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RISS 인기검색어
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- 저자 : Jiwen Zhao (검색결과 3 건)
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Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6
Huang Jiwen,Zhao Ayong,He Daqian,Wu Xiao,Yan Huaxiang,Zhu Lihui 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1
The proteins carried by the extracellular vesicles of Lactobacillus salivarius SNK-6 (LsEVs) were identified to provide a foundation for further explorations of the probiotic activities of L. salivarius SNK-6. LsEVs were isolated from the culture media of L. salivarius SNK-6 and morphological analysis was conducted by scanning electron microscopy. Subsequent transmission electron microscopy and nanoparticle tracking analysis were performed to assess the morphology and particle size of the LsEVs. In addition, the protein composition of LsEVs was analyzed using silver staining and protein mass spectrometry. Finally, internalization of the identified LsEVs was confirmed using a confocal microscope, and enzyme-linked immunosorbent assay was employed to analyze the levels of inflammatory cytokines in LPS-challenged RAW264.7 cells. The results revealed that the membraneenclosed LsEVs were spherical, with diameters ranging from 100–250 nm. The LsEVs with diameters of 111–256 nm contained the greatest amount of cargo. In total, 320 proteins (10–38 kD) were identified in the LsEVs and included anti-inflammatory molecules, such as PrtP proteinase, cochaperones, and elongation factor Tu, as well as some proteins involved in glycolysis/gluconeogenesis, such as fructose-1,6-bisphosphate aldolase. Enrichment analysis showed these proteins to be related to the terms “metabolic pathway,” “ribosome,” “glycolysis/gluconeogenesis,” “carbohydrate metabolism,” and “amino acid metabolism.” Furthermore, the LsEVs were internalized by host liver cells and can regulate inflammation. These findings confirm that LsEVs contain various functional proteins that play important roles in energy metabolism, signal transduction, and biosynthesis.
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Fast FCS-MPC-Based SVPWM Method to Reduce Switching States of Multilevel Cascaded H-Bridge STATCOMs
Xiuqin Wang,Jiwen Zhao,Qunjing Wang,Guoli Li,Maosong Zhang 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1
Finite control set model-predictive control (FCS-MPC) has received increasing attentions due to its outstanding dynamicperformance. It is being widely used in power converters and multilevel inverters. However, FCS-MPC requires a lot ofcalculations, especially for multilevel-cascaded H-bridge (CHB) static synchronous compensators (STATCOMs), since it has totake account of all the feasible voltage vectors of inverters. Hence, an improved five-segment space vector pulse widthmodulation (SVPWM) method based on the non-orthogonal static reference frames is proposed. The proposed SVPWM methodhas a lower number of switching states and requires fewer computations than the conventional method. As a result, it makesFCS-MPC more efficient for multilevel cascaded H-bridge STATCOMs. The partial cost function is adopted to sequentiallysolve for the reference current and capacitor voltage. The proposed FCS-MPC method can reduce the calculation burden of theFCS-MPC strategy, and reduce both the switching frequency and power losses. Simulation and experimental results validate theexcellent performance of the proposed method when compared with the conventional approach.
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Fast FCS-MPC-Based SVPWM Method to Reduce Switching States of Multilevel Cascaded H-Bridge STATCOMs
Wang, Xiuqin,Zhao, Jiwen,Wang, Qunjing,Li, Guoli,Zhang, Maosong The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1
Finite control set model-predictive control (FCS-MPC) has received increasing attentions due to its outstanding dynamic performance. It is being widely used in power converters and multilevel inverters. However, FCS-MPC requires a lot of calculations, especially for multilevel-cascaded H-bridge (CHB) static synchronous compensators (STATCOMs), since it has to take account of all the feasible voltage vectors of inverters. Hence, an improved five-segment space vector pulse width modulation (SVPWM) method based on the non-orthogonal static reference frames is proposed. The proposed SVPWM method has a lower number of switching states and requires fewer computations than the conventional method. As a result, it makes FCS-MPC more efficient for multilevel cascaded H-bridge STATCOMs. The partial cost function is adopted to sequentially solve for the reference current and capacitor voltage. The proposed FCS-MPC method can reduce the calculation burden of the FCS-MPC strategy, and reduce both the switching frequency and power losses. Simulation and experimental results validate the excellent performance of the proposed method when compared with the conventional approach.
KCI
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