Branched N-glycans과 세포 부착, 신호전달과 암 바이오마커와 치료를 위한 임상 응용에서의 branched N-glycans의 영향

( Taniguchi,Naoyuki ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 생화학분자생물학회 소식 Vol.31 No.4

Global Society and Capability Approach

Taniguchi, Julius R. 밝은사회연구소 2002 밝은社會硏究 Vol.23 No.1

Nutritional condition in the dry period is related to the incidence of postpartum subclinical endometritis in dairy cattle

Taniguchi Asako,Nishikawa Tatsuya,Morita Yasuhiro 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: Endometritis is a major disease, that causes infertility in cattle, and is usually categorized as clinical or subclinical endometritis (SCE). The nutritional condition during the dry period is important for recovery after the last stage of the lactation period, and for postpartum production and reproduction. This study aimed to clarify the relationship between nutritional and metabolic characteristics in the dry period, and the risk of postpartum SCE. Methods: Multiparous Holstein dairy cows (n = 25, raised in a tied stall) were used. Endometrial cytological analysis was performed around 30 days post-partum, with 5% to 14% polymorphonuclear (PMN) as a cut-off point to define SCE. Serum levels of glucose, non-esterified fatty acids, β-hydroxybutyric acid (BHBA), blood urea nitrogen, total cholesterol, aspartate aminotransferase, γ-glutamyl transpeptidase, calcium, phosphorus, and magnesium were measured in the cows at the dry period to evaluate energy status, protein metabolism, and mineral metabolism. Results: The incidence of SCE in the cows was 60.0% (n = 15/25) and the mean PMN% in postpartum cows diagnosed as SCE was 8.05%±2.6%. Overall, 17 and 8 samples were collected from the cows in the far-off and close-up periods, respectively. The serum concentration of BHBA in the far-off period and serum glucose concentration in the closeup period were correlated with postpartum PMN% (r = 0.62, p<0.01; r = –0.74, p<0.05, respectively). Serum levels of calcium and magnesium in the dry period were associated with the incidence of postpartum SCE (healthy vs SCE cows, p<0.05). Conclusion: Blood levels of glucose, BHBA, calcium, and magnesium in dry periods could be useful parameters for predicting the risk of postpartum SCE. The present study also suggests that management in the close-up period is essential for promoting recovery from calving fatigue. Objective: Endometritis is a major disease, that causes infertility in cattle, and is usually categorized as clinical or subclinical endometritis (SCE). The nutritional condition during the dry period is important for recovery after the last stage of the lactation period, and for postpartum production and reproduction. This study aimed to clarify the relationship between nutritional and metabolic characteristics in the dry period, and the risk of postpartum SCE.Methods: Multiparous Holstein dairy cows (n = 25, raised in a tied stall) were used. Endometrial cytological analysis was performed around 30 days post-partum, with 5% to 14% polymorphonuclear (PMN) as a cut-off point to define SCE. Serum levels of glucose, non-esterified fatty acids, β-hydroxybutyric acid (BHBA), blood urea nitrogen, total cholesterol, aspartate aminotransferase, γ-glutamyl transpeptidase, calcium, phosphorus, and magnesium were measured in the cows at the dry period to evaluate energy status, protein metabolism, and mineral metabolism.Results: The incidence of SCE in the cows was 60.0% (n = 15/25) and the mean PMN% in postpartum cows diagnosed as SCE was 8.05%±2.6%. Overall, 17 and 8 samples were collected from the cows in the far-off and close-up periods, respectively. The serum concentration of BHBA in the far-off period and serum glucose concentration in the closeup period were correlated with postpartum PMN% (r = 0.62, p<0.01; r = –0.74, p<0.05, respectively). Serum levels of calcium and magnesium in the dry period were associated with the incidence of postpartum SCE (healthy vs SCE cows, p<0.05).Conclusion: Blood levels of glucose, BHBA, calcium, and magnesium in dry periods could be useful parameters for predicting the risk of postpartum SCE. The present study also suggests that management in the close-up period is essential for promoting recovery from calving fatigue.

