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        Mitochondrial sequence based characterization and morphometric assessment of Diara buffalo population

        Singh Karan Veer,Purohit Hitesh,Singh Ramesh Kumar 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.7

        Objective: The present study is aimed at phenotypic characterization and mitochondrial d-loop analysis of indigenous “Diara” buffalo population, which are mostly confined to the villages on the South and North Gangetic marshy plains in the Bihar state of India. These buffaloes are well adapted and are best suited for ploughing and puddling the wet fields meant for paddy cultivation. Methods: Biometric data on 172 buffaloes were collected using a standard flexible tape measure. Animals are medium in size; the typical morphometric features are long head with a broad forehead and moderately long and erect ears. Genomic DNA was isolated from unrelated animals. The mtDNA d‐loop 358‐bp sequence data was generated and compared with 338 sequences belonging to riverine and swamp buffaloes. Results: Based on the mitochondrial d-loop analysis the Diara buffaloes were grouped along with the haplotypes reported for riverine buffalo. Sequence analysis revealed the presence of 7 mitochondrial D loop haplotypes with haplotype diversity of 0.9643. Five of the haplotypes were shared with established swamp breeds and with Buffalo population of Orissa in India. Conclusion: Morphometric analyses clearly shows distinguishing features like long and broad forehead which may be useful in identification. The germplasm of Diara buffalo is much adapted to the marshy banks of river Ganga and its tributaries. It constitutes a valuable genetic resource which needs to be conserved on priority basis. Objective: The present study is aimed at phenotypic characterization and mitochondrial d-loop analysis of indigenous “Diara” buffalo population, which are mostly confined to the villages on the South and North Gangetic marshy plains in the Bihar state of India. These buffaloes are well adapted and are best suited for ploughing and puddling the wet fields meant for paddy cultivation.Methods: Biometric data on 172 buffaloes were collected using a standard flexible tape measure. Animals are medium in size; the typical morphometric features are long head with a broad forehead and moderately long and erect ears. Genomic DNA was isolated from unrelated animals. The mtDNA d‐loop 358‐bp sequence data was generated and compared with 338 sequences belonging to riverine and swamp buffaloes.Results: Based on the mitochondrial d-loop analysis the Diara buffaloes were grouped along with the haplotypes reported for riverine buffalo. Sequence analysis revealed the presence of 7 mitochondrial D loop haplotypes with haplotype diversity of 0.9643. Five of the haplotypes were shared with established swamp breeds and with Buffalo population of Orissa in India.Conclusion: Morphometric analyses clearly shows distinguishing features like long and broad forehead which may be useful in identification. The germplasm of Diara buffalo is much adapted to the marshy banks of river Ganga and its tributaries. It constitutes a valuable genetic resource which needs to be conserved on priority basis.

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        Recent trends and challenges of surface electromyography in prosthetic applications

        Drishti Yadav,Karan Veer 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.3

        Surface electromyography (sEMG) meets extensive applications in the field of prosthesis in the current period. The effectivenessof sEMG in prosthesis applications has been verified by numerous revolutionary developments and extensive researchattempts. A large volume of research and literature works have explored and validated the vast use of these signals in prosthesesas an assistive technology. The objective of this paper is to conduct a systematic review and offer a detailed overviewof the work record in the prosthesis and myoelectric interfaces framework. This review utilized a systematic search strategyto identify published articles discussing the state-of-the-art applications of sEMG in prostheses (including upper limbprosthesis and lower limb prostheses). Relevant studies were identified using electronic databases such as PubMed, IEEEExplore, SCOPUS, ScienceDirect, Google Scholar and Web of Science. Out of 3791 studies retrieved from the databases,188 articles were found to be potentially relevant (after screening of abstracts and application of inclusion–exclusion criteria)and included in this review. This review presents an investigative analysis of sEMG-based prosthetic applications to assist thereaders in making further advancements in this field. It also discusses the fundamental advantages and disadvantages of usingsEMG in prosthetic applications. It also includes some important guidelines to follow in order to improve the performanceof sEMG-based prosthesis. The findings of this study support the widespread use of sEMG in prosthetics. It is concludedthat sEMG-based prosthesis technology, still in its sprouting phase, requires significant explorations for further development. Supplementary investigations are necessary in the direction of making a seamless mechanism of biomechatronics forsEMG-based prosthesis by cohesive efforts of robotic researchers and biomedical engineers.

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