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Jin Myeon Park,Seong Eun Lee,Tae Jun Lim,Jae Seung Noh 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6
This study was carried out to investigate the effect of slurry composting and biofiltration (SCB) liquid manure application on shoot growth, fruit qualities and soil chemical properties in peach orchard. SCB liquid manure was fertigated ten times from April to October in SCB plot, whereas chemical fertilizer was treated two times as basal and additional fertilizers in control plot. The shoot growth, leaf nitrogen and potassium content, soil exchangeable K, fruit weight and yield were higher in SCB plot than in control. Soluble solid content and acidity, soil organic matter, soil available phosphate and soil exchangeable Mg showed no significant difference between treatments, and the leaf calcium and magnesium content were lower in SCB plot than in control. In conclusion, fertigating SCB liquid manure in peach orchard has positive effects on fruit weight and yield, and it is suggested that periodical soil testing is needed because of the possibility of K accumulation in SCB liquid manure treated soil when the orchard is fertigated based on the soil nitrogen content.
Park, Jong-Myeon,Kim, Minseok S.,Moon, Hui-Sung,Yoo, Chang Eun,Park, Donghyun,Kim, Yeon Jeong,Han, Kyung-Yeon,Lee, June-Young,Oh, Jin Ho,Kim, Sun Soo,Park, Woong-Yang,Lee, Won-Yong,Huh, Nam American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.8
<P>Full automation with high purity for circulating tumor cell (CTC) isolation has been regarded as a key goal to make CTC analysis a “bench-to-bedside” technology. Here, we have developed a novel centrifugal microfluidic platform that can isolate the rare cells from a large volume of whole blood. To isolate CTCs from whole blood, we introduce a disc device having the biggest sample capacity as well as manipulating blood cells for the first time. The fully automated disc platform could handle 5 mL of blood by designing the blood chamber having a triangular obstacle structure (TOS) with lateral direction. To guarantee high purity that enables molecular analysis with the rare cells, CTCs were bound to the microbeads covered with anti-EpCAM to discriminate density between CTCs and blood cells and the CTCs being heavier than blood cells were only settled under a density gradient medium (DGM) layer. To understand the movement of CTCs under centrifugal force, we performed computational fluid dynamics simulation and found that their major trajectories were the boundary walls of the DGM chamber, thereby optimizing the chamber design. After whole blood was inserted into the blood chamber of the disc platform, size- and density-amplified cancer cells were isolated within 78 min, with minimal contamination as much as approximately 12 leukocytes per milliliter. As a model of molecular analysis toward personalized cancer treatment, we performed epidermal growth factor receptor (EGFR) mutation analysis with HCC827 lung cancer cells and the isolated cells were then successfully detected for the mutation by PCR clamping and direct sequencing.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-8/ac403456t/production/images/medium/ac-2013-03456t_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac403456t'>ACS Electronic Supporting Info</A></P>
Park, Jin Myeon,Lee, Seong Eun,Lim, Tae Jun,Noh, Jae Seung 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6
This study was carried out to investigate the effect of slurry composting and biofiltration (SCB) liquid manure application on shoot growth, fruit qualities and soil chemical properties in peach orchard. SCB liquid manure was fertigated ten times from April to October in SCB plot, whereas chemical fertilizer was treated two times as basal and additional fertilizers in control plot. The shoot growth, leaf nitrogen and potassium content, soil exchangeable K, fruit weight and yield were higher in SCB plot than in control. Soluble solid content and acidity, soil organic matter, soil available phosphate and soil exchangeable Mg showed no significant difference between treatments, and the leaf calcium and magnesium content were lower in SCB plot than in control. In conclusion, fertigating SCB liquid manure in peach orchard has positive effects on fruit weight and yield, and it is suggested that periodical soil testing is needed because of the possibility of K accumulation in SCB liquid manure treated soil when the orchard is fertigated based on the soil nitrogen content.
