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        Study on accelerated life tests for main shaft bearings in wind turbines

        Sheng Jin,Hanjie Dong,Jie Chen,Xinghui Xie,Mingjun Guo 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.3

        This paper focused on a 2.1 MW wind turbine main shaft bearing as the research object and analyzed its reliability under actual working conditions for three years. An accelerated life test for the main shaft bearing in a wind turbine with an amplified load was carried out depending on the reference value of the radial dynamic load rating. The test was conducted for 140 days. The bearing did not show any noticeable damage at the end of the test, which shows that the bearing could be reliable for three years. To prove the correctness of the ALT, a finite element model of the main shaft bearing was created in ABAQUS to obtain the contact stress in both the actual working conditions and the accelerated test conditions. The calculation results were transferred to FE-SAFE to calculate the fatigue life. Finally, a comparison between the theoretical and simulation acceleration factors validated the rationality of the experimental design.

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        D-Lactic Acid Production by Sporolactobacillus inulinus Y2-8 Immobilized in Fibrous Bed Bioreactor Using Corn Flour Hydrolyzate

        ( Ting Zhao ),( Dong Liu ),( Hengfei Ren ),( Xinchi Shi ),( Nan Zhao ),( Yong Chen ),( Hanjie Ying ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.12

        In this study, a fibrous bed bioreactor (FBB) was used for D-lactic acid (D-LA) production by Sporolactobacillus inulinus Y2-8. Corn flour hydrolyzed with α-amylase and saccharifying enzyme was used as a cost-efficient and nutrient-rich substrate for D-LA production. A maximal starch conversion rate of 93.78% was obtained. The optimum pH for D-LA production was determined to be 6.5. Ammonia water was determined to be an ideal neutralizing agent, which improved the D-LA production and purification processes. Batch fermentation and fedbatch fermentation, with both free cells and immobilized cells, were compared to highlight the advantages of FBB fermentation. In batch mode, the D-LA production rate of FBB fermentation was 1.62 g/l/h, which was 37.29% higher than that of free-cell fermentation, and the D-LA optical purities of the two fermentation methods were above 99.00%. In fed-batch mode, the maximum D-LA concentration attained by FBB fermentation was 218.8 g/l, which was 37.67% higher than that of free-cell fermentation. Repeated-batch fermentation was performed to determine the long-term performance of the FBB system, and the data indicated that the average D-LA production rate was 1.62 g/l/h and the average yield was 0.98 g/g. Thus, hydrolyzed corn flour fermented by S. inulinus Y2-8 in a FBB may be used for improving D-LA fermentation by using ammonia water as the neutralizing agent.

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        Feasibility Study on Long-Term Continuous Ethanol Production from Cassava Supernatant by Immobilized Yeast Cells in Packed Bed Reactor

        Liu Qingguo,Zhao Nan,Zou Yanan,Ying Hanjie,Dong Liu,Chen Yong 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

        In this study, yeast cell immobilization was carried out in a packed bed reactor (PBR) to investigate the effects of the volumetric capacity of carriers as well as the different fermentation modes on fuel ethanol production. An optimal volumetric capacity of 10 g/l was found to obtain a high cell concentration. The productivity of immobilized cell fermentation was 16% higher than that of suspended-cell fermentation in batch and it reached a higher value of 4.28 g/l/h in repeated batches. Additionally, using this method, the ethanol yield (95.88%) was found to be higher than that of other tested methods due to low concentrations of residual sugars and free cells. Continuous ethanol production using four bioreactors showed a higher productivity (9.57 g/l/h) and yield (96.96%) with an ethanol concentration of 104.65 g/l obtained from 219.42 g/l of initial total sugar at a dilution rate of 0.092 h-1. Furthermore, we reversed the substrate-feed flow directions in the in-series bioreactors to keep the cells at their highest activity and to extend the length of continuous fermentation. Our study demonstrates an effective method of ethanol production with a new immobilized approach, and that by switching the flow directions, traditional continuous fermentation can be greatly improved, which could have practical and broad implications in industrial applications.

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