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Zhou, Li-Hua,Kang, Gum-Yong,Kim, Kwang Pyo John Wiley Sons, Ltd. 2009 Rapid communications in mass spectrometry Vol.23 No.15
<P>Application of matrix-assisted laser-desorption/ionization mass spectrometry (MALDI MS) to analysis and characterization of phosphopeptides in peptide mixtures may have a limitation, because of the lower ionizing efficiency of phosphopeptides than nonphosphorylated peptides in MALDI MS. In this work, a binary matrix that consists of two conventional matrices of 3-hydroxypicolinic acid (3-HPA) and α-cyano-4-hydroxycinnamic acid (CCA) was tested for phosphopeptide analysis. 3-HPA and CCA were found to be hot matrices, and 3-HPA not as good as CCA and 2,5-dihydroxybenzoic acid (DHB) for peptide analysis. However, the presence of 3-HPA in the CCA solution with a volume ratio of 1:1 could significantly enhance ion signals for phosphopeptides in both positive-ion and negative-ion detection modes compared with the use of pure CCA or DHB, the most common phosphopeptide matrices. Higher signal intensities of phosphopeptides could be obtained with lower laser power using the binary matrix. Neutral loss of the phosphate group (−80 Da) and phosphoric acid (−98 Da) from the phosphorylated-residue-containing peptide ions with the binary matrix was decreased compared with CCA alone. In addition, since the crystal shape prepared with the binary matrix was more homogeneous than that prepared with DHB, searching for ‘sweet’ spots can be avoided. The sensitivity to detect singly or doubly phosphorylated peptides in peptide mixtures was higher than that obtained with pure CCA and as good as that obtained using DHB. We also used the binary matrix to detect the in-solution tryptic digest of the crude casein extracted from commercially available low fat milk sample, and found six phosphopeptides to match the digestion products of casein, based on mass-to-charge values and LIFT TOF-TOF spectra. Copyright © 2009 John Wiley & Sons, Ltd.</P>
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Zhou, Yi-Li,Zhang, Wei,Gao, Er-Li,Dai, Xuan-Xuan,Yang, Han,Zhang, Xiao-Hua,Wang, Ou-Chen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Background and Objective: The optimal resection extent for clinically unilateral papillary thyroid microcarcinoma (PTMC) remains controversial. The objective was to investigate risk factors associated with occult contralateral carcinoma, and put emphasis on the predictive value of preoperative BRAF mutation. Materials and Methods: 100 clinically unilateral PTMC patients all newly diagnosed, previously untreated were analyzed in a prospective cohort study. We assessed the T1799A BRAF mutation status in FNAB specimens obtained from all PTMC patients before undergoing total thyroidectomy (TT) and central lymph node dissection (CLND) for PTMC. Univariate and multivariate analyses were used to reveal the incidence of contralateral occult cancer, difference of risk factors and predictive value, with respect to the following variables: preoperative BRAF mutation status, age, gender, tumor size, multifocality of primary tumor, capsular invasion, presence of Hashimoto thyroiditis and central lymph node metastasis. Results: 20 of 100 patients (20%) had occult contralateral lobe carcinoma. On multi-variate analysis, preoperative BRAF mutation (p = 0.030, OR = 3.439) and multifocality of the primary tumor (p = 0.004, OR = 9.570) were independent predictive factors for occult contralateral PTMC presence. However, there were no significant differences between the presence of occult contralateral carcinomas and age, gender, tumor size, capsular invasion, Hashimoto thyroiditis and central lymph node metastasis. Conclusions: Total thyroidectomy, including the contralateral lobe, should be considered for the treatment of unilateral PTMC if preoperative BRAF mutation is positive and/or if the observed lesion presents as a multifocal tumor in the unilateral lobe.
