http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Kanjanapangka, Julie (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Li, Feng,Yoshizawa, Janice M.,Kim, Kyoung-Mee,Kanjanapangka, Julie,Grogan, Tristan R.,Wang, Xiaoyan,Elashoff, David E.,Ishikawa, Shigeo,Chia, David,Liao, Wei,Akin, David,Yan, Xinmin,Lee, Min-Sun,Choi, American Association for Clinical Chemistry, Inc. 2018 Clinical chemistry Vol.64 No.10
<P><B>BACKGROUND:</B></P><P>Biomarkers are needed for noninvasive early detection of gastric cancer (GC). We investigated salivary extracellular RNA (exRNA) biomarkers as potential clinical evaluation tools for GC.</P><P><B>METHODS:</B></P><P>Unstimulated whole saliva samples were prospectively collected from 294 individuals (163 GC and 131 non-GC patients) who underwent endoscopic evaluation at the Samsung Medical Center in Korea. Salivary transcriptomes of 63 GC and 31 non-GC patients were profiled, and mRNA biomarker candidates were verified with reverse transcription quantitative real-time PCR (RT-qPCR). In parallel, microRNA (miRNA) biomarkers were profiled and verified with saliva samples from 10 GC and 10 non-GC patients. Candidate biomarkers were validated with RT-qPCR in an independent cohort of 100/100 saliva samples from GC and non-GC patients. Validated individual markers were configured into a best performance panel.</P><P><B>RESULTS:</B></P><P>We identified 30 mRNA and 15 miRNA candidates whose expression pattern associated with the presence of GC. Among them, 12 mRNA and 6 miRNA candidates were verified with the discovery cohort by RT-qPCR and further validated with the independent cohort (n = 200). The configured biomarker panel consisted of 3 mRNAs (<I>SPINK7</I>, <I>PPL</I>, and <I>SEMA4B</I>) and 2 miRNAs (<I>MIR140-5p</I> and <I>MIR301a</I>), which were all significantly down-regulated in the GC group, and yielded an area under the ROC curve (AUC) of 0.81 (95% CI, 0.72–0.89). When combined with demographic factors, the AUC of the biomarker panel reached 0.87 (95% CI, 0.80–0.93).</P><P><B>CONCLUSIONS:</B></P><P>We have discovered and validated a panel of salivary exRNA biomarkers with credible clinical performance for the detection of GC. Our study demonstrates the potential utility of salivary exRNA biomarkers in screening and risk assessment for GC.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
