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Xiaolu Li,Xiaomin Xue,Ling Zhang,Xueming Wang,Huchang Wang 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.5
Adding ring-stiff eners is an eff ective strategy for strengthening CHS tubular joints. However, research on the capacity of ring-stiff ened CHS tube-gusset joints remains limited. In this study, we performed experimental and numerical analyses to derive static strength equations for this joint. For convenience, we simplifi ed the complex joint model into a ring plate to CHS joint, which consisted of a CHS tube and a ring-stiff ener. First, we tested fi ve specimens to obtain failure mode and ultimate strength. Second, a fi nite element model was established and validated by the experimental results. Then, we used the verifi ed numerical method to simulate the behavior of the ring plate to CHS joint. A wide range of geometric parameters was considered, and the relevant design guide was assessed. Finally, nonlinear analysis was performed, and a design formula was proposed. Good agreement was achieved between the proposed design formula and the results of experimental and numerical analyses.
Human Intersectin 2 (ITSN2) binds to Eps8 protein and enhances its degradation
( Xiao Feng Ding ),( Zijian Yang ),( Fang Liang Zhou ),( Xiang Huchang ),( Zhou Chang Luo ),( Zhi Cheng He ),( Qian Liu ),( Hong Li ),( Feng Yan ),( Fang Mei Wang ),( Shuang Lin Xiang ),( Jian Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.3
Participates in actin remodeling through Rac and receptor endocytosis via Rab5. Here, we used yeast two-hybrid system with Eps8 as bait to screen a human brain cDNA library. ITSN2 was identified as the novel binding factor of Eps8. The interaction between ITSN2 and Eps8 was demonstrated by the in vivo co-immunoprecipitation and colocalization assays and the in vitro GST pull-down assays. Furthermore, we mapped the interaction domains to the region between amino acids 260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition, protein stability assays and immunofluorescence analysis showed ITSN2 overexpression induced the degradation of Eps8 proteins, which was markedly alleviated with the lysosome inhibitor NH4Cl treatment. Taken together, our results suggested ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins. [BMB reports 2012; 45(3): 183-188]