Non-existence of localized travelling waves with non-zero speed in single reaction-diffusion equations

Taniguchi, Masaharu,Ni, Wei-Ming,Kim, Yong Jung American Institute of Mathematical Sciences 2013 Discrete and continuous dynamical systems Vol.33 No.8

LYMANα EMITTERS BEYOND REDSHIFT 5: THE DAWN OF GALAXY FORMATION

TANIGUCHI YOSHIAKI,SHIOYA YASUHIRO,AJIKI MASARU,FUJITA SHINOBU S.,NAGAO TOHRU,MURAYAMA TAKASHI The Korean Astronomical Society 2003 Journal of The Korean Astronomical Society Vol.36 No.3

The 8m class telescopes in the ground-based optical astronomy together with help from the ultra-sharp eye of the Hubble Space Telescope have enabled us to observe forming galaxies beyond red shift z = 5. In particular, more than twenty Ly$\alpha$-emitting galaxies have already been found at z > 5. These findings provide us with useful hints to investigate how galaxies formed and then evolved in the early universe. Further, detailed analysis of Ly$\alpha$ emission line profiles are useful in exploring the nature of the intergalactic medium because the trailing edge of cosmic reionization could be close to z $\~$ 6 -7, at which forming galaxies have been found recently. We also discuss the importance of superwinds from forming galaxies at high redshift, which has an intimate relationship between galaxies and the intergalactic medium. We then give a review of early cosmic star formation history based on recent progress in searching for Ly$\alpha$-emitting young galaxies beyond red shift 5.

5-Aminolevulinic acid improves chicken sperm motility

Taniguchi Shin,Zhu Zhendong,Matsuzaki Mei,Tsudzuki Masaoki,Maeda Teruo 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: This study investigated the effects of 5-aminolevulinic acid (5-ALA) on the motility parameters, mitochondrial membrane depolarization, and ATP levels in chicken sperm. Methods: The pooled semen from Barred Plymouth Rock males was used. In the first experiment, the semen was diluted 4-times with phosphate-buffered saline (PBS (-)) containing various concentrations (0, 0.01, 0.05, and 0.1 mM) of 5-ALA, and then the sperm motility parameters after incubation were evaluated by computer-assisted sperm analysis (CASA). In the second experiment, the semen was diluted 4-times with PBS (-) containing 0.05 mM 5-ALA, and then sperm mitochondrial membrane depolarization and ATP levels after 1.5 h of incubation were analyzed with the MitoPT® JC-1 Assay and ATP Assay kits, respectively. In the third experiment, the semen was removed from the seminal plasma and resuspended with the mediums of PBS (-), PBS (-) supplemented with CaCl2 and MgCl2 (PBS (+)) + 5-ALA, PBS (+) + caffeine, and PBS (+) + caffeine + 5-ALA. Then, the sperm motility parameters after incubation were evaluated by CASA. In the last experiment, the semen was treated with the mediums of PBS (-), PBS (-) + 5-ALA, 5.7% glucose, 5.7% glucose + 5-ALA after removing the seminal plasma, and then the sperm motility parameters were evaluated by CASA. Results: The addition of 0.05 mM 5-ALA significantly increased the chicken sperm motility, progressive motility, linearity, average path velocity, curvilinear velocity, straight-line velocity, and the wobble. The sperm mitochondrial membrane depolarization was also increased by the 5-ALA treatment. The 5-ALA treatment decreased the sperm ATP levels. Both the caffeine treatment and glucose treatment decreased the sperm motility during incubation period. Conclusion: 5-ALA might increase sperm mitochondrial membrane depolarization to utilize the ATP for enhancing sperm movement. Objective: This study investigated the effects of 5-aminolevulinic acid (5-ALA) on the motility parameters, mitochondrial membrane depolarization, and ATP levels in chicken sperm.Methods: The pooled semen from Barred Plymouth Rock males was used. In the first experiment, the semen was diluted 4-times with phosphate-buffered saline (PBS (-)) containing various concentrations (0, 0.01, 0.05, and 0.1 mM) of 5-ALA, and then the sperm motility parameters after incubation were evaluated by computer-assisted sperm analysis (CASA). In the second experiment, the semen was diluted 4-times with PBS (-) containing 0.05 mM 5-ALA, and then sperm mitochondrial membrane depolarization and ATP levels after 1.5 h of incubation were analyzed with the MitoPT^® JC-1 Assay and ATP Assay kits, respectively. In the third experiment, the semen was removed from the seminal plasma and resuspended with the mediums of PBS (-), PBS (-) supplemented with CaCl2 and MgCl2 (PBS (+)) + 5-ALA, PBS (+) + caffeine, and PBS (+) + caffeine + 5-ALA. Then, the sperm motility parameters after incubation were evaluated by CASA. In the last experiment, the semen was treated with the mediums of PBS (-), PBS (-) + 5-ALA, 5.7% glucose, 5.7% glucose + 5-ALA after removing the seminal plasma, and then the sperm motility parameters were evaluated by CASA.Results: The addition of 0.05 mM 5-ALA significantly increased the chicken sperm motility, progressive motility, linearity, average path velocity, curvilinear velocity, straight-line velocity, and the wobble. The sperm mitochondrial membrane depolarization was also increased by the 5-ALA treatment. The 5-ALA treatment decreased the sperm ATP levels. Both the caffeine treatment and glucose treatment decreased the sperm motility during incubation period.Conclusion: 5-ALA might increase sperm mitochondrial membrane depolarization to utilize the ATP for enhancing sperm movement.

