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- 저자 : Fengyi Hu (검색결과 2 건)
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Analysis of ESTs from a Normalized cDNA Library of the Rhizome Tip of Oryza longistaminata
Ting Zhang,Lijuan Li,Fengyi Hu,Xiuqin Zhao,Binying Fu,Daichang Yang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1
Oryza longistaminata, a perennial wild rice species with an AA genome, is characterized by the presence of rhizomatous stems. The rhizomatous trait in rice was previously shown to be quantitatively controlled by many genes, but the molecular mechanism related to rhizome development is still unknown. In the present study, expressed sequence tags (ESTs) generated from rhizome tips of O. longistaminata were collected and analyzed. A total of 10,283 complimentary deoxyribunucleic acid clones were randomly sequenced, which generated 10,136 raw sequences,and finally, 4,419 unisequences with diverse functional categories were generated. These unisequences were mapped onto the Oryza sativa genome, which revealed that 4,285(96.97%) and 4,151 (93.94%) of the unisequences were alignable to the japonica and indica genomic sequences,respectively, with >80% sequence identity. Additionally, 41unisequences showed four typical types of alternative splicing patterns. More than 600 simple sequence repeats were identified in these unisequences. A subset of unisequences were physically colocalized onto rhizome-related quantitative trait locus intervals in rice and sorghum, and one gene,OLRR1, was further confirmed to be enriched in the rhizome tip and young leaf by real-time polymerase chain reaction and in situ hybridization. Unisequences reported in this study provide valuable data for molecular dissection of the rhizomatous growth habit in O. longistaminata.
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Comparative Metabolite Profiling and Hormone Analysis of Perennial and Annual Rice
Xiuqin Zhao,Ting Zhang,Liyu Huang,Huimin Wu,Fengyi Hu,Fan Zhang,Linghua Zhu,Binying Fu 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1
Perenniality is one of the important topics in rice breeding which is generally accompanied by complex physiobiochemical processes. To understand the metabolic characteristics of perennial rice, in the present study, gas chromatography–mass spectrometry and enzyme-linked immunosorbent assays were used to profile the distribution patterns of 33 primary metabolites and hormones [indole-3-acetic acid (IAA) and zeatin riboside (ZR)] of annual (RD23) and perennial (Oryza longistaminata and the line AA with RD23 genetic background) rice genotypes. Results showed that both metabolites and hormones have distinct genotype and organ distribution patterns, and considerable variations were observed between the metabolites in stem bases of perennial and annual rice. Most of the metabolites,including sugars, organic acids, and amino acids, significantly accumulated in the stem bases of perennial rice by decreasing the level in roots and leaves. Fifteen metabolites consistently accumulated significantly in the stem bases of both perennial genotypes. Additionally, the organ-level IAA content and IAA/ZR ratio in the two perennials were considerably higher than those in RD23. The present study indicated that the significant accumulation of the metabolites at stem base and the higher IAA/ZR ratio are involved
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