http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ganesan Arun,Muthukumarasamy Eyini,Paramasamy Gunasekaran 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
Silver nanoparticles, which are small metalliccolloidal particles, ranging 1 ~ 100 nm in size, have severalnano biotechnological applications in medicine, manufacturingand engineering industries. Fungus-mediated synthesis ofsilver nanoparticles is an ecofriendly, green process. Further,extracellular enzymes and proteins elaborated by fungi areinvolved in the synthesis of the silver nanoparticle, whichmakes the downstream processing relatively simpler. In thepresent investigation, Schizophyllum commune, a mushroomfungus, was tested for its ability to synthesize extracellularas well as intracellular silver nanoparticles. When thefungus was challenged with 1 mM silver nitrate, a changein colour of the broth and the mycelium was observed,indicative of extracellular and intracellular synthesis ofsilver nanoparticles. The presence of silver nanoparticleswas confirmed by studying its Surface Plasmon Resonanceabsorption band in the visible wavelength. FTIR spectrumanalysis of the silver nanoparticles indicated the presenceof biomolecules in association with the reduction of silverions. Scanning Electron Microscopic analysis of the silvernanoparticles revealed the nanorange dimensions of boththe extracellular and the intracellular silver nanoparticles. Analysis of biological activities of the silver nanoparticlesdisclosed their significant antibacterial activity againstEscherichia coli, Bacillus subtilis, Klebsiella pneumoniaeand Pseudomonas fluorescens. Additionally, the silvernanoparticles inhibited the growth of the dermatophyticfungal pathogens viz. Trichophyton simii, Trichophytonmentagrophytes and Trichophyton rubrum significantly. Anticancer activity of silver nanoparticles, assayed throughMTT cytotoxicity assay, uncovered that 27.2 and 64%mortality could be obtained in Human Epidermoid LarynxCarcinoma (HEP -2) cell lines at concentrations between10 and 100 μg/mL, respectively. The results obtained indicatethat Schizophyllum commune is capable of synthesizingsilver nanoparticles in shaken broth cultures (120 rpm) at25 ± 2°C and pH 7.
Rao, Arunagiri Kuha Deva Magendhra,Vinothkumar, Vilvanathan,Revathidevi, Sundaramoorthy,Arunkumar, Ganesan,Manikandan, Mayakannan,Arun, Kanagaraj,Rajkumar, Kottayasamy Seenivasagam,Ramani, Rajendran,R Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21