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Estradiol enhances alternative activation of macrophages in white adipose tissue
( Jeong Min Choe ),( Kyeongdae Kim ),( Soomin Myoung ),( Dong-hoon Kim ),( Tak Kim ),( Jae-hoon Choi ),( Hyun Tae Park ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: Purpose - Our aim is to investigate how estradiol affects adipose tissue immune milieu and determine whether it improves glucose homeostasis in the long-term absence of estrogen. 방법: Materials and Methods – Female C57BL/6 mice were fed either normal chow (NCD) or 60% high-fat diets (HFD) ad libitum for 8 weeks after sham-operation (sham) or ovariectomy (OVX). Male C57BL/6 mice were used as controls. Female mice underwent long-term estrogen deficiency were administered 25 or 100/ug/kg/day of 17beta-estradiol (E2) subcutaneously via implanted ALZET mini-osmotic pump and sacrificed 2 weeks later. Snice E2 replacement reduced food intake, subjects treated with vehicle were subdivided with two groups as food intake: ad libitum and pair feeding. For detecting immune milieu of visceral adipose tissue and glucose homeostasis, flow cytometry measurements, oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed, respectively. 결과: Results – E2 replacement reduced body weight and calorie intake compared with group received HFDs ad libitum. We classified ATMs into 2 groups: M1 (CD11c+CD206-) and M2 (CD11c-CD206+) macrophages. E2 replacement increased M2 ATMs compared with control group regardless of food control. To elucidate underlying mechanisms for increased ATMs in E2 group, we performed Ki67 and adoptive transfer of GFP labeled monocytes. It is identified that GFP positive M2 ATMs is detected in visceral adipose tissue after E2 administration in OVX mice. Next, to determine whether estrogen improves glucose metabolism, GTT and ITT were performed. Compared to pair-fed group, 25 or 100 ug/kg/day of E2-treated groups did not improved glucose metabolism regardless of E2 concentration. 결론: Conclusions - Our results suggest E2 increases M2 ATMs by inducing peripheral monocytes recruitment to visceral adipose tissue and does not improve glucose metabolism under long-term estrogen deficiency.