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RISS 인기검색어
- 검색키워드
- 저자 : Kalpana Mody (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Kalpana Mody,Yasmin Khambhaty,B. Jha 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6
The phenotypic and carrageenolytic features of a novel halo tolerant marine bacterium, isolated from decayed red algal samples collected along the west coast of India were studied. This gram-negative strain was identified as Pseudomonas elongata (MTCC 5261) syn. Microbulbifer elongatus comb. nov according to its morphological, physiological and molecular characterization. The extracellular κ-carrageenase was purified 106.54-fold by a combination of ammonium sulfate precipitation (40~60%) and successive gel filtration chromatography. The purified protein fraction yielded significantly high activity of 426.19 units/mg protein and migrated as a single band on a sodium dodecyl sulfate polyacrylamide gel electrophoresis with a molecular weight of ~128 kDa. For κ-carrageenase activity, optimum temperature was 40℃ whereas two pH optima i.e. 5.6 and 7.7 were observed. For κ-carrageenan, the enzyme gave a Km value of 6.66 mg/mL and a Vmax value of 4 μmol/min/mg when the reaction was carried out at 40℃ and pH 5.6. Isolated κ-carrageenase could successfully generate protoplasts of Kappaphycus alvarezii. This is the first report on the production of κ-carrageenase by this bacterium isolated from west coast of India. Molecular mass and various characteristics showed that the carrageenase from P. elongata was much different from those previously reported.
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Gopalakrishnan Menon,Kalpana Mody,Jitendra Keshri 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6
A haloalkalitolerant xylanase-producing Bacillus pumilus strain, GESF1 was isolated from an experimental salt farm of CSMCRI. Birch wood xylan and xylose induced maximum xylanase production with considerable activity seen in wheat straw and no activity at all with caboxymethyl cellulose (CMC). A three step purification yielded 21.21-fold purification with a specific activity of 112.42 U/mg protein (unit expressed as μmole of xylose released per min). Xylanase produced showed an optimum activity at pH 8.0, with approximately 50 and 30% relative activity at a pH 6.0 and 10.0, respectively. The temperature optimum was 40°C and kinetic properties such as Km and Vmax were 5.3 mg/mL and 0.42 μmol/min/mL (6593.4μmol/min/mg protein). Xylanase activity (160 ~ 120%)was considerably enhanced in 2.5 to 7.5% NaCl with 87and 73% retention of activity in 10 and 15% of NaCl. Enzyme activity was enhanced by Ca2+, Mn2+, Mg2+, and Na+ but strongly inhibited by heavy metals such as Hg2+,Fe3+, Cu2+, Cd2+, and Zn2+. Organic reagents such as β-Mercaptoethanol enhanced xylanase activity whereas EDTA strongly inhibited its activity. Xylanase, purified from the Bacillus pumilus strain, GESF1 could have potential biotechnological applications.
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Khambhaty, Yasmin,Mody, Kalpana,Jha, B. Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6
The phenotypic and carrageenolytic features of a novel halo tolerant marine bacterium, isolated from decayed red algal samples collected along the west coast of India were studied. This gram-negative strain was identified as Pseudomonas elongata (MTCC 5261) syn. Microbulbifer elongatus comb. nov according to its morphological, physiological and molecular characterization. The extracellular k-carrageenase was purified 106.54-fold by a combination of ammonium sulfate precipitation $(40{\sim}60%)$ and successive gel filtration chromatography. The purified protein fraction yielded significantly high activity of 426.19 units/mg protein and migrated as a single band on a sodium dodecyl sulfate polyacrylamide gel electrophoresis with a molecular weight of ${\sim}128 kDa$. For k-carrageenase activity, optimum temperature was $40^{\circ}C$ whereas two pH optima i.e. 5.6 and 7.7 were observed. For k-carrageenan, the enzyme gave a $K_m$ value of 6.66 mg/mL and a $V_{max}$ value of $4{\mu}mol/min/mg$ when the reaction was carried out at $40^{\circ}C$ and pH 5.6. lsolated k-carrageenase could successfully generate protoplasts of Kappaphycus alvarezii. This is the first report on the production of k-carrageenase by this bacterium isolated from west coast of India. Molecular mass and various characteristics showed that the carrageenase from P. elongata was much different from those previously reported.
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Yasmin Khambhaty,Bhavanath Jha,Kalpana Mody 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
A marine, gram positive, aerobic, spore forming, and non flagellated bacterium which degrades low melting point (LMP) -agarose was isolated from the west coast of India and identified as Bacillus megaterium based on its mor-phological, biochemical, and molecular characterization. This bacterium produced clear haloes or zone of clearance on agar containing plates which was a clear indication of its agarolytic property. The extracellular agarase thus ob-tained was purified 8.8 and 78 fold from the culture supernatant by ammonium sulfate precipitation and gel filtration, respectively. Molecular mass by gel filtration and SDS-PAGE gave values of 15 and 12 kDa, respectively. The opti-mum temperature and pH for maximum agarase activity were 40C and 6.6. The activity of agarase was drastically reduced by addition of metal ions in the assay system. This agarase, gave a Km and Vmax value of 4 mg/mL and 2.75 μmol/min/mg. The isolation of protoplast from agarophyte like Gelidiella acerosa using indegenous agarase is re-ported for the first time.
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