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- 저자 : Kilcrease, J. P. (검색결과 2 건)
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Han, J. Y.,Chung, J.,Kim, J.,Seo, E. Y.,Kilcrease, J. P.,Bauchan, G. R.,Lim, S.,Hammond, J.,Lim, H. S. Springer Science + Business Media 2016 Virus genes Vol.52 No.4
<P>In 2014, we performed a nationwide survey in Korean radish fields to investigate the distribution and variability of Turnip mosaic virus (TuMV). Brassica rapa ssp. pekinensis sap-inoculated with three isolates of TuMV from infected radish tissue showed different symptom severities, whereas symptoms in Raphanus sativus were similar for each isolate. The helper component-protease (HC-Pro) genes of each isolate were sequenced, and phylogenetic analysis showed that the three Korean isolates were clustered into the basal-BR group. The HC-Pro proteins of these isolates were tested for their RNA silencing suppressor (VSR) activity and subcellular localization in Nicotiana benthamiana. A VSR assay by co-agroinfiltration of HC-Pro with soluble-modified GFP (smGFP) showed that HC-Pro of isolate R007 and R041 showed stronger VSR activity than R065. The HC-Pros showed 98.25 % amino acid identity, and weak VSR isolate (R065) has a single variant residue in the C-terminal domain associated with protease activity and self-interaction compared to isolates with strong VSR activity. Formation of large subcellular aggregates of GFP: HC-Pro fusion proteins in N. benthamiana was only observed for HC-Pro from isolates with strong VSR activity, suggesting that R065 'weak' HC-Pro may have diminished self-association; substitution of the variant C-terminal residue largely reversed the HC-Pro aggregation and silencing suppressor characteristics. The lack of correlation between VSR efficiency and induction of systemic necrosis (SN) suggests that differences in viral accumulation due to HC-Pro are not responsible for SN.</P>
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2015 Nationwide Survey Revealed Barley stripe mosaic virus in Korean Barley Fields
Lim, Hyo-Jin,Seo, Eun-Young,Kim, Hyun-Seung,Kim, Jung-Kyu,Park, Chan-Hwan,Gong, Jun-Su,Kim, Ik-hyun,Han, Sang-huk,Kilcrease, James P.,Furuya, Naruto FACULTY OF AGRIC PUBLICATIONS-KYUSHU UNIV 2016 Journal of the Faculty of Agriculture, Kyushu Univ Vol.61 No.1
<P>A seed transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed transmitted viruses, causing diseases which may spread. Barley cultivation in South Korea is expanding nationwide due to the increasing popularity of health foods, so both production and quality of barley grain is important. Although Barley stripe mosaic virus (BSMV) has been reported previously as a small percentage of all barley viruses present in Korea, increases in imports of barley seed may lead to increased occurrence of this seed transmitted virus. We therefore investigated the virus status of barley crops around Iksan and Wanju. On several newly cultivated barley farms, we observed areas showing symptoms typical of BSMV, and confirmed BSMV infection in 24% of the samples examined. In order to understand the occurrence and seed transmission of Korean BSMV isolates, we examined sequence variation within the Triple Gene Block proteins, and subcellular localization of two of these proteins. The newly identified Korean BSMV isolates show low sequence variability and high sequence homology to previously reported US isolates. With these results, we expect to confirm distribution of barley viruses and possible emerging viruses, which will serve as base line data to document virus prevention and control measures.</P>
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