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Li, Ai-Hua,Liu, Hong-Can,Xin, Yu-Hua,Kim, Song-Gun,Zhou, Yu-Guang International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.2
<P>A Gram-stain-positive, aerobic, non-spore-forming, short-rod-shaped bacterium, designated strain MP203<SUP>T</SUP>, was isolated from ice water of Midui Glacier in Tibet Autonomous Region, China. The strain was psychrotolerant, growing at 0–25 °C. 16S rRNA gene sequence analysis showed that strain MP203<SUP>T</SUP> was most similar to <I>Frigoribacterium faeni</I> NBRC 103066<SUP>T</SUP>, <I>Compostimonas suwonensis</I> KACC 13354<SUP>T</SUP>, <I>Frigoribacterium mesophilum</I> KCTC 19311<SUP>T</SUP>, <I>Marisediminicola antarctica</I> CCTCC AB 209077<SUP>T</SUP> and <I>Alpinimonas psychrophila</I> JCM 18951<SUP>T</SUP>, with similarities of 97.4, 97.2, 97.2, 97.1 and 97.1 %, respectively. The maximum-likelihood phylogenetic tree indicated that strain MP203<SUP>T</SUP> clustered with nine genera of the family <I>Microbacteriaceae</I>, namely <I>Frigoribacterium</I>, <I>Compostimonas</I>, <I>Marisediminicola</I>, <I>Alpinimonas</I>, <I>Frondihabitans</I>, <I>Clavibacter</I>, <I>Subtercola</I>, <I>Klugiella</I> and <I>Agreia</I><I>.</I> However, bootstrap analysis showed that there was no significance in the branching pattern of the linage comprising strain MP203<SUP>T</SUP> and any existing generic lineage of the family <I>Microbacteriaceae</I>. DNA–DNA hybridization results indicated levels of relatedness between strain MP203<SUP>T</SUP> and <I>Marisediminicola antarctica</I> CCTCC AB 209077<SUP>T</SUP>, <I>Frigoribacterium faeni</I> NBRC 103066<SUP>T</SUP>, <I>Frigoribacterium mesophilum</I> KCTC 19311<SUP>T</SUP>, <I>Compostimonas suwonensis</I> KACC 13354<SUP>T</SUP> and <I>Alpinimonas psychrophila</I> JCM 18951<SUP>T</SUP> were 25.8±7.3, 29.6±7.6, 19.7±6.7, 16.0±4.2 and 12.4±5.1 % (mean±<SMALL>sd</SMALL>), respectively. The G+C content of the genomic DNA was 64.1 mol%. Analysis of the cell-wall peptidoglycan revealed that the peptidoglycan structure of strain MP203<SUP>T</SUP> was B10 type with Gly[<SMALL>l</SMALL>-Hse]–<SMALL>d</SMALL>-Glu–<SMALL>d</SMALL>-DAB, containing 2, 4-diaminobutyric acid (DAB) as a diagnostic amino acid. The cell-wall sugars were rhamnose, ribose, mannose and glucose. The major fatty acids were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB> and anteiso A-C<SUB>15 : 1</SUB>. An unusual compound identified as anteiso-C<SUB>15 : 0</SUB>-DMA (1, 1-dimethoxy-anteiso-pentadecane) was also present in strain MP203<SUP>T</SUP>. The predominant menaquinone was MK-10. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unknown glycolipid and four unknown lipids were detected in the polar lipid extracts. As strain MP203<SUP>T</SUP> was distinguishable from phylogenetically related genera in the family <I>Microbacteriaceae</I> in terms of its physiological and chemotaxonomic characteristics and phylogenetic position, it was considered to represent a novel species of a new genus. Thus, the name <I>Glaciihabitans tibetensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Glaciihabitans tibetensis</I> is MP203<SUP>T</SUP> ( = CGMCC 1.12484<SUP>T</SUP> = KCTC 29148<SUP>T</SUP>).</P>
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Li, Jun-Qin,Xue, Hui,Zhou, Lan,Dong, Li-Hua,Wei, Da-Peng,Li, Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
Objective: The mechanism of action of fatty acid synthase (FASN) in drug tolerance of breast cancer cells with epithelial-mesenchymal transition (EMT) features was investigated. Methods: The breast cancer cell line MCF-7-MEK5 with stably occurring EMT and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) tolerance was used as the experimental model, whereas MCF-7 acted as the control. Tumour cells were implanted into nude mice for in vivo analysis, and cerulenin was used as a FASN inhibitor. RT-PCR, real-time quantitative PCR and Western blot were employed to detect the expression of FASN, TNFR-1, TNFR-2, Wnt-1, ${\beta}$-catenin and cytC at the RNA and protein levels. Results: Compared with MCF-7, TNFR-1 expression in MCF-7-MEK5 was slightly changed, TNFR-2 was decreased, and FASN, Wnt-1, ${\beta}$-catenin and cytC were increased. The expression of Wnt-1 and ${\beta}$-catenin in MCF-7-MEK5 decreased after cerulenin treatment, whereas cytC expression increased. Conclusions: The important function of FASN in the drug tolerance of breast cancer may be due to the following mechanisms: FASN downregulated TNFR-2 expression through lipid rafts to make the cells less sensitive to TNF-${\alpha}$, and simultaneously activated the Wnt-$1/{\beta}$-catenin signalling pathway. Thus, cytC expression increased, which provided cells with anti-apoptotic capacity and induced drug tolerance.
