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      • Haemolytic activity and reactive oxygen species production of four harmful algal bloom species

        Cho, Kichul,Kasaoka, Takuya,Ueno, Mikinori,Basti, Leila,Yamasaki, Yasuhiro,Kim, Daekyung,Oda, Tatsuya Taylor Francis 2017 European journal of phycology Vol.52 No.3

        <P>Based on haemolytic activity and reactive oxygen species (ROS) production of Chattonella marina, Chattonella antiqua, Heterocapsa circularisquama, Alexandrium tamiyavanichii and Karenia mikimotoi, the species were categorized into four types. (1) H. circularisquama: haemolytic activity was detected in both cell suspension and cell-free culture supernatant, but with greater activity in cell suspension than in the supernatant suggesting the presence of both cell surface and secreted haemolytic agents. (2) A. tamiyavanichii: equal haemolytic activities were detected in both the cell suspension and cell-free culture supernatant suggesting the presence of only secreted haemolytic agents. (3) K. mikimotoi: haemolytic activity was detected only in the cell suspension, indicating haemolytic agents occur only on the cell surface. (4) C. marina and C. antiqua: no significant haemolytic activity was detected in either cell suspension or cell-free culture supernatant, but high ROS were detected in the cell suspensions. Heterocapsa circularisquama and K. mikimotoi showed lethal effects on rotifers (Brachionus plicatilis), whereas A. tamiyavanichii, C. marina and C. antiqua had no effect. Our results suggest that H. circularisquama, K. mikimotoi and A. tamiyavanichii produce haemolytic agents with distinct characteristics, whereas C. marina and C. antiqua have an extremely potent ability to produce ROS.</P>

      • SCOPUSKCI등재

        Suppressed Fat Accumulation in Rats Fed a Histidine-Enriched Diet

        Endo, Michiko,Kasaoka, Seiichi,Takizawa, Miki,Goto, Kiyoko,Nakajima, Shigeru,Moon, Soo-Kyung,Kim, In-Soo,Jeong, Bo-Young,Nakamura, Soichiro The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.1

        The effect on body fat accumulation on male Wistar rats undergoing continuous feeding with a histidine-enriched diet was investigated. Five-week-age rats were assigned to two groups and were fed either the control diet (purified diet AIN-$76^{TM}$) or the histidine-enriched diet containing 3% histidine for 28 days. It was observed that both adipose tissue masses in retroperitoneal and epididymal areas of rats fed histidine-enriched diet significantly decreased (p<0.05) compared to those of control rats, while there was no significant difference in the food efficiency ratio between them. The blood levels of histidine derivatives of 3-methylhistidine and carnosine were significantly (p<0.05) increased in the rats fed a histidine-enriched diet, whereas there were no significant different between the histidine-enriched diet and control groups in the general amino acid distribution. Our results demonstrate that a histidine-enriched diet suppresses body fat accumulation in rats.

      • KCI등재

        Suppressed Fat Accumulation in Rats Fed a Histidine-Enriched Diet

        Michiko Endo,Seiichi Kasaoka,Miki Takizawa,Kiyoko Goto,Shigeru Nakajima,Soo-Kyung Moon,In-Soo Kim,Bo-Young Jeong,Soichiro Nakamura 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.1

        The effect on body fat accumulation on male Wistar rats undergoing continuous feeding with a histidine-enriched diet was investigated. Five-week-age rats were assigned to two groups and were fed either the control diet (purified diet AIN-76™) or the histidine-enriched diet containing 3% histidine for 28 days. It was observed that both adipose tissue masses in retroperitoneal and epididymal areas of rats fed histidine-enriched diet significantly decreased (p<0.05) compared to those of control rats, while there was no significant difference in the food efficiency ratio between them. The blood levels of histidine derivatives of 3-methylhistidine and carnosine were significantly (p<0.05) increased in the rats fed a histidine-enriched diet, whereas there were no significant different between the histidine-enriched diet and control groups in the general amino acid distribution. Our results demonstrate that a histidine-enriched diet suppresses body fat accumulation in rats.

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