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Platelet Function Analyzer(PFA®)-100을 이용한 술전 출혈경향 평가 및 임상적 적용
Jihu han,도언록,김태섭,Chen Zhang,박대환 대한미용성형외과학회 2013 Archives of Aesthetic Plastic Surgery Vol.19 No.1
Routine preoperative tests such as BT/CT, PT/PTT and CBC, could not test abnormal hemostasis that take drugs and health functional food. We used platelet function analyzer (PFA®)-100, to evaluate preoperative bleeding tendencies. From November 2008 to February 2010, 306 surgical patients were tested preoperative PFA in our hospital. There are 2 tests in PFA®-100. The Pre Operative EPI (collagen/epinephrine) assay tests extrinsic platelet function, whereas the Pre Operative ADP (collagen/adenosine diphosphate) assay tests intrinsic platelet function. We divided normal and abnormal groups by the PFA®-100 assay tests. If either of results were abnormal in the two tests, the patient was divided to the abnormal group. 306 surgical patients were observed with hemorrhagic complications. All of the patients were divided normal and abnormal groups by the PFA®-100 test result. The normal group was made up of 286 (93.5 %) patients, the abnormal group was made up of 20 (6.5 %) patients. We observed each group hemorrhage complication including sever echymosis and hematoma, and analyzed each group complication rate. There were 9 (3.1 %) cases of complication in the normal group. There were 3 (15.0 %) cases of complication in the abnormal group. To evaluate preoperative bleeding tendency, PFA®-100 can be complementary examination with previous routine blood coagulation tests.
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Liu Jianbai,Wu Han,Yi Jiequn,Li Jihu,Cheng Yinjie,Cheng Yinjie,Sun Donglei,An Yuxing 한국곤충학회 2022 Entomological Research Vol.52 No.2
Chilo sacchariphagus is one of the most harmful pests of sugarcane, sorghum, corn, and other crops, in China and other countries and regions. In order to elucidate the molecular mechanisms involved in the sexually dimorphic development of C. sacchariphagus, transcriptome data of female and male adults were obtained. A total of 127,812,174 and 126,593,664 clean reads of males and females were arranged into 45,693 and 37,034 unigenes for males and females, respectively, 26,949 of which were annotated. Candidate genes involved in sexual development were identified and analysed. Statistical analysis revealed that 18,642 genes were differentially expressed in females and males, 9,307 of which were up-regulated in males and 9,335 of which were up-regulated in females. As indicated by GO classification, DEGs were mainly involved in cell part, cellular process and binding. KEGG enrichment analysis showed that 6,037 DEGs were assigned to 295 metabolic pathways. Based on annotation and transcriptome data, we identified twenty-two sex determining genes, of which Csactra2, Csacemc, Csacfru, Csacix, Csacovo, CsacdsxM,andCsacsxl showed a higher expression in males than that in females, while CsacgroX4, Csacfem, CsacgroX9, Csacmsl1, Csacmsl2, Csacmsl3, Csacotu, Csacvir, Csacrunt,andCsacdsxF were more highly expressed in female. In addition, Csactra2 and CsacgroX9 were enriched in Spliceosome, Notch signaling, and Wnt signaling pathways, respectively. And the pathways are crucial in regulating insect growth, differentiation and development. This transcriptome study provides rich and significant information regarding the genes involved in sex differentiation and determination, which would improve our understanding of the molecular mechanisms related to sex determination and be helpful for providing the basis for a wide spectrum of strategies to benefit pest control and prevention, and agriculture and public health
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Liu Jianbai,Yi Jiequn,Wu Han,Lu Yinglin,Mao Yongkai,Lin Mingjiang,Li Jihu,An Yuxing 한국곤충학회 2022 Entomological Research Vol.52 No.9
Chilo sacchariphagus Bojeris is one of the most dangerous pests of sugarcane. The larvae damage the seedlings and stems of sugarcane and also harm sorghum, corn and other crops, which causes great economic losses to the sugar industry every year. Transcriptome sequencing and expression profile analysis of cytochrome P450 monooxygenase (CYP), carboxylesterase and glutathione S-transferase (GST) were carried out, which could provide a basis for drug resistance monitoring and drug resistance management of the pest. Unigenes of C. sacchariphagus were obtained by using the Illumina HiSeqTM 4,000 platform as 150 bp paired-end reads. A total of 173,013 unigenes were obtained after data assembly and redundancy removal. 28,330 unigenes were annotated based on multiple public databases, and the number of unigenes annotated by NR database was the largest. According to the transcriptome analysis, 214 candidate detoxification enzyme genes were identified, including 44 GSTs,138CYPs, and 32 CarEs. Phylogenetic analysis showed that the CYPs were mainly clustered in CYP4, CYP6, CYP9 and CYP12 subfamilies; CarEs mainly include antennal CarEs, venom CarEs and CarEs NOTUM; while the GSTs cluster mainly contains subfamilies such as delta, omega, epsilon, theta, zeta and sigma. In this study, transcriptome information of C. sacchariphagus was obtained, and genes related to detoxification were identified, which could provide data and a basis for the further study of detoxification and host plant adaptation mechanism of C. sacchariphagus.
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