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        Functional Bioactivities of Soluble Seed Proteins from Two Leguminous Seeds

        Taiwo Scholes Adewole,Boluwatife Bolanle Dudu,Johnson Olaleye Oladele,Oyedotun Moses Oyeleke,Adenike Kuku 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.2

        Storage proteins from Sphenostylis stenocarpa and Phaseolus lunatus were fractionated, and their in vitro bioactivitieswere investigated. Albumin, globulin, prolamin, and glutelin constituents of the respective seeds were successivelyfractionated using the modified Osborne method. Phenylmethylsulfonyl fluoride (1 mM) was used as a protease inhibitor. The antioxidant, anti-inflammatory, and acetylcholinesterase-inhibitory activities of the protein fractions were evaluatedusing different appropriate techniques. Globulin was the predominant fraction, with a yield of 43.21±0.01% and 48.19±0.03% for S. stenocarpa and P. lunatus, respectively, whereas prolamin was not detected in both seeds. The protein fractionmarkedly scavenges hydroxyl radicals, nitric oxide radicals, and 2,2-diphenyl-1-picryldydrazyl radicals with concomitanthigh free radical-reducing power. Albumin and globulin fractions elicited the highest acetylcholinesterase-inhibitory potentialof 48.75% and 49.75%, respectively, indicating their great application potential in managing neurodegenerative diseases. In this study, the albumin, globulin, and glutelin fractions of these underutilized legumes showed great analepticbioactivities, which could be utilized as health-promoting dietary supplements/products.

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