落羽松의 몇개 處理要因이 發根에 미친 效果에 關한 硏究

金英彩,鄭炫培 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

Experiments were conducted to investigate the rooting of cuttings of Japanese Yew (Taxodium distichum Rich.) as influenced by ages of cutting, preparation methods, and some treatments of cut surface. Cutting of 2-year-old twig rooted better than older cuttings when compared in terms of number of roots and root length. Cuttings prepared as single rod type showed better survival ratio, more roots, and longer root length than heel cuttings. Soaking the cut surface in water enhanced rooting of cuttings than heating treatment.

혈청이 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

유지창,정헌택,김유현,진효상,김신무,배현옥 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1999 Journal of biomedical laboratory sciences Vol.5 No.1

마우스 간 세포주인 BNL CL.2의 시험관내 배양에서 혈청과 IFN-γ가 세포주의 nitric oxide(NO) 생성과 세포 손상에 미치는 영향을 알아보기 위한 실험을 하였다. 혈청이 공급된 배양에서 IFN-γ에 의한 세포 생존율은 거의 변동이 없었으나, 혈청을 제거한 배양에서는 약 65%의 생존율이 유지되었으며, NO 생성 억제제인 N -monomethy-L-arginine (NMA)의 첨가는 농도 의존적으로 세포의 생존율을 감소시켰다. 혈청이 제거된 BNL CL.2세포주는 IFN-γ단독 처리에서도 NO 를 생성할 수 있었으며, IFN-γ와 lipopolysaccharide (LPS)의 복합 처리는 세포주의 NO 생성을 상승적으로 증가시켰다. 또한 protein tyrosine kinase (PTK) inhibitor인 herbimycin A와 genistein에 의해서 NO 생성이 억제되어 PTK의 활성이 혈청이 고갈된 BNL CL.2 세포에서 NO의 생성에 중요한 역할을 담당하고 있기 때문으로 판단된다. IFN-γ의 독성은 혈청을 제거시킬 때 NO 생성 억제제에 상승적으로 간 세포를 손상시키며, 이때 NO가 IFN-γ에 의해 유도된 손상을 어느 정도 억제시키는 것을 알 수 있었다. Nitric oxide (NO) plays an important role in immunologic defense, and influences upon the functioning of secretory tissues and cells. It also exhibits cytotoxic/cytostatic activity as one of major operating effectors of the cellular immunity system. We investigated the effects of serum on the cell damages and NO production in the mouse liver cell line BNL CL.2 to establish the role of NO. We observed that, when BNL CL.2 cells were cultured in serum-free medium, they were induced to cell damage by the stimulation of IFN-γalone or IFN-γplus LPS. Serumstarved cells showed large amount of nitrite accumulation and NO synthase (NOS) expression in response to IFN-γalone in dose-and time-dependent manners, but serum-supplied cells did not. The production of NO was blocked by protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin. These results suggest that the deprivation of serum in the BNL CL.2 cell culture medium might primed with the cells to produce NO when the cells are triggered by IFN-γ and the involvement of PTK signal transduction pathway in the expression of NOS gene in murine hepatocytes.

솔잎혹파리의 被害程度가 소나무의 結實에 미치는 影響 (Ⅱ) : 被害率과 毬果 및 種子形質과의 關係 Relationship between Infestation Rate and Several Characters of Cone and Seed

全尙根,鄭炫培,張仁和 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

This study was carried out to investigate the effects of infestation rate by the pine gall midge on several characters of cone and seed in Pinus densiflora Sieb. et Zucc. 'The results were summarized as follows: 1. 'The averages of cone characters were 31.59 ± 0.61 mm in length, 19.12 ± 0.28 mm in diameter, 6.06 ± 0.22 g in fresh weight, and 3.17 ± 0.12 g in dry weight. Most these characters have a tendency to decrease with increase of tree age and with increase in infestation rate. 2. The averages of seed characters were 4.94 ± 0.06 mm in length, 3.06 ± 0.08 mm in diameter, 1.83 ± 0.02 mm in thickness, and 8.33 ± 0.34 mg in weight. Most these characters have a tendency to decrease with the increase in infestation rate. 3. Most coefficients of correlation showed negative relationships between infestation rate and each character of cone and seed, but they were not significant statistically.

