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RISS 인기검색어
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- 저자 : Franklin H. Mancia (검색결과 3 건)
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Franklin H. Mancia,Jung Sun Kim,Raisa Aone M. Cabahug,Yoon-Jung Hwang 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.2
Sisymbrium irio (2n = 2x = 14) is a wild plant with traits that can off er economic and ecological benefits, yet it has received scant attention compared to its closely related species. There is no substantial genomic information generated from this species, thus highlighting the need for molecular cytogenetic analyses. The information provided from karyotypic investigation is essential for developing cytogenetic maps. In the present study, asymmetry/symmetry indexes classified S. irio as having a moderately symmetric karyotype. Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) techniques were implemented as important cytogenetic tools for the direct detection of chromosomal targets and the study of the evolutionary relationship of crops. Repetitive DNA sequences such as ribosomal DNA (rDNA), C 0 t DNA, telomeric repeats, satellite repeats, and genomic DNA sequences were used as markers for the cytomolecular characterization of S. irio . The linked arrangement of 5 and 18 S rDNA units was found to be located at the terminal regions of the two chromosomes. Arabidopsis -type telomere repeats were detected in the terminal regions. Interestingly, labeled self-gDNA and C 0 t DNA hybridized in the pericentromeric and rDNA regions signifying the preferential distribution of the major repeats. Comparative GISH assays revealed the degree of genomic relationships among S. irio , Raphanus sativus , and Brassica diploids. The cytogenetic maps generated in this study are essential for understanding the genomic organization of S. irio and could be utilized for future validation of its genome assembly.
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배추에서 Ribosomal DNAs 분포의 비교분자세포유전학적 분석
Franklin H. Mancia,김정선(Jung Sun Kim),황윤정(Yoon-Jung Hwang) 한국육종학회 2021 한국육종학회지 Vol.53 No.3
Three Brassica species, namely, Brassica rapa, B. nigra, and B. oleracea are considered economically important as they are grownfor human consumption and biogas production. Like other crops facing agricultural constraints, selective crossing or hybridization in cruciferousvegetables has helped farmers to improve them. This study conducted a comparative evaluation across and within the species through cytogeneticanalysis to provide fresh insights into their chromosome structures and evolutionary relationships. A new karyomorphological parameter confirmedsymmetric karyotypes in all the accessions, thereby allowing for the depiction of the ancestral chromosome karyotype. Several lines of B. rapa, B. nigra, and B. oleracea were subjected to physical mapping using a fluorescence in situ hybridization technique to elucidate thechromosomal distribution of the two types of rDNAs. The signal number and distribution of 18S rDNA across the metaphase chromosomesof B. rapa accessions did not vary as compared to 5S rDNA, which was also observed in several lines of B. nigra. In contrast, the numberand distribution of 5S rDNA loci across the chromosomes in several lines of B. oleracea were found to be more conserved than those correspondingto the 18S rDNA. Overall, this study revealed the evolutionary dynamics of rDNA, which may play an important role in shaping the chromosomekaryotypes of Brassica species.
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