무기질 및 유기질 비료 시용이 고추 수량과 토양화학성에 미치는 영향
박진면(Jin Myeon Park),이인복(In Bog Lee),강윤임(Yun Im Kang),황기성(Ki Sung Hwang) 한국원예학회 2009 원예과학기술지 Vol.27 No.1
고추밭에서 화학비료 및 퇴비의 14년간 연용 및 결제처리 후 토양화학성 및 수량 변화와 양분 흡수량을 조사한 결과는 다음과 같다. 토양화학성 변화 중 유기물함량은 퇴비 처리시 매년 1.11-1.17g·㎏<SUP>-1</SUP>씩 증가하였고 무퇴비구에서도 매년 0.51-0.55g·㎏<SUP>-1</SUP>씩 증가하였다. 유효인산함량은 매년 N₀P₀K₀ 처리에서 3.6㎎·㎏<SUP>-1</SUP>, NK+퇴비 처리에서 16.㎎·㎏<SUP>-1</SUP>, NPK+퇴비 처리에서 45.㎎·㎏<SUP>-1</SUP>씩 증가하였다. 치환성 칼륨함량은 N₀P₀K₀ 처리에서 0.22c㏖·㎏<SUP>-1</SUP>까지 감소하였으며 NPK 처리는 0.50-0.71cmol·㎏<SUP>-1</SUP>에서 안정되어 평형을 이루었다. 치환성 마그네슘함량은 NK+퇴비 및 N₀P₀K₀에서 낮 았다. 고추의 양분흡수량과 수량은 N₀P₀K₀<PK+퇴비<NK+퇴비<NPK=NP+퇴비≤NPK+퇴비 순으로 많아 질소 비료가 고추의 수량 및 생육에 크게 영향을 미치며 인산은 흡수량이 적어 인산 축적을 방지하기 위하여 인산 시비량을 줄 일 필요가 있다. This study was conducted to investigate the effects of mineral and organic fertilizer application on pepper (Capsicum annuum) yield and soil chemical properties for 14 years from 1994 to 2007. For the experiment, experimental plots were established on a sandy loam soil with six treatments, which consisted of no fertilization (N₀P₀K₀), N+P+K standard fertilization (NPK), N+P+K+compost (NPKC), P+K+compost (PKC), N+P+compost (NPC), and N+K+compost (NKC). During the 14-yr experimental period, organic matter contents in soil increased continuously at the rate of 1.11-1.17 g·㎏<SUP>-1</SUP>·yr<SUP>-1</SUP> in the treatments with compost applications and 0.51-0.55 g·㎏<SUP>-1</SUP>·yr<SUP>-1</SUP> in treatment with no compost application. The soil available phosphate in N0P0K0 treatment increased at the rate of 3.6 ㎎·㎏<SUP>-1</SUP>·yr<SUP>-1</SUP>, which was insignificant compared to those in compost applied treatments such as 16.1 ㎎·㎏<SUP>-1</SUP>·yr<SUP>-1</SUP> in NKC and 45.0 ㎎·㎏<SUP>-1</SUP>·yr<SUP>-1</SUP> in NPKC. As cultivation periods for pepper was extended, exchangeable potassium concentrations in soil tended to decline continuously. But, exchangeable potassium content in soil showed no further decline at the level of 0.22 c㏖·㎏<SUP>-1</SUP> in N₀P₀K₀ and 0.50-0.71 c㏖·㎏<SUP>-1</SUP> in NPK treatment. Fruit yield and the amount of nutrient absorbed by pepper increased in the following order: N₀P₀K₀ < PKC < NKC < NPK ≒ NPC < NPKC, indicating that hot pepper shows the greatest response to nitrogen. From the results of long-term mineral and organic fertilizer application, it is obvious that nitrogen is the important nutrient for production of pepper fruit. The amount of phosphate fertilization recommended currently for pepper should be reduced to avoid accumulation of phosphate in soil.