잣나무에 있어서 結實量이 直徑生長에 미치는 影響

全尙根,金英彩,鄭炫培 경희대학교 부설 식량자원개발연구소 1981 硏究論文集 Vol.2 No.-

This study was carried out to know the effect of cone production on diameter increment in Pinus koraiensis. For the cone production per tree, the number of one year-old immature cone and 2 year-old mature cone and the latter's weight were investigated, and for the diameter increment, width of annual ring and it's relative value to the radius were investigated for the 28 individuals of 45 year-old tree over a period of 4 years. And then the correlation between cone production and diameter increment were investigated. The results obtained are as follows: 1. A significant correlation between the number of immature cone (one year-old cone) and the width of annual ring was not recognizable. 2. A negative correlation between the number of mature cone and the width of annual ring was found significantly. 3. A large quantity of the mature cone production decreased not only the diameter increment in the year, but also effected the following year increment.

잣나무 苗木의 養苗法 改善에 關한 硏究報告 Ⅱ : 露天埋藏의 時期別差가 잣나무 幼苗의 生長에 미치는 影響 Effects of Open Ground Storage Period on Seedling Growth in Korean White Pine

金英彩,全尙根,鄭炫培 경희대학교 부설 식량자원개발연구소 1984 硏究論文集 Vol.5 No.-

This research was performed to estimate the seedling growth of Korean white pine based on open ground storage period to stimulate seed germination. The results are as follows: 1. The length of seedlings gradually increased as the storage period was delayed. The contribution rate to seedling growth by storage period was 23.84%. 2. The diameter was irregular, but larger with early open ground storage. The contribution rate to diameter by storage period was 38.25%. 3. The Seedling weight showed a tendency to be decreased with late seed storge. The contribution rate to weight growth by storage period was 18.41%.

1,2,3,4,6-penta-O-galloyl-beta-D-glucose up-regulates heme oxygenase-1 expression by stimulating Nrf2 nuclear translocation in an extracellular signal-regulated kinase-dependent manner in HepG2 cells.

Pae, Hyun-Ock,Oh, Gi-Su,Jeong, Sun-Oh,Jeong, Gil-Saeng,Lee, Bok-Soo,Choi, Byung-Min,Lee, Ho-Sub,Chung, Hun-Taeg WJG Press 2006 WORLD JOURNAL OF GASTROENTEROLOGY Vol.12 No.2

<P>To examine the potency of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) as a hepatic heme oxygenase-1 (HO-1) inducer and its regulation in HepG2 cells.</P>

Heme oxygenase-1 attenuates contact hypersensitivity induced by 2,4-dinitrofluorobenzene in mice.

Pae, Hyun-Ock,Ae Ha, Young,Chai, Kyu-Yun,Chung, Hun-Taeg Marcel Dekker 2008 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY Vol.30 No.2

<P>Heme oxygenase (HO)-1 may have an important role in the resolution of T cell-mediated inflammation. The authors elucidated the role of the anti-inflammatory HO-1 in the pathogenesis of skin inflammation, using a mouse contact hypersensitivity (CHS) induced by 2,4-dinitrofluorobenzene (DNFB). Ear swelling was induced by challenge with DNFB, accompanied by infiltration of inflammatory cells in the challenged ear skin. DNFB challenge induced low levels of HO-1 mRNA and protein expression. Ear swelling induced by DNFB challenge was significantly reduced by topical treatment with cobalt protoporphyrin IX (CoPP), a HO-1 inducer, but exaggerated by blockage of HO-1 activity with tin protoporphyrin IX (SnPP), a HO-1 inhibitor. Similarly, the number of infiltrated cells in DNFB-challenged ear skin were reduced by CoPP but increased by SnPP. Our findings suggest that HO-1 plays an important role in CHS and is an important pharmacological target for the treatment of CHS.</P>

Heme Oxygenase-1 : Its Therapeutic Roles in Inflammatory Diseases

Pae, Hyun-Ock,Chung, Hun-Taeg 대한면역학회 2009 Immune Network Vol.9 No.1

Heme oxygenase (HO)-1 is an inducible enzyme that catalyzes the first and rate-limiting step in the oxidative degradation of free heme into ferrous iron, carbon monoxide (CO), and biliverdin (BV), the latter being subsequently converted into bilirubin (BR). HO-1, once expressed during inflammation, forms high concentrations of its enzymatic by-products that can influence various biological events, and this expression is proven to be associated with the resolution of inflammation. The degradation of heme by HO-1 itself, the signaling actions of CO, the antioxidant properties of BV/BR, and the sequestration of ferrous iron by ferritin all concertedly contribute to the anti-inflammatory effects of HO-1. This review focuses on the anti-inflammatory mechanisms of HO-1 actions and its roles in inflammatory diseases.

