Can Wideband Tympanometry Be Used as a Screening Test for Superior Semicircular Canal Dehiscence?

Emine Demir,Nazife Nur Afacan,Metin Celiker,Fatma Beyazal Celiker,Mehmet Fatih İnecikli,Suat Terzi,Engin Dursun 대한이비인후과학회 2019 Clinical and Experimental Otorhinolaryngology Vol.12 No.3

Objectives. We explored whether wideband tympanometry (WBT) could be used as a screening test for superior semicircular canal dehiscence (SSCD), and obtained new WBT data (given that the test is not yet in common clinical use) on patients with SSCD. Methods. We compared the WBT data of patients clinically and radiologically diagnosed with SSCD in our hospital between 2013 and 2018 to those of healthy volunteers. We compared the resonance frequency (RF), maximum absorbance frequency (MAF), and maximum absorbance ratio (MAR). The t-test was used for statistical analysis with the significance level set to P<0.05. In addition, we used receiver operating characteristic analysis to derive cutoff values for SSCD diagnosis in terms of sensitivity and specificity. Results. Seventeen patients (four with bilateral and 13 with unilateral disease; 17 ears) diagnosed with SSCD and 27 healthy volunteers (47 ears) were included. The mean RFs of the SSCD patients and healthy subjects were 548.7 Hz (range, 243 to 853 Hz) and 935.1 Hz (range, 239 to 1,875 Hz), respectively (P<0.001). The mean MARs of the SSCD patients and healthy subjects were 89.4% (range, 62% to 100%) and 82.4% (range, 63% to 99%), respectively (P=0.005). The mean MAFs of the SSCD patients and healthy subjects were 1,706.3 Hz (range, 613 to 3,816 Hz) and 2,668 Hz (range, 876 to 4,387 Hz), respectively (P<0.001). In terms of SSCD diagnosis, a MAR above 86% afforded 81% sensitivity and 77% specificity; an RF below 728 Hz, 86% sensitivity and 81% specificity; and an MAF below 1,835 Hz, 79% sensitivity and 67% specificity. Conclusion. WBT may be a useful clinical screening test for SSCD. The RF and MAF were lower, and the MAR higher, in SSCD patients than in normal controls.

Effect of model calibration on seismic behaviour of a historical mosque

Ali Demir,Halil Nohutcu,Emre Ercan,Emin Hokelekli,Gokhan Altintas 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.60 No.5

The objective of the study is to investigate the effects of model calibration on seismic behaviour of a historical mosque which is one of the most significant Ottomon structures. Seismic analyses of calibrated and noncalibrated numeric models were carried out by using acceleration records of Kocaeli earthquake in 1999. In numerical analysis, existing crack zones on real structure was investigated in detail. As a result of analyses, maximum stresses and displacements of calibrated and noncalibrated numerical models were compared each other. Consequently, seismic behaviour and damage state of historical masonry Hafsa Sultan mosque was determined as more realistic in the event of a severe earthquake.

A Novel cry2Ab Gene from the Indigenous Isolate Bacillus thuringiensis subsp. kurstaki

( Sevim Ali ),( Emine Eryuzlu1 ),( Zihni Demirba ),( Smail Demir ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

A novel cry2Ab gene was cloned and sequenced from the indigenous isolate of Bacillus thuringiensis subsp. kurstaki. This gene was designated as cry2Ab25 and its sequence revealed an open reading frame of 1,902 bp encoding a 633 aa protein with calculated molecular mass of 70 kDa and pI value of 8.98. The amino acid sequence of the Cry2Ab25 protein was compared with previously known Cry2Ab toxins, and the phylogenetic relationships among them were determined. The deduced amino acid sequence of the Cry2Ab25 protein showed 99% homology to the known Cry2Ab proteins, except for Cry2Ab10 and Cry2Ab12 with 97% homology, and a variation in one amino acid residue in comparison with all known Cry2Ab proteins. The cry2Ab25 gene was expressed in Escherichia coli BL21(DE3) cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the Cry2Ab25 protein is about 70 kDa. The toxin expressed in BL21(DE3) exhibited high toxicity against Malacosoma neustria and Rhagoletis cerasi with 73% and 75% mortality after 5 days of treatment, respectively.

Pre-coagulated landfill leachate treatment by Electro-oxidation using MMO/Ti, Pt/Ti, and graphite anodes

Senem Yazici Guvenc,Yesim Daniser,Emine