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RISS 인기검색어
- 검색키워드
- 저자 : Linglong Liu (검색결과 4 건)
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Genetic dissection of leaf-related traits using 156 chromosomal segment substitution lines
Xi Liu,Linglong Liu,Yinhui Xiao,Shijia Liu,Yunlu Tian,Liangming Chen,Zhiquan Wang,Ling Jiang,Zhigang Zhao,Jianmin Wan 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6
A two-line super-hybrid rice (Oryza sativa L.) variety [Liangyoupei9 (LYP9)] demonstrated superiority over its both parents, viz. elite inbred lines 93-11 and Pei-ai64S (PA64S), as well as other conventional hybrids, and had long been exploited in China. However, the genetic basis of its leaf-related traits, supposed to be an important component for yield potential, remains elusive. Here, initially a set of chromosome segment substitution lines (CSSLs) was constructed, in which the genome of Pei-ai64S has been introgressed into the background of 93-11. This set was developed by marker aided selection, based on 123 polymorphic SSR markers. The introgressed chromosomal segments presented in the 156 CSSLs covered 96.46% of Pei-ai64S genome. Afterwards, the CSSLs were deployed to assess the genetic basis of leaf size (length and width) and chlorophyll content of top three leaves across five different environments. The CSSLs showed transgressive segregation for all of the traits, and significant correlations were detected among most of the traits. A total of 27 quantitative trait loci (QTL) were identified on ten chromosomes, and three QTL cluster affecting related traits were found on chromosome 3, 6, and 8, respectively. Remarkably, two key QTLs, qALW3-1 and qALW3-2, both controlling the antepenultimate leaf width, were identified in all five environments, and their effect were further validated by CSSLs harboring the two QTL alleles. Our results indicate that developing CSSLs is a powerful tool for genetic dissection of quantitative traits. Meanwhile, the QTLs controlling leaf-related traits uncovered here provide useful information for marker-assisted selection in improving the performance of leaf morphology and photosynthetic ability.
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Wuhua Long,Bangning Dong,Yihua Wang,Pengyi Pan,Yunlong Wang,Linglong Liu,Xiaoli Chen,Xi Liu,Shijia Liu,Yunlu Tian,Liangming Chen,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Cereal opaque-kernel mutants are ideal geneticmaterials for studying the mechanism of starch biosynthesisand amyloplast development. Here we isolated and identifiedtwo allelic floury endosperm 8 (flo8) mutants of rice, namedflo8-1 and flo8-2. In the flo8 mutant, the starch content wasdecreased and the normal physicochemical features ofstarch were altered. Map-based cloning and subsequentDNA sequencing analysis revealed a single nucleotidesubstitution and an 8-bp insertion occurred in UDP-glucosepyrophosphorylase 1 (Ugp1) gene in flo8-1 and flo8-2,respectively. Complementation of the flo8-1 mutant restorednormal seed appearance by expressing full length codingsequence of Ugp1. RT-qPCR analysis revealed that Ugp1was ubiquitously expressed. Mutation caused the decreasedUGPase activity and affected the expression of most of genesassociated with starch biosynthesis. Meanwhile, western blotand enzyme activity analyses showed the comparability ofprotein levels and enzyme activity of most tested starchbiosynthesis related genes. Our results demonstrate thatUgp1 plays an important role for starch biosynthesis in riceendosperm.
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Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.
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Shaolu Zhao,Wuhua Long,Yihua Wang,Linglong Liu,Yunlong Wang,Mei Niu,Ming Zheng,Di Wang,Jianmin Wan 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3
Leaf-color mutants are ideal genetic materials for understanding the mechanism of chloroplast development and chlorophyll (Chl) biosynthesis. Here we isolated and identified a new leaf-color mutant of rice, named white-stripe leaf3 (wsl3), from a 60Co-irradiated mutant pool. The wsl3 mutant displayed a visible white-stripe leaf in both young seedlings and flag leaves of mature plant. Chl content in homozygous wsl3 mutant was approximately 47% of that in the wild type. Besides, chloroplast development in the mutant was severely arrested. By a map-based cloning strategy, the wsl3 gene was finely confined to a 50.8 kb region on chromosome 1. Moreover, a 9-bp deletion was identified in the genomic region of LOC_Os01g01920, which encodes an HD (histidine and aspartic acid) domaincontaining protein. Genetic complementation confirmed that LOC_Os01g01920 could recover the lesion of wsl3 mutation. Real-time PCR analyses showed that the expression levels of WSL3 were the highest in young and flag leaves among various tissues, and most of the genes associated with Chl biosynthesis were significantly down-regulated in the wsl3 mutant. Meanwhile, in contrast to many nuclear gene-encoded phage-type RNA polymerase(s) (NEP) transcribed genes were up-regulated, most of plastid-encoded bacterialtype RNA polymerase (PEP) transcribed genes were downregulated. These results demonstrated that the WSL3 gene, as an HD domain-containing protein, is involved in chl biosynthesis and chloroplast development in rice.
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