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Mario Ramirez,Luis P. Iniguez,Gabriela Guerrero,Francesca Sparvoli,Georgina Hernandez 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.6
The common bean (Phaseolus vulgaris L.)– Rhizobium etli symbiosis and crop productivity are highly affected by adverse environmental conditions that cause oxidative stress. Based on the improved symbiosis of common bean inoculated with engineered R. etli expressing the Vitreoscilla hemoglobin (VHb) (Ramı ´rez et al., Mol Plant Microbe Interact 12:1008–1015, 1999), in this work we analyzed the effect of this strain in plants exposed to the herbicide paraquat (PQ) which generates oxidative stress. PQ-treated plants inoculated with the engineered (VHb) R. etli strain showed higher nitrogenase activity and ureide content than plants inoculated with the wild type strain. We performed microarray transcriptomic analysis to identify PQ-responsive genes in nodules elicited by engineered vs wild type strains. An evident reprogramming of the transcriptional profile was observed in PQ-treated nodules, and the global changes in gene expression were different between nodules elicited with each strain. The most relevant difference was the increased number of up-regulated PQ-responsive genes in wild type strain nodules as compared to VHb-expressing nodules. The majority of these genes were classified into biological processes/functional categories related to defense, response to abiotic stress or signaling, as revealed by Gene Ontology and MapMan analysis. Taken together our analysis suggests that the expression of VHb in R. etli bacteroids contributes to buffering the damage caused by increased reactive oxygen species, and this is reflected in nodule cells that showed decreased sensitivity to oxidative stress and response of stress-related genes. Biotechnological applications of VHbexpressing rhizobia inoculants could be further explored.
Regeneration of fat cells from myofibroblasts during wound healing
Plikus, Maksim V.,Guerrero-Juarez, Christian F.,Ito, Mayumi,Li, Yun Rose,Dedhia, Priya H.,Zheng, Ying,Shao, Mengle,Gay, Denise L.,Ramos, Raul,Hsi, Tsai-Ching,Oh, Ji Won,Wang, Xiaojie,Ramirez, Amanda,K American Association for the Advancement of Scienc 2017 Science Vol.355 No.6326
<P>Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed in mammals. We discovered in the mouse that during wound healing, adipocytes regenerate from myofibroblasts, a cell type thought to be differentiated and nonadipogenic. Myofibroblast reprogramming required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling and then activation of adipocyte transcription factors expressed during development. Overexpression of the BMP antagonist Noggin in hair follicles or deletion of the BMP receptor in myofibroblasts prevented adipocyte formation. Adipocytes formed from human keloid fibroblasts either when treated with BMP or when placed with human hair follicles in vitro. Thus, we identify the myofibroblast as a plastic cell type that may be manipulated to treat scars in humans.</P>
Robust Nonlinear Control of AC Brushless Motor for Electric Vehicles Application
Langarica-Cordoba, Diego,Guerrero-Ramirez, Gerardo V.,Claudio-Sanchez, Abraham,Duran-Fonseca, Miguel A.,Adam-Medina, Manuel,Astorga-Zaragoza, Carlos-Manuel The Korean Institute of Power Electronics 2011 JOURNAL OF POWER ELECTRONICS Vol.11 No.4
This article proposes a robust nonlinear control based on Lyapunov's redesign, whose purpose is to deal with parametric uncertainty in the resistance of the motor windings. The robust controller design is based on the passivity properties of the motor, as well as energy shaping and damping injection. The application of this control technique is focused on electric vehicles mainly formed by a battery bank, a power inverter, an AC brushless motor and the mechanical transmission. The sine PWM technique is used to trigger the switching devices of inverter. The results were obtained from simulation, where is shown that robust control makes a proper tracking of electromagnetic torque.
Robust Nonlinear Control of AC Brushless Motor for Electric Vehicles Application
Diego Langarica-Cordoba,Gerardo V. Guerrero-Ramirez,Abraham Claudio-Sanchez,Miguel A. Duran-Fonseca,Manuel Adam-Medina,Carlos-Manuel Astorga-Zaragoza 전력전자학회 2011 JOURNAL OF POWER ELECTRONICS Vol.11 No.4
This article proposes a robust nonlinear control based on Lyapunov’s redesign, whose purpose is to deal with parametric uncertainty in the resistance of the motor windings. The robust controller design is based on the passivity properties of the motor, as well as energy shaping and damping injection. The application of this control technique is focused on electric vehicles mainly formed by a battery bank, a power inverter, an AC brushless motor and the mechanical transmission. The sine PWM technique is used to trigger the switching devices of inverter. The results were obtained from simulation, where is shown that robust control makes a proper tracking of electromagnetic torque.