Rang,Jeon, Yong-Chan,Jeong, Chang-Mo,Yun, Mi-Jung,Choi, Jae Won,Kwon, Yong Hoon,Huh, Jung-Bo JAPANESE SOCIETY FOR DENTAL MATERIALS AND DEVICES 2016 Dental materials journal Vol.35 No.2
<P>The purpose of this study was to compare the compressive strength, diametral tensile strength and microhardnss of several self-adhesive resin cements (Rely-X U200, Clearfill SA Luting, G-CEM LinkAce, Maxcem Elite, PermaCem 2.0, and Zirconite) using different activation modes (self-cured, light -cured) and testing time (immediately, 24 h, thermocycling). Specimens were prepared for the compressive strength (empty set 4x6 mm) and diametral tensile strength and microhardness (empty set 6x3 mm) according to ISO standards. The strength after 24 h was higher than immediately after. In addition, G-CEM showed the highest values. In terms of the activation modes, Rely-X U200, PermaCem 2.0 had higher values in the light-curing than the self-curing. In conclusion, all cements demonstrated clinically available strength values and revealed differences in strength according to their composition, testing time and activation mode. Furthermore, correlation was found between the microhardness (degree of conversion) and mechanical strengths of the cements tested.</P>
Kim, So-Yoen,Kim, Jin-Hyoung,Son, Mi Rang,Yi, Seungjun,Kim, Chul Hoon,Son, Ho-Jin,Kang, Sang Ook American Chemical Society 2019 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.123 No.31
<P>In this study, we prepared phenylimidazole-based C^N-cyclometalated Ir(III) complexes (<B>DMP</B>, <B>TPF2</B>) and a C^C-cyclometalated Ir(III) complex (<B>PMP</B>), and investigated the energy transfer process by examining the intermolecular interactions between the two cyclometalated Ir(III) complexes. In films doped with 3% Ir(C^C)<SUB>3</SUB> complex (<B>PMP</B>) and 15% Ir(C^N)<SUB>3</SUB> complex (<B>DMP</B> or <B>TPF2</B>), the <B>PMP</B> effectively induced energy transfer to the <B>DMP</B> or <B>TPF2</B>. This intermolecular energy transfer process was investigated using a picosecond time-resolved emission spectroscopic method. In the case of mixing <B>PMP</B> with <B>DMP</B>, where two types of luminescence were observed at 470 and 580 nm, the emission at 470 nm was due to <B>DMP</B>, while the emission at 580 nm can be assigned as the intermolecular exciplex emission. By contrast, in the case of mixing <B>PMP</B> with <B>TPF2</B>, the emission at 465 nm corresponding to the <B>PMP</B> emission region decreased for 18.5 ns, while the emission at 530 nm corresponding to <B>TPF2</B> increased. This emission can be attributed to the energy transfer from <B>PMP</B> to <B>TPF2</B>. In addition, no change was observed in the longer wavelength region than the <B>TPF2</B> emission region for 10 μs. We analyzed the energy transfer process when <B>PMP</B> was added to the dopant (<B>DMP</B> and <B>TPF2</B>) and found that <B>TPF2</B> was more efficient than <B>DMP</B> in the device without <B>PMP</B> doping, but it showed performance deterioration in high current density (>1 mA/cm<SUP>2</SUP>) owing to activation of fluorinated ligands. Finally, it was confirmed that the operation lifetime and efficiency of the device were improved by doping 3% of <B>PMP</B> in emissive layer (EML).</P> [FIG OMISSION]</BR>
Kim, Ok Mi,Kim, Hyun Jeong,Kim, Sang Dal,Park, Dong Chul,Lee, Kap Rang 한국미생물 · 생명공학회 1995 Journal of microbiology and biotechnology Vol.5 No.5
The ddh gene encoding meso-diaminopimelate (meso-DAP)-dehydrogenase (DDH) in Brevibacterium lactofermentum was isolated by complementation of the Escherichia coli dapD mutation. It was supposed from subcloning experiments and complementation tests that the evidence for DDH activity appeared in about 2.5 kb Xhol fragmented genome. The 2.5 kb Xhol fragment containing the ddh gene was sequenced, and an open reading frame of 960 by encoding a polypeptide comprising 320 amino acids was found. Computer analysis indicated that the deduced amino acid of the B. lactofermentum ddh gene showed a high homology with that of the Corynebacterium glutamicum ddh gene.