돈분 액비 관비가 오이의 생육 및 토양화학성에 미치는 영향
박진면(Jin Myeon Park),임태준(Tae Jun Lim),이성은(Seong Eun Lee),이인복(In Bok Lee) 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.2
본 연구는 시설 하우스 재배에서 돈분뇨 액비와 화학비료의 관비처리가 오이 생육, 무기성분 함량, 수량, 양분흡수량 및 이용률, 토양 화학성에 미치는 영향을 알아보기 위하여 수행되었다. 오이 재배 양식은 반촉성과 억제재배이며 시험 처리는 무비, 질소 기준으로 돈분뇨 액비 26 mg L<SUP>-1</SUP>, 52 mg L<SUP>-1</SUP> 및 화학비료 52 mg L<SUP>-1</SUP> 4처리를 하였다. 돈분뇨 액비와 화학비료 처리는 초장에 차이가 없으나 돈분뇨 액비 26 mg L<SUP>-1</SUP> 처리에서 줄기와 뿌리의 생체중과 건물중이 많았다. 잎의 칼슘함량은 돈분뇨 액비 처리에서 낮았으며 뿌리의 칼륨함량은 화학비료 52 mg L<SUP>-1</SUP> 처리에서 낮았다. 줄기의 칼슘함량은 화학비료 52 mg L<SUP>-1</SUP>에서 가장 낮았고 과실의 무기성분 함량은 처리간에 차이가 없었다. 돈분뇨 액비 및 화학비료를 처리에서 총수량은 반촉성재배에서 화학비료 52 mg L<SUP>-1</SUP> 처리가 적었으며 비상품과 수량은 돈분뇨 액비 처리에서 적었다. 양분 이용률은 질소 4.2~13.0%, 인산은 1.9~2.0%, 칼륨은 8.3~30.9%로 칼륨, 질소,인산 순이다. 토양 화학성 중 pH는 돈분뇨 액비 처리가 화학비료 처리보다 높았고 EC 및 질산태 질소함량은 화학비료 처리가 돈분뇨 액비 처리보다 많았다. 치환성 칼륨함량은 돈분뇨 액비 52 mg L<SUP>-1</SUP> 처리구가 높았으며 칼슘과 마그네슘은 처리간에 차이가 없었다. 결과적으로 오이 관비 재배에서 토양 검정에 의한 돈분뇨 액비 시용은 화학비료 질소와 칼리를 대체할 수 있을 것으로 판단되었다. This study was conducted to evaluate fertigation effects of pig slurry (PS) and chemical fertilizer (CF) in cucumber by investigating the growth and yield, nutrient content and uptake, nutrient use efficiency, and soil characteristics in greenhouse cultivation. The cropping patterns of cucumber were semi-forcing culture and retarding culture, and the experiment involves four treatments: No fertilizer (NF), 26 mg L<SUP>-1</SUP> and 52 mg L<SUP>-1</SUP> of N application by slurry composting biofiltration liquefied fertilizer (SCBLF), and 52 mg L<SUP>-1</SUP> of N treatment by chemical fertilizer. The difference on the plant height of cucumber between SCBLF and CF treatments was no significant, but fresh weight and dry weight of stem and root were higher in 26 mg L<SUP>-1</SUP> SCBLF treatment. The Ca content of the leaf was lower in the treatments of SCBLF and the K content of the root was lower in the 52 mg L<SUP>-1</SUP> CF treatment. The Ca content of the stem was lowest in the 52 mg L<SUP>-1</SUP> CF treatment and the mineral content of the fruit showed no significant difference. In case of semi-forcing culture, the gross yield was lower in the 52 mg L<SUP>-1</SUP> CF treatment and the yield of unmarketable fruit was lower in the SCBLF treatments. The nutrient use efficiency of cucumber is as follows: K (8.3 ~ 30.9%), N (4.2 ~ 13.0%), P (1.9 ~ 2.0%). The SCBLF treatments showed higher figure in the soil pH than that of the CF treatment, while EC and the content of NO₃-N were higher in the CF treatment than the former. The content of exchangeable K was higher in the plot treated with 52 mg L<SUP>-1</SUP> SCBLF, and there were no significant differences in the content of Ca and Mg between the treatments. In conclusion, it is suggested that the application of liquefied manure made from pig slurry may be able to replace the use of chemical fertilizer in nitrogen and potassium.