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        A Novel Cold-adapted Endoglucanase (M6A) from Microbacterium kitamiense S12 Isolated from Qinghai-Tibetan Plateau

        Ling Lin,Na Qin,Linyan Guan 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.3

        The gene M6A coding a novel cold-adapt endoglucanase was cloned from Microbacterium kitamiense Sa12 isolated from a wasteland in Saga, Qinghai-Tibetan Plateau. The deduced protein sequence encoded a 411residue polypeptide sharing similar identities with glycosyl hydrolase family 6 enzymes. The recombinant M6A displayed maximum hydrolysis activity of 1.51 U/mg toward soluble cellulose substrate, CMC at 35°C and pH 5.0, and the Km and Vmax value were 2.12 mg/mL and 15.33 μmol/min*mg, respectively. Interestingly, M6A exhibited significant activity even at ice cold condition, showing 30-40% relative activity at 0-5°C, and had good tolerance to Li+, K+, NH4+, Rb+, Ca2+, Mg2+, Zn2+, Mn2+, and Ni2+, expect divalent cation Cu2+ led to 30% residual activity. These properties might make M6A to be a promising candidate used in the psychrophilic industrial process and/ or the volatile and thermosensitive manufacturing process.

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