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- 저자 : Idris Siti Khadijah (검색결과 1 건)
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Idris Siti Khadijah,Ramli Bin Abdullah,Wan Embong Wan Khadijah,Mohammad Mijanur Rahman 한국통합생물학회 2013 Animal cells and systems Vol.17 No.3
Combination of different chemicals has been an effective method to activate oocytes. The objectives of this study were to evaluate the optimal: (1) concentration (Experiment 1) and duration (Experiment 2) of strontium chloride (SrCl2) in the presence of cytochalasin B (CB), (2) secondary activation agent [6-dimethylaminopurine (6-DMAP),cycloheximide (CHX) and CB] in combination with calcium ionophore (CaI) (Experiment 3) and (3) ethanol (EtOH)concentration in combination with 6-DMAP (Experiment 4). There were no significant (P0.05) differences in cleavage (86.8289.06%) and blastocyst (33.4246.83%) rates when oocytes were treated with various concentrations of SrCl2 (210 mM). Three-hour duration generally showed the highest cleavage (93.83%) and blastocyst rates (60.42%) compared to other durations. Comparing with various combinations, CaI6-DMAP showed the highest cleavage at all stages of development and significantly (PB0.05) higher from other combinations at 8-cell (50.58%),morula (30.29%) and blastocyst (23.28%) stages. When comparing the EtOH concentrations, 8% EtOH gave significantly (PB0.05) highest cleavage rates at 8-cell stage (39.70%), morula (21.50%) and blastocyst (13.86%) stages followed by 9% and 7% EtOH. In conclusion, 10 mM SrCl25 mg/ml CB in calcium-free Chatot Ziomek Bavister medium for 3-h incubation duration gave the highest cleavage rates of parthenotes mouse oocytes.
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