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      • The Review of New NDT Methods of Metal Material Fatigue Monitoring

        Jingwei Dong,Qijian Wu,Wenwen Jiang,Qi Xu 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.8

        Non-destructive testing (NDT) techniques have been employed in all kinds of fields successfully for many years. Newly developed and applied NDT methods of detecting and monitoring fatigue crack in metal material are reviewed in this paper. Some methods based on machine vision technique and image recognition techniques are introduced for at or near surface visible crack testing. As a newborn offspring of acoustic emission technique, electromagnetically induced acoustic emission methods also introduced for both visible and invisible fatigue cracks on metal structure. Nonlinear ultrasonic method and some other NDT methods presently available are mentioned, too. The principles, applied testing fields, data processing algorithms, advantages and disadvantages of these methods are discussed.

      • The Improved Neural Network Algorithm of License Plate Recognition

        Jingwei Dong,Meiting Sun,Gengrui Liang,Kui Jin 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.5

        With high definition cameras are widely used in urban traffic monitoring, the resolution of vehicle and traffic monitoring image rises. As the main component of intelligent traffic system, the vehicle license plate recognition technique faces new challenges and some new demands are put forward. Aiming at the problems of cutting down the time delay and eliminating characters outside of vehicle license plate, a set of license plate recognition algorithm is developed in this paper. The process of recognition in this paper is divided into four parts: image preprocessing, license plate location, license plate segmentation and character recognition. In license plate location, an accelerating matching method based on particle image correlation method is employed. In character recognition, an improved BP neural network algorithm is employed. The principle, procedure and simulation of the algorithm is described in details. High definition images with 2 mega pixels are used as the input of simulation.

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        MicroRNA-181a-5p Curbs Osteogenic Differentiation and Bone Formation Partially Through Impairing Runx1-Dependent Inhibition of AIF-1 Transcription

        Jingwei Liu,Daming Dong,Xueying Chang 대한내분비학회 2023 Endocrinology and metabolism Vol.38 No.1

        Background: Evidence has revealed the involvement of microRNAs (miRNAs) in modulating osteogenic differentiation, implying the promise of miRNA-based therapies for treating osteoporosis. This study investigated whether miR-181a-5p influences osteogenic differentiation and bone formation and aimed to establish the mechanisms in depth. Methods: Clinical serum samples were obtained from osteoporosis patients, and MC3T3-E1 cells were treated with osteogenic induction medium (OIM) to induce osteogenic differentiation. miR-181a-5p-, Runt-related transcription factor 1 (Runx1)-, and/or allograft inflammatory factor-1 (AIF-1)-associated oligonucleotides or vectors were transfected into MC3T3-E1 cells to explore their function in relation to the number of calcified nodules, alkaline phosphatase (ALP) staining and activity, expression levels of osteogenesis-related proteins, and apoptosis. Luciferase activity, RNA immunoprecipitation, and chromatin immunoprecipitation assays were employed to validate the binding relationship between miR-181a-5p and Runx1, and the transcriptional regulatory relationship between Runx1 and AIF-1. Ovariectomy (OVX)-induced mice were injected with a miR-181a-5p antagonist for in vivo verification. Results: miR-181a-5p was highly expressed in the serum of osteoporosis patients. OIM treatment decreased miR-181a-5p and AIF-1 expression, but promoted Runx1 expression in MC3T-E1 cells. Meanwhile, upregulated miR-181a-5p suppressed OIM-inducedincreases in calcified nodules, ALP content, and osteogenesis-related protein expression. Mechanically, miR-181a-5p targeted Runx1, which acted as a transcription factor to negatively modulate AIF-1 expression. Downregulated Runx1 suppressed the miR-181a-5p inhibitor-mediated promotion of osteogenic differentiation, and downregulated AIF-1 reversed the miR-181a-5p mimic-induced inhibition of osteogenic differentiation. Tail vein injection of a miR-181a-5p antagonist induced bone formation in OVX-induced osteoporotic mice. Conclusion: In conclusion, miR-181a-5p affects osteogenic differentiation and bone formation partially via the modulation of the Runx1/AIF-1 axis.

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