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RISS 인기검색어
- 검색키워드
- 저자 : Juan Contreras-Ramos (검색결과 4 건)
- 무료
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Luz M. Ramos-Ponce,Mireille Vega,Edith Colunga-Urbina,Georgina C. Sandoval-Fabián,Nagamani Balagurusamy,Juan Carlos Contreras-Esquivel,Francisco J. Rodriguez-Gonzalez 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3
A colorimetric method for quantitative measurement of free amino groups of water soluble chitin derivatives is described. The method utilizes genipin as a natural and specific reagent for determining the concentration of free amino groups in samples of water soluble chitin derivatives. The blue color adduct (complex) formed during genipin reaction with free amino groups was measured at about 589 nm and Beer-Lambert’s law obeyed over the concentration range of 50 to 300 mg/L. Parameters of analytical conditions were considered and kept constant during the experimental procedure. Highly acetylated water soluble chitin derivatives can be differentiated from water soluble chitosan using this genipin method. The colorimetric method with genipin was proved to be a rapid and efficient technique to determine the free amino groups in water soluble chitin derivatives. This method can also be applied for the detection of the enzymatic activity of chitindeacetylase.
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Rapid physicochemical characterization of innovative fucoidan/fructan powders by ATR-FTIR
Espinosa-Velazquez, Gerardo,Ramos-de-la-Pena, Ana Mayela,Montanez, Julio,Contreras-Esquivel, Juan Carlos 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
Functional food has been highly demanded lately because of its benefits in counteracting diseases. Fucoidan and agave fructan are ingredients that enhance the growth of beneficial bacteria in the gut (prebiotics). This mixture has great potential to develop innovative products but it has never been explored before. Because of fucoidan is more expensive than agave fructan, the innovative proposed mixture is vulnerable to adulteration. This research was aimed to assess the accuracy of Fourier transform infrared spectroscopy with attenuated total reflectance (ATR-FTIR) coupled with chemometrics to identify and predict concentration of both polysaccharides in powder mixtures (0-100%). Absorption bands at 1240-1255 and $836-840cm^{-1}$ were attributed to fucoidan and a strong peak at ${\sim}936cm^{-1}$ confirmed the fructan presence. Peak areas were best fitted into linear models ($R^2_{adj}{\geq}0.92$, $RMSE{\leq}3.54%$). This achievement may be useful to certificate ingredients contained in fucoidan-fructan mixtures, preventing adulteration.
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Wilma Tixi-Verdugo,Juan Contreras-Ramos,Gloria Sicilia-Argumedo,Michael S. German,Cristina Fernandez-Mejia 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.3
During maturation, pancreatic islets achieve their full capacity to secrete insulin in response to glucose, undergo morphological changes in which alpha-cells decrease and beta-cell mass increases, and they acquire the normal alpha- and beta-cell proportion changes that are important for islet functions later in life. In rodents, the first week of postweaning is critical for islet maturation. Multiple studies have documented the detrimental effects of several conditions on pancreatic maturation; however, few studies have addressed the use of pharmacological agents to enhance islet maturation. Biotin might have a potential action on islet maturation. Pharmacological concentrations of biotin have been found to modify islet morphology and function. In a previous study, we found that mice fed a biotin-supplemented diet for 8 weeks after weaning showed an increase in basal and glucose stimulated insulin secretion, enlarged islet size, and modified islet structure. In the present study, we investigated the effect of biotin on maturation features during the first week postweaning. Female BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 1 week after weaning. Compared with the control, biotin-supplemented mice showed an increase in pancreatic islet number and area in addition to an augmented proportion of beta-cells in the islet. These effects were related to an increase in beta-cell proliferation. No differences were found in insulin secretion, blood glucose concentrations, or serum insulin levels. These results indicate that biotin supplementation is capable of affecting beta-cell proliferation and might be a therapeutic agent for establishing strategies for regenerative medicine.
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Rapid physicochemical characterization of innovative fucoidan/ fructan powders by ATR–FTIR
Gerardo Espinosa-Vela´zquez,Ana Mayela Ramos-de-la-Pen˜a,Julio Montanez,Juan Carlos Contreras-Esquivel 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
Functional food has been highly demanded lately because of its benefits in counteracting diseases. Fucoidan and agave fructan are ingredients that enhance the growth of beneficial bacteria in the gut (prebiotics). This mixture has great potential to develop innovative products but it has never been explored before. Because of fucoidan is more expensive than agave fructan, the innovative proposed mixture is vulnerable to adulteration. This research was aimed to assess the accuracy of Fourier transform infrared spectroscopy with attenuated total reflectance (ATR–FTIR) coupled with chemometrics to identify and predict concentration of both polysaccharides in powder mixtures (0–100%). Absorption bands at 1240–1255 and 836–840 cm-1 were attributed to fucoidan and a strong peak at * 936 cm-1 confirmed the fructan presence. Peak areas were best fitted into linear models (R2 adj C 0.92, RMSE B 3.54%). This achievement may be useful to certificate ingredients contained in fucoidan– fructan mixtures, preventing adulteration.
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