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- 저자 : Liying Niu (검색결과 2 건)
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Liying Niu,Dajing Li,Chunquan Liu,Fuguo Liu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3
Lipoxygenases (LOX) in milk-stage sweet corn and waxy corn were extracted using a phosphate buffer (pH 7.0) and enzyme activities were determined using linoleic acid as a substrate. Michaelis constant (Km) values, decimal reduction times (D value), temperature sensitivity parameters (Z value), and activation energies (Ea) were calculated. Enzymes from both corn types followed first-order inactivation kinetics within 0-25 min and 50- 70℃. However, enzymes exhibited different pH profiles and affinities toward linoleic acid. Km values (4.34 and 1.40 mM for sweet corn and waxy corn, respectively), heat stability values, and Ea values (116.81 and 246.82 kJ/mol) were different. Waxy corn LOX was more heat stable below 65℃ with a higher D value, but was more temperature sensitive with a lower Z value. The different characteristics suggested the presence of different isoenzymes and necessitated the use of different parameters for blanching.
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Differential Response of Two Contrasting Melon (Cucumis melo L.) Genotypes to Drought Stress
Asad Rehman,Jinyang Weng,Pengli Li,Jing Yu,Saeed ur Rahman,Muhammad Khalid,Iftikhar Hussain Shah,Shazma Gulzar,Liying Chang,Qingliang Niu 한국식물학회 2023 Journal of Plant Biology Vol.66 No.6
The present study aims to gain insights into the response mechanisms of drought stress tolerance among two contrasting melon (Cucumis melo L.) genotypes. Drought stress was imposed by polyethylene glycol (10%) for 7 days and various physiochemical and molecular characteristics were analyzed on different days of drought stress treatment. Results revealed that the drought-sensitive genotype (MG-II) was significantly affected by drought stress, as evidenced from the elevation in hydrogen peroxide (H2O2), malondialdehyde content (MDA), and electrolyte leakage (EC). Furthermore, drought stress significantly hindered the vegetative growth, chlorophyll fluorescence, photosynthetic pigments, and leaf gas exchange characteristics of MG-II genotypes. In contrast, the drought-resistant genotype (MG-I) exhibited a robust response to drought stress, characterized by marked upregulation in the antioxidant enzyme genes and activities, which in turn resulted in a decrease in oxidative damage and improved vegetative growth and photosynthetic functions. Additionally, transmission electron microscopy (TEM) revealed that the oxidative damage triggered by drought stress was more severe in the MG-II genotype, which exhibited an irregular chloroplast shape compared to the MG-I. These findings may potentially enhance our comprehension of coping strategies involved in drought stress tolerance and provide materials for future melon breeding and molecular studies.
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