A molecular cascade showing nitric oxide-heme oxygenase-1-vascular endothelial growth factor-interleukin-8 sequence in human endothelial cells.

Pae, Hyun-Ock,Oh, Gi-Su,Choi, Byung-Min,Kim, Young-Myeong,Chung, Hun-Taeg Association for the Study of Internal Secretions 2005 Endocrinology Vol.146 No.5

<P>Heme oxygenase (HO)-1 has been shown to be an important biological target of nitric oxide (NO). NO can induce HO-1 expression and IL-8 production, particularly, in endothelial cells. Interestingly, HO-1 tends to induce the production of vascular endothelial growth factor (VEGF) that is involved in endothelial IL-8 syntheses. Whether HO-1 expression by NO may provide a link with IL-8 or VEGF synthesis was investigated in human umbilical vein endothelial cells (HUVECs). The NO donor S-nitroso-N-acetyl-penicillamine (SNAP) dose-dependently increased IL-8 and VEGF productions and HO-1 expression in HUVECs. Transfection with either HO-1 small interfering RNA or HO-1 antisense oligodeoxynucleotide abrogated the ability of SNAP to induce HO-1 expression and IL-8 and VEGF productions. Both pharmacological induction and gene transfer of HO-1 directly induced IL-8 and VEGF productions. Anti-VEGF neutralizing antibody blocked SNAP-mediated IL-8 production and VEGF itself induced IL-8 production, whereas anti-IL-8 neutralizing antibody had no effect on VEGF production in SNAP-treated HUVECs. Neither anti-VEGF nor anti-IL-8 antibodies influenced SNAP-induced HO-1 expression. Moreover, neither VEGF nor IL-8 showed an additive effect on SNAP-induced HO-1 expression. HO-1 transfection had no significant effect on productions of other CXC chemokines, such as growth-related oncogen-alpha and epithelial neutrophil activation peptide-78. Taken together, these results provide a molecular cascade showing NO-HO-1-VEGF-IL-8 sequence in human endothelial cells.</P>

Integrative Survival Response Evoked by Heme Oxygenase-1 and Heme Metabolites

Pae, Hyun-Ock,Kim, Eun-Cheol,Chung, Hun-Taeg the Society for Free Radical Research Japan 2008 Journal of clinical biochemistry and nutrition Vol.42 No.3

<P>Heme oxygenase (HO) catalyzes the rate-limiting step in heme degradation to produce carbon monoxide (CO), iron, and biliverdin. Biliverdin is subsequently converted to bilirubin by its reductase, and iron is recycled for heme synthesis. The inducible HO isoform, HO-1, is involved in the protection of multiple tissues and organs. The mechanism of protective actions of HO-1 has not been completely elucidated, but recent evidence suggests that one or more of heme metabolites can mediate the protective effects of HO-1. Particularly, CO mimics the antioxidant, anti-inflammatory, anti-apoptotic and antiproliferative actions of HO-1. Many of these effects of CO depend on the production of cyclic guanosine monophosphate (cGMP), and the modulation of mitogen-activated protein kinase (MAPK) pathways. The transcription factors, including nuclear factor E2-related factor-2 (Nrf2), and their upstream kinases, including MAPK pathway, play an important regulatory role in HO-1 expression by dietary antioxidants and drugs. This review attempts to concisely summarize the molecular and biochemical characteristics of HO-1, with a discussion on the mechanisms of signal transduction and gene regulation that mediate the induction of HO-1 by dietary antioxidants and drugs. In addition, the cytoprotective roles of HO-1 shall be discussed from the perspective of each of the metabolic by-products.</P>