Iodide Suppression of Major Histocompatibility Class I Gene Expression in Thyroid Cells Involves Enhancer A and the Transcription Factor NF-kB

Taniguchi, Shin-Ichi,Shong, Minho,Giuliani, Cesidio,Napolitano, Giorgio,Saji, Motoyasu,Montani, Valeria,Suzuki, Koichi,Singer, Dinah S.,Kohn, Leonard D. 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Increased MHC class I expression on thyrodytes is evident in ATD (4, 21) and has been suggested to be an important causal factor in the development or perpetuation of disease expression (3). The hypothesis has been offered that the elevations result in abnormal presentation of thyroid antigens to immune cells, thereby breaking tolerance, and that this elicits the clonal expansion of autoreactive T cells associated with disease expression (3).

Numerical simulation of the crack propagation behavior in 3D elastic body

Taniguchi, Takeo,Miyaji, Akihiko,Suetsugu, Takeshi,Matsunaga, Shohgo Techno-Press 1994 Structural Engineering and Mechanics, An Int'l Jou Vol.2 No.3

The purpose of this investigation is to propose a numerical simulation method of the crack propagation behavior in 3-dimensionl elastic body. The simulation method is based on the displacement-type finite element method, and the linear fracture theory is introduced. The results from the proposed method are compared with those from the structural experiments, and the good coincidences between them are shown in this paper. At the same time, 2-dimensional analysis is also done, and the results are compared with those obtained from 3-dimensional analysis and the structural experiments.

Experimental Characterization of Dynamic Tensile Strength in Unidirectional Carbon/Epoxy Composites

Taniguchi, Norihiko,Nishiwaki, Tsuyoshi,Kawada, Hiroyuki The Korean Society for Composite Materials 2008 Advanced composite materials Vol.17 No.2

This study aims to characterize the dynamic tensile strength of unidirectional carbon/epoxy composites. Two different carbon/epoxy composite systems, the unidirectional T700S/2500 and TR50S/modified epoxy, are tested at the static condition and the strain rate of $100\;s^{-1}$. A high-strain-rate test was performed using a tension-type split Hopkinson bar technique with a specific fixture for specimen. The experimental results demonstrated that both tensile strength increase with strain rate, while the fracture behaviors are quite different. By the use of the rosette analysis and the strain transformation equations, the strain rate effects of material principal directions on tensile strength are investigated. It is experimentally found that the shear strain rate produces the more significant contribution to strain rate effect on dynamic tensile strength. An empirical failure criterion for characterizing the dynamic tensile strength was proposed based on the Hash-in's failure criterion. Although the proposed criterion is just the empirical formula, it is in better agreement with the experimental data and quite simple.

THE HST COSMOS PROJECT: CONTRIBUTION FROM THE SUBARU TELESCOPE

TANIGUCHI YOSHIAKI,SCOVILLE N. Z.,SANDERS D. B.,MOBASHER B.,AUSSEL H.,CAPAK P.,AJIKI M.,MURAYAMA T.,MIYAZAK S.,KOMIYAMA Y.,SHIOYA Y.,NAGAO T. The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2

The Cosmic Evolution Survey (COSMOS) is a Hubble Space Telescope (HST) treasury project. The COSMOS aims to perform a 2 square degree imaging survey of an equatorial field in I(F814W) band, using the Advanced Camera for Surveys (ACS). Such a wide field survey, combined with ground-based photometric and spectroscopic data, is essential to understand the interplay between large scale structure, evolution and formation of galaxies and dark matter. In 2004, we have obtained high-quality, broad band images of the COSMOS field (B, V, r', i', and z') using Suprime-Cam on the Subaru Telescope, and we have started our new optical multi-band program, COSMOS-21 in 2005. Here, we present a brief summary of the current status of the COSMOS project together with contributions from the Subaru Telescope. Our future Subaru program, COSMOS-21, is also discussed briefly.