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Said Zul Amraini,Lina Putri Ariyani,Heri Hermansyah,Siswa Setyahadi,Siti Fauziyah Rahman,박돈희,Misri Gozan 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.3
Oil Palm Empty Fruit Bunch (OPEFB) is an abundant biomass resource in Indonesia, which contains 41.3 ~ 46.5% (w/w) of cellulose. This research examined the production of cellulase by the E. coli EgRK2 recombinant strain using an OPEFB substrate. The production of the enzyme was initially examined to identify optimum growth conditions, by observing the growth and activity of E. coli EgRK2 compared to its wild type. Our results showed that the optimum production time, pH and temperature of the recombinant growth and cellulase activity were achieved at 24 h, and at 7 and 40°C, respectively. Using these optimum conditions, the enzyme was produced, and experiments were carried out to examine the enzyme characteristics, produced from both strains, on hydrolysis of cellulose from OPEFB. Our results showed that the activity of the enzyme produced by the recombinant almost doubled compared to that of the wild type, although the optimum pH for both strains was pH 6. Higher activity was achieved by the recombinant compared to the wild type strain, and values were 1.905 and 1.366 U/mL, respectively. The optimum temperature for hydrolysis by cellulase occurred at 50°C for Bacillus sp. RK2, and 60°C for Bacillus sp. EgRK2. The Michaelis-Menten constant (Km) and maximum velocity (Vmax) for OPEFB degradation by E. coli EgRK2 were 0.26% and 1.750 μmol/mL/sec, which were significantly better values than those of the wild type. Control experiments for the degradation test using CMC also showed a better Vmax value for E. coli EgRK2 compared to the wild type, which is 2.543 and 1.605 μmol/mL/sec, respectively.
Anondho Wijanarko,Dianursanti,Antonius Yudi Sendjaya,Heri Hermansyah,Arief Budi Witarto,Misri Gozan,Bondan Tiara Sofyan,Kazuhiro Asami,Kazuhisa Ohtaguchi,Roekmijati Widaningroem Soemantojo,송승구 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.4
Microalgae perform oxygenic photosynthesis and are capable of taking up a large amount of CO₂, using an inducible CO₂ concentrating mechanism (CCM), and fixing CO₂ into higher compounds. These characteristics make the microalgae potentially useful for removal and utilization of CO₂ emitted from industrial plants and, generally, the usage of photosynthetic microorganisms has increased and significantly improved as a solution for CO₂ emissions. In this light and based on previous research using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement was sought for CO₂fixation and biomass production by Chlorella vulgaris Buitenzorg by increasing the photon flux density concurrent with increases in culture biomass during the cellular growth phase and was compared to cultures of Chlorella grown at optimal constant illumination, with all cultures grown using Bennick basal medium, 29℃, and a flow of 1.0 atm. 10% CO₂ enriched air delivered to three in serial photobioreactors of 0.200 dm³capacity each. The results showed that increasing illumination during culture increased biomass production of Chlorella by ~60% as well as increased CO₂ fixation ability by ~7.0%. It was also demonstrated that the non-competitive inhibition of [HCO₃-] as a carbon source significantly affected the cultivation in both the increasing and constant photon flux density regimes.
Wijanarko Anondho,Dianursanti Dianursanti,Gozan Misri,Andika Sang Made Krisna,Widiastuti Paramita,Hermansyah Heri,Witarto Arief Budi,Asami Kazuhiro,Soemantojo Roekmijati Widaningroem,Ohtaguchi Kazuhis The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
Alteration of illumination with optimum carbon dioxide fixation-based curve in this research successfully enhanced the $CO_{2}-fixation\;(q_CO_{2}$ capability of Chlorella vulgaris Buitenzorg cultivated in a bubble column photo bioreactor. The level of $CO_{2}$ fixation was up to 1.91 times that observed from cultivation with intensification of illumination on an optimum growth-based curve. During 144 h of cultivation, alteration of light intensity on an optimum $CO_{2}-fixation-based$ curve produced a $q_CO_{2}$ of $12.8\;h^{-1}$. Meanwhile, alteration of light intensity with a growth-based curve only produced a $q_CO_{2}$ of $6.68\;h^{-1}$. Increases in light intensity based on a curve of optimum $CO_{2}-fixation$ produced a final cell concentration of about 5.78 g/L. Both cultivation methods were carried out under ambient pressure at a temperature of $29^{\circ}C$ with a superficial gas velocity of $2.4\;m/h(U_{G}$. Cells were grown on Beneck medium in a 1.0 L Bubble Column Photo bioreactor illuminated by a Phillips Halogen Lamp (20 W/12 V/50 Hz). The inlet gas had a carbon dioxide content of 10%.
Song Seung Koo,Anondho Wijanarko,Dianursanti,Misri Gozan,Sang Made Krisna Andika,Paramita Widiastuti,Heri Hermansyah,Arief Budi Witarto,Kazuhiro Asami,Roekmijati Widaningroem Soemantojo,Kazuhisa Ohtag 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
Alteration of illumination with optimum carbon dioxide fixation-based curve in this research successfully enhanced the CO2-fixation (qCO2) capability of Chlorella vulgaris Buitenzorg cultivated in a bubble column photo bioreactor. The level of CO2 fixation was up to 1.91 times that observed from cultivation with intensification of illumination on an optimum growth-based curve. During 144 h of cultivation, alteration of light intensity on an optimum CO2-fixation-based curve produced a qCO2 of 12.8 h1. Meanwhile, alteration of light intensity with a growth-based curve only produced a qCO2 of 6.68 h1. Increases in light intensity based on a curve of optimum CO2-fixation produced a final cell concentration of about 5.78 g/L. Both cultivation methods were carried out under ambient pressure at a temperature of 29oC with a superficial gas velocity of 2.4 m/h (UG). Cells were grown on Beneck medium in a 1.0 L Bubble Column Photo bioreactor illuminated by a Phillips Halogen Lamp (20 W/12 V/50 Hz). The inlet gas had a carbon dioxide content of 10%.