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Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
Kotasová Hana,Capandová Michaela,Pelková Vendula,Dumková Jana,Koledová Zuzana,Remšík Ján,Souček Karel,Garlíková Zuzana,Sedláková Veronika,Rabata Anas,Vaňhara Petr,Moráň Lukáš,Pečinka Lukáš,Porokh Volo 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5
BACKGROUND The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. METHODS By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. RESULTS These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. CONCLUSION Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
Yen, Nguyen Hai,Thanh, Pham Thi,Koledov, Victor V.,Kamantsev, Alexander P.,Mashirov, Alexey V.,Thanh, Tran Dang,Hau, Kieu Xuan,Yu, Seong Cho,Dan, Nguyen Huy IEEE 2018 IEEE transactions on magnetics Vol.54 No.6
<P>In this paper, we investigated the influence of annealing conditions on magnetic properties, magnetocaloric effect, and critical parameters of Ni<SUB>50</SUB>Mn<SUB>50−<I>x</I></SUB>Sn<SUB><I>x</I></SUB> ( <TEX>$x = 13$</TEX>, 13.5, and 14) alloy ribbons prepared by using the melt-spinning method. The ribbons were annealed at 1123 K for various times. A martensitic–austenitic (M–A) structural phase transformation was observed on both the as-quenched and annealed samples. Temperature of the M–A phase transition ( <TEX>$T_{M-A}$</TEX>) of the ribbons can be regulated in room temperature region by changing the annealing time. Maximum positive and negative magnetic entropy changes, <TEX>$\vert \Delta S_{m}\vert _{\mathrm {max}}$</TEX>, larger than 3 and 1 <TEX>$\text {J}\cdot \text {kg}^{-1}\cdot \text {K}^{-1}$</TEX>, respectively, were achieved on the Ni<SUB>50</SUB>Mn<SUB>37</SUB>Sn<SUB>13</SUB> sample after annealing for 0.5 h. Critical parameters were determined to elucidate magnetic orders in the alloy. The obtained parameters are very close to those of the mean field theory of long-range ferromagnetic orders.</P>