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        Changes in the volatile profi le of ‘Fantasia’ nectarines [ Prunus persica (L.) Batsch, var. nectarina] treated with an enhanced freshness formulation (EFF) containing hexanal

        Shanthanu Krishna Kumar,Tom Hern,David Liscombe,Gopinadhan Paliyath,J. Alan Sullivan,Jayasankar Subramanian 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.3

        Postharvest technologies play a key role in enhancing shelf life and maintaining quality characteristics of tender fruits, suchas nectarines ( Prunus persica [L.] Batsch var. nectarina). This research investigated the eff ects of an enhanced freshnessformulation (EFF), containing hexanal as the key ingredient, in enhancing the shelf life of fi eld grown nectarines and itseff ects on the composition of volatile organic compounds (VOCs). ‘Fantasia’ nectarine fruits at two commercial orchards inthe Niagara region were subject to preharvest sprays of 2% EFF (15 and 7 days preharvest) and stored at ambient conditions(25 °C) with volatile analyses conducted at 0, 3, 5, 7, and 11 days postharvest. The volatile analysis study indicated for thefi rst time that hexanal is naturally present in nectarine fruit. This was confi rmed by GC–MS analysis and validated withstandards. GC–EIMS analyses of targeted VOCs validated with standards indicated reduced levels of VOCs associated withfruit ripening and fruity odor, such as lactones, in hexanal-treated fruit, and an increased level of C6 compounds associatedwith unripe fruit. Principal component analysis biplot with scores and loadings of the fruit VOCs and quality parametersindicated a tight association between TSS and lactones, which was inversely associated with fi rmness, ground color, acetates,and ester compounds. Pearson correlation coeffi cients also indicated a very high positive correlation between hexanal andlevels of VOCs, such as 1-hexanol, E-2-hexanal, 3-hexanal, hexen-1-ol, and other C6 acetates. These fi ndings may indicatea delay in the ripening process caused by hexanal formulation, which may be associated with modulated expression of keyripening-related volatile pathways, therefore enhancing the shelf life and quality of nectarines.

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        Hexanal Vapor Induced Resistance against Major Postharvest Pathogens of Banana (Musa acuminata L.)

        Durgadevi Dhakshinamoorthy,Srivignesh Sundaresan,Arumukapravin Iyadurai,Kizhaeral Sevathapandian Subramanian,Gnanaguru Janaki Janavi,Gopinathan Paliyath,Jayasankar Subramanian 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.2

        Hexanal, a C-6 aldehyde has been implicated to have antimicrobial properties. Hence, this study was con- ducted to determine the antifungal activities of hexanal vapor against major postharvest pathogens of banana viz., Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The pathogens were cultured in vitro and exposed to hexanal vapor at 600, 800, 1,000 and 1,200 ppm. Mycelial growth of both fungal pathogens were inhibited completely at 800 ppm and the incidence of anthracnose and stem-end rot diseases reduced by 75.2% and 80.2%, respectively. The activities of peroxidase, polyphenol oxidase, phenylalanine am- monia-lyase and glucanase had transiently increased in hexanal vapor treated banana by 5 to 7 days and declined thereafter. Postharvest treatment of banana with hexanal vapor resulted in phospholipase D inhi- bition and also resulted in cell wall thickening of the treated fruit, which impeded the penetration of the pathogenic spores. This was further confirmed by scan- ning electron micrographs. The defense-related protein intermediaries had increased in hexanal vapor treated banana fruit, which suggests induced resistance against C. gloeosporioides and L. theobromae, via., the phen- ylpropanoid pathway which plays a significant role in hindering the pathogen quiescence. Delayed ripening due to inhibition of phospholipase D enzyme, inhibition of mycelial growth and induced systemic resistance by defense enzymes collectively contributed to the posthar- vest disease reduction and extended shelf life of fruit.

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