Kim, Su-Jin,Kim, Ye-Jin,Lee, Jae-Ho,Oh, Sa-Rang,Park, Chan-Ik,Jeong, Ji-Wook,Um, Jae-Young,Hong, Seung-Heon,Ahn, Eun-Mi 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3
Genistein-4'-O-${\alpha}$-L-rhamnopyranosyl-(1-2)-${\beta}$-D-glucopyranoside (GRG) is one of constituents isolated from $Sophora$ $japonica$ (Leguminosae). It is known to exert various effects such as anti-inflammatory effect. However, the anti-allergic effects of GRG and its molecular mechanisms have yet to be clearly elucidated. In this study, we attempted to evaluate the effects of GRG on mast cell-mediated allergic inflammation in vitro and in vivo. We investigated to ascertain the pharmacological effects of GRG on compound 48/80-induced systemic anaphylaxis and experimental scratching behavior in mice. Additionally, to find a possible explanation for the anti-allergic mechanisms of GRG, we evaluated the regulatory effects of GRG on the level of inflammatory mediators in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that GRG reduced compound 48/80-induced systemic anaphylaxis and scratching behavior in mice. Additionally, GRG inhibited the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-8 and histamine as well as the activation of caspase-1 in PMACI-stimulated HMC-1. Taken together, the findings of this study provide a novel insight into the pharmacological actions of GRG as a potential molecule for use in the treatment of allergic inflammation diseases.
Kim, Young Hwa,Im, A-Rang,Park, Bo-Kyung,Paek, Seung Ho,Choi, Goya,Kim, Yu Ri,Whang, Wan Kyunn,Lee, Kang Hee,Oh, Seung-Eun,Lee, Mi Young Hindawi 2018 BioMed research international Vol.2018 No.-
<P><I>Hibiscus syriacus </I>L. (Malvaceae) is an important ornamental shrub in horticulture and has been widely used as a medical material in Asia. The aim of this study was to assess the antidepressant and neuroprotective effects of a root bark extract of<I> H. syriacus </I>(HSR) and to investigate the underlying molecular mechanisms. Using an animal model of restraint stress, we investigated the effects of HSR on depressive-like behaviors and on the expression levels of serotonin, corticosterone, and neurotrophic factors in the brain. The mice were exposed to restraint stress for 2 h per day over a period of 3 weeks and orally treated with HSR (100, 200, or 400 mg/kg/day). We also examined the neuroprotective effect of HSR using corticosterone-treated human neuroblastoma SK-N-SH cells. The cells were incubated with the extract for 24 h, followed by corticosterone stimulation for 1 h, and then cell viability assay, cellular ATP assay, mitochondrial membrane potential (MMP) assay, cellular reactive oxygen species (ROS) assay, and western blotting were used to investigate the neuroprotective effects of HSR. Administration of HSR not only reduced the immobility times of the restraint-stressed mice in the forced swimming and tail suspension tests, but also significantly increased sucrose preference in the sucrose preference test. In addition, HSR significantly reduced the plasma levels of corticosterone and increased the brain levels of serotonin. The extract also increased the phosphorylation level of cyclic AMP response element-binding (CREB) protein and the expression level of brain-derived neurotrophic factor (BDNF). The in vitro assays showed that HSR pretreatment increased cell viability and ATP levels, recovered MMP, decreased ROS levels, and increased the expression of CREB and BDNF in corticosterone-induced neurotoxicity. Taken together, our data suggest that HSR may have the potential to control neuronal cell damage and depressive behaviors caused by chronic stress.</P>