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Hanbin Lin,Allan Wee Ren Ng,Chen Wai Wong 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.suppl1
Purification and characterization of polyphenol oxidase (PPO) from Chinese parsley (Coriandrum sativum) were achieved. Crude PPO exhibited an enzyme activity of 1,952.24 EU/mL. PPO was partially purified up to 6.52x with a 10.89% yield using gel filtration chromatography. Maximal PPO activity was found at 35oC, pH 8.0 for 4-methylcatechol and at 40oC, pH 7.0 for catechol. PPO showed a higher affinity towards 4-methylcatechol, but a higher thermal stability when reacting with catechol. LCysteine was a better inhibitor than citric acid for reducing PPO activity at concentrations of 1 and 3mM in the presence of either substrate. Two 46 kDa isoenzymes were identified using SDS-PAGE. Isolation and characterization of Chinese parsley serves as a guideline for prediction of enzyme behavior leading to effective prevention of enzymatic browning during processing and storage, including inhibition and inactivation of PPO.
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Experimental and numerical studies of miniature bar-typed structural fuses with Teflon pads
Sen Yang,Yan Liu,Yu Lin,Dongzhi Guan,Hanbin Ge,Zhengxing Guo,Wenguang Liu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.4
Many studies have proved that structural fuses could improve the seismic performance of structures efficiently. A structural fuse named the miniature bar-typed structural fuse (MBSF) has been proposed and investigated by the authors, which consists of a central core bar, a confining tube. To further improve the mechanic performances of the MBSFs under compressive loadings, Teflon pads are introduced to adjust the contact and friction status between the core bar and the confining tube. Three groups of specimens were discussed including the specimen with a single cutting line (SC), the specimen with double cutting lines (DC), and the specimen with triple cutting lines (TC). The results show that the hysteretic performances of the fuses are improved with the help of Teflon pads. The compression strength adjustment factor declines when Teflon pads are appended. Numerical and theoretical analyses are also conducted which expounded the effect of the Teflon pads. Different plastic buckling deformation principles of the core bars are compared by the theoretical analysis. It is shown that Shanley’s theory fits the numerical results well, which is recommended for the theoretical calculation of the proposed MBSFs.
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