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- 저자 : Ghanavati Farangis (검색결과 2 건)
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Mirzahosein-Tabrizi Maryam,Ghanavati Farangis,Azizinezhad Reza,Etminan Alireza 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.3
Genetic diversity is a key factor for breeding programs and provides opportunities for developing superior varieties with preferred traits. Trigonella is a large genus of plants that is important in culinary terms and for the food industry. The present research aimed to study genetic diversity and population structure in a set of 90 accessions from eight diferent species of Trigonella sp. using 20 URP and SCoT primers. Also, fve amino acids and Trigonelline content were determined in the plants. The average marker index was 3.43 and 3.73 for SCoT and URP primers, respectively. NJ clustering was based on combined data and classifed all 90 accessions clearly, based on their species. AMOVA revealed that the main part (68%) of total variation occurring within species and T. monantha was the most diverse population among all species. According to the phytochemical, there were signifcant diferences between all species in terms of trigonelline content and the amounts of amino acids. High levels of genetic diversity were identifed among and within the eight species which can be considered for breeding purposes. Furthermore, URP and SCoT were found useful markers for the analysis of genetic diversity and population structures in Trigonella sp. Meanwhile, Trigonella foenum-graecum showed the highest Trigonelline content. As a matter of the health benefts provided by this alkaloid, T. foenum-graecum can be used not only for medicinal purposes but also as a useful vegetable and food ingredient.
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Ghobadi-Namin Leila,Etminan Alireza,Ghanavati Farangis,Azizinezhad Reza,Abdollahi Parisa 한국작물학회 2024 Journal of crop science and biotechnology Vol.27 No.1
The importance of plant genetic resources has received increasing attention in crop improvement and plant variation in breeding procedures. Here, plant variation and the structures of plant populations were examined among 93 flax accessions using SCoT and URP molecular markers. Polymorphic-fragments by URP and SCoT primers were, on average, 9.3 and 10.9, respectively, and the mean of polymorphism information content for primers revealed a good efficiency of both marker techniques (0.34 and 0.35 for URP and SCoT, respectively). The neighbor-joining (NJ)-based clustering, via combined data, classified all investigated accessions into four main groups that were further confirmed by principal coordinate analysis (PCoA). Moreover, molecular diversity (88%) was mostly observed inside the populations. STRUCTURE-analysis confirmed the cluster analysis and all samples were separated into four subpopulations (∆K = 4). The results implied that the sub-populations consisted of diverse accessions, suggesting that this gene pool has the potential for flax breeding programs. Furthermore, our findings indicated that URP and SCoT markers are reliable techniques in assessing plant variation, especially in elucidating the structures of flax populations. However, gene-targeting markers like SCoT are preferable because of their roots in functional genome-based regions.
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