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Study on the surface pressure distribution of cubes in cross-wind arrays
Lim, H.-C.,Tsukamoto, K.,Ohba, M.,Mizutani, K. Elsevier Scientific Pub. Co 2014 Journal of wind engineering and industrial aerodyn Vol.133 No.-
In this study, effects of the gap on equal-spaced cubical bodies (150mmx150mmx150mm) placed in a turbulent boundary layer were investigated inside an atmospheric boundary layer wind tunnel. This study includes various measurements of surface-pressure distribution around a building in close proximity to surrounding obstacles. In addition, we examined the surface-pressure variation with cube distances (G) at 75mm (0.5h), 150mm (1h), 225mm (1.5h) and ~ (i.e., a single cube). The experiments conducted included some boundary layer wind tunnel tests with Hot Wire Anemometry (HWA) and mean and fluctuating surface-pressure measurements around a set of cubes aligned in parallel. The tunnel tests were carried out at two different Reynolds numbers (Re=4.6x10<SUP>4</SUP> and 6.7x10<SUP>4</SUP>), based on wind velocity U<SUB>h</SUB> (4.5m/s and 7.3m/s) at a cube height h. On analyzing the results, we discovered that the gap effect of surrounding models has a significant influence on the pressure variation around the central model. The overall surface-pressure coefficient around the central structure was generally found to increase as the gap (G) between the structures was increased.
Kim, K.-H.,Wasano, N.,Ohba, M. 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1
Four Lepidoptera-specific reference strains of Bacillus thuringiensis, belonging to serovars sumiyoshiensis(H3a:3d), fukuokaensis(H3a:3d:3e), darmstadiensis(H10a:10b) and japonensis(H23), which produce spherical parasporal inclusions, were examined for comparative characterization of δ-endotoxins. SDS-PAGE profiles of the alkali-solubilized parasporal inclusions revealed the presence of single major protein bands of 130 kDa in the four strains. Chymotrypsin and trypsin treatment of the proteins gave profiles different from those of the strains HD-1(serovar kurstaki, H3a:3b:3c) and T84Al(serovar sotto, H4a:4b). Also, minor variations were observed in proteolysis profiles among the four strains. The LC_50 values of purified parasporal inclusions for the silkworm(Bombyx mori) larvae were 7·35, 6·45, 3·08 and 2·63 ㎍ g^-1 diet, respectively, showing that their toxicity levels were 5-15 times lower than that of the strain HD-1 (0·49㎍ g^-1 diet). Analysis by immunodiffusion and immunoblotting with polyclonal antisera revealed that parasporal inclusion proteins of the four strains are highly related, whereas they shared few or no common anitgens with those of the strains HD-1, T84Al and Buibui(serovar japonensis).
Park, J.I.,Kim, S.G.,Chun, J.S.,Seo, Y.M.,Jeon, M.J.,Ohba, M.,Kim, H.J.,Chun, S.Y. North-Holland 2007 Molecular and cellular endocrinology Vol.270 No.1
We have previously demonstrated that luteinizing hormone (LH) induces a rapid and transient expression of NGFI-B in the ovary. In this report, we investigated the signaling pathway for LH- and forskolin-induced NGFI-B expression in cultured rat granulosa cells of preovulatory follicles. LH- or forskolin-induced NGFI-B expression was suppressed by high dose of protein kinase C (PKC) inhibitor RO 31-8220 (10μM), but not by low doses RO 31-8220 (0.1-1.0μM) or adenylate cyclase inhibitor MDL-12,300A, implicating the involvement of atypical PKCs. Kinase assay revealed that LH treatment of granulosa cells resulted in a rapid stimulation of atypical PKCζ activity. Interestingly, like LH, forskolin was also able to activate PKCζ. Treatment with the cell-permeable PKCζ-specific inhibitor pseudosubstrate peptide inhibited LH-or forskolin-induced NGFI-B expression, indicating the essential role of PKCζ. Consistent with this promise, in granulosa cells depleted of diacylglycerol sensitive PKCs by prolonged treatment with tetradecanoylphobol-13-acetate, LH or forskolin could still induce NGFI-B expression, and RO 31-8220 or the PKCζ pseudosubstrate peptide inhibited LH- or forskolin-induced NGFI-B expression. Furthermore, overexpression of dominant-negative PKCζ in primary granulosa cells using a replication-defective adenovirus vector resulted in the suppression of LH- or forskolin-induced NGFI-B expression. Our findings demonstrate that PKCζ, which is activated by LH or forskolin, contributes to the induction of NGFI-B in granulosa cells of preovulatory follicles.