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A Wide Dynamic Range NUC Algorithm for IRCS Systems
Li-Hua Cai,Feng-Yun He,Song-Tao Chang,Zhou Li 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.12
Uniformity is a key feature of state-of-the-art infrared focal planed array (IRFPA) and infrared imaging system. Unlike traditional infrared telescope facility, a ground-based infrared radiant char- acteristics measurement system with an IRFPA not only provides a series of high signal-to-noise ratio (SNR) infrared image but also ensures the validity of radiant measurement data. Normally, a long integration time tends to produce a high SNR infrared image for infrared radiant charac- teristics radiometry system. In view of the variability of and uncertainty in the measured target's energy, the operation of switching the integration time and attenuators usually guarantees the gual- ity of the infrared radiation measurement data obtainted during the infrared radiant characteristics radiometry process. Non-uniformity correction (NUC) coecients in a given integration time are often applied to a specified integration time. If the integration time is switched, the SNR for the infrared imaging will degenerate rapidly. Considering the effect of the SNR for the infrared image and the infrared radiant characteristics radiometry above, we propose a-wide-dynamic-range NUC algorithm. In addition, this essasy derives and establishes the mathematical modal of the algorithm in detail. Then, we conduct verification experiments by using a ground-based MWIR(Mid-wave Infared) radiant characteristics radiometry system with an 400 mm aperture. The experimental results obtained using the proposed algorithm and the traditional algorithm for different integration time are compared. The statistical data shows that the average non-uniformity for the proposed algorithm decreased from 0.77% to 0.21% at 2.5 ms and from 1.33% to 0.26% at 5.5 ms. The testing results demonstrate that the usage of suggested algorithm can improve infrared imaging quality and radiation measurement accuracy.
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Tanshinone II-A Inhibits Angiogenesis through Down Regulation of COX-2 in Human Colorectal Cancer
Zhou, Li-Hong,Hu, Qiang,Sui, Hua,Ci, Shu-Jun,Wang, Yan,Liu, Xuan,Liu, Ning-Ning,Yin, Pei-Hao,Qin, Jian-Min,Li, Qi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.
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Yong-Li Zhou,Xue-Wen Xie,Mei-Rong Xu,Jin-Ping Zang,Ling-Hua Zhu,Jian-Long Xu,Zhi-Kang Li 한국식물학회 2012 Journal of Plant Biology Vol.55 No.2
The genetic components responsible for the qualitative and quantitative resistance of rice to three Chinese races (C2, C4, and C5) of Xanthomonas oryzae pv. oryzae (Xoo)were investigated at the seedling and adult stages in two successive years in set of Lemont/Teqing cross introgression lines (ILs) in a Teqing background, to create a complete linkage map using 160 well-distributed SSR markers. Teqing was resistant to C2 and C4, but moderately susceptible to C5,whereas Lemont was susceptible to all three races. Highly significant correlations were detected among the resistance to different races at different developmental stages. A major gene (Xa4), 14 main-effect QTLs (M-QTLs), and 18 epistatic QTLs were identified in the two developmental stages over 2 years,and were largely responsible for the segregation of resistance in the ILs. In 2007, the Lemont alleles at all loci in the seedling stage, except QBbr10 to C4, increased lesion length (LL) or decreased resistance. The Teqing allele at the Xa4 locus acted as a resistance gene against C2 and C4, but acted as a M-QTL when its resistance was overcome by the virulent race C5. MQTLs showed a degree of race specificity and had a cumulative effect on resistance. Most M-QTLs (94%) consistently expressed resistance to the same race at the seedling and adult stages, indicating that a high degree of genetic overlap exists between Xoo resistance at both developmental stages in rice. Among the digenic interactions, most co-introgressed Lemont alleles at the two epistatic loci lead to significantly smaller LL with all three races, compared to other types of interacting alleles at both development stages. The results indicate that a high level of resistance may be achieved by the cumulative effect of multiple M-QTLs, including the residual effects of “defeated” major resistance genes and the epistatic effects of co-introgression from diverse susceptible varieties.
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Lo, Li,Belt, Simon T.,Lattaud, Julie,Friedrich, Tobias,Zeeden, Christian,Schouten, Stefan,Smik, Lukas,Timmermann, Axel,Cabedo-Sanz, Patricia,Huang, Jyh-Jaan,Zhou, Liping,Ou, Tsong-Hua,Chang, Yuan-Pin Elsevier 2018 Earth and planetary science letters Vol.488 No.-
<P><B>Abstract</B></P> <P>Recent reduction in high-latitude sea ice extent demonstrates that sea ice is highly sensitive to external and internal radiative forcings. In order to better understand sea ice system responses to external orbital forcing and internal oscillations on orbital timescales, here we reconstruct changes in sea ice extent and summer sea surface temperature (SSST) over the past 130,000 yrs in the central Okhotsk Sea. We applied novel organic geochemical proxies of sea ice (IP<SUB>25</SUB>), SSST ( TEX L 86 ) and open water marine productivity (a tri-unsaturated highly branched isoprenoid and biogenic opal) to marine sediment core MD01-2414 (53°11.77′N, 149°34.80′E, water depth 1123 m). To complement the proxy data, we also carried out transient Earth system model simulations and sensitivity tests to identify contributions of different climatic forcing factors. Our results show that the central Okhotsk Sea was ice-free during Marine Isotope Stage (MIS) 5e and the early-mid Holocene, but experienced variable sea ice cover during MIS 2–4, consistent with intervals of relatively high and low SSST, respectively. Our data also show that the sea ice extent was governed by precession-dominated insolation changes during intervals of atmospheric CO<SUB>2</SUB> concentrations ranging from 190 to 260 ppm. However, the proxy record and the model simulation data show that the central Okhotsk Sea was near ice-free regardless of insolation forcing throughout the penultimate interglacial, and during the Holocene, when atmospheric CO<SUB>2</SUB> was above ∼260 ppm. Past sea ice conditions in the central Okhotsk Sea were therefore strongly modulated by both orbital-driven insolation and CO<SUB>2</SUB>-induced radiative forcing during the past glacial/interglacial cycle.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The first orbital timescale proxy-model sea ice-sea surface temperature records from the northwestern subarctic Pacific Ocean. </LI> <LI> Strong precession forcing controlled and sea ice variations are modulated by greenhouse gas radiative forcing. </LI> <LI> Sea ice remained free in the central Okhotsk Sea during MIS 5e due to high greenhouse gas radiative forcing. </LI> </UL> </P>
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( Jiang Li Juan ),( Wen Juan Wu ),( Hai Wu ),( Son Sik Ryang ),( Jian Zhou ),( Wei Wu ),( Tao Li ),( Jian Guo ),( Hong Hai Wang ),( Shui Hua Lu ),( Yao Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.
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