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RISS 인기검색어
- 검색키워드
- 저자 : Giuseppe Piccione (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
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Training-induced changes in clotting parameters of athletic horses
Giuseppe Piccione,Marilena Bazzano,Claudia Giannetto,Simona Marafioti,Francesco Fazio 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.1
The purpose of this study was to investigate the effects oftraining on prothrombin time, activated partial thromboplastintime, and fibrinogen (Fb) concentrations in horses to assesspotential adaptive response to training. Fifteen clinicallyhealthy horses were enrolled in the present study and equallydivided into three groups. Group A completed an intensetraining program, group B participated in a light trainingprogram, and group C included sedentary horses. After 5 weeks,group B was subjected to the same training program completedby group A and renamed group B1. Blood samples werecollected by jugular venipuncture from each animal at rest andanalyzed within 2 h after sampling. A two-way ANOVA forrepeated measures showed a significant effect of training (p <0.05) on Fb concentrations in group B1 alone during the firstweek after changing the training program. Our findingsdemonstrated that Fb is a parameter susceptible to training. Fbplasma levels increase with a more intense training program. However, Fb plasma levels decreased after the first week andreturned to basel levels, suggesting that the horses had adaptedto the new training program.
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Giuseppe Piccione,Stefania Casella,Claudia Giannetto,Elisabetta Giudice 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.2
The present study was to assess the effect of storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen concentration in blood samples of healthy dogs. Thirty-five dogs of various breeds were included in the study. Citrated blood samples were obtained and plasma was divided into four aliquots to assess selected clotting parameters by means of a coagulometer. The first aliquot was analysed within 1 h after collection, while the remaining 3 were stored at 8oC for 4, 8 and 24 h, respectively. One-way repeated measures analysis of variance documented a significant decreasing effect on PT at 24 h compared to 8 h and on fibrinogen concentration after 8 and 24 h compared to sampling time and at 4 and 24 h compared to 8 h post sampling. In conclusion, the results of this study indicate that only fibrinogen appears prone to significant decrease. In fact, aPTT is not substantially affected by refrigeration for at least 24 h post sampling and PT showed a statistical difference that does not necessary indicate biological significance as the results obtained were within reference intervals for the dog.
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Echocardiography and electrocardiography as means to evaluate potential performance in horses
Giuseppe Piccione,Carlos Lightowler,Elisabetta Giudice,Gerardo Romei del Olmo,Maria Laura Cattaneo 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.3
Prediction of potential performance is one of the goals of exercise physiology investigations. When Selecting a horse for competition, one of the main objectives is to choose the one that predictably will reveal a competitive aptitude above the average. The horses used in this study underwent a two-dimensional echocardiography study and a conventional 3 leads electrocardiogram. The results show that heart score is not an appropriate index to evaluate the heart size in the horse. On the other hand, there are currently more suitable and accurate procedures such as echocardiography that allow performing a clear anatomical evaluation and accurate measurement in order to calculate LVMM and to predict performance.
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Peripheral serotoninergic response to physical exercise in athletic horses
Daniela Alberghina,Claudia Giannetto,Giuseppe Piccione 대한수의학회 2010 JOURNAL OF VETERINARY SCIENCE Vol.11 No.4
The purpose of this study was to evaluate the influence of exercise on plasma tryptophan (TRP) and free serotonin (f5-HT), whole blood-5-HT (WB-5-HT) and f5-HT/WB-5-HT ratio in Italian Saddle horses. Six clinically healthy Italian Saddle horses were subjected to a 450 meters obstacles course. Blood samples were collected from each horse by jugular venipuncture using vacutainer tubes with K3-EDTA at rest, immediately after exercise, and after 30 min. TRP, f5-HT and WB-5-HT were analyzed by HPLC. Immediately after exercise, statistically significant increases of f5-HT (p<0.001) and WB-5-HT (p<0.001) were observed. After 30 min, f5-HT and WB-5-HT decreased compared to immediately after exercise, but were still significantly higher than rest values (p<0.01 and p<0.05, respectively). A significant linear regression between f5-HT and WB-5-HT was observed during experimental conditions. f5-HT and WB-5-HT modifications after exercise suggest an important role of peripheral serotoninergic markers in response to physical activity. The possible source of extra serotonin detected after show jumping should be clarified by further investigation.
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Effects of hydrocortisone and aminophylline on the aggregation of equine platelets in vitro
Stefania Casella,Elisabetta Giudice,Claudia Giannetto,Simona Marafioti,Giuseppe Piccione 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.3
The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided into three different aliquots. In the first aliquot, platelet aggregation was measured after platelet activation with 1 μM and 0.5 μM ADP (Group A). In the other two aliquots, the effect of a 10 min preincubation with hydrocortisone (Group B) or aminophylline (Group C) on ADP-induced aggregation at final ADP concentrations of 1 μM and 0.5 μM was observed. Platelet aggregation, recorded by an aggregometer, was evaluated by measuring the maximum degree of platelet aggregation and the initial velocities of platelet aggregation were obtained. Our results demonstrated the inhibitory effect of hydrocortisone and the induction effect of aminophylline on equine platelet responses in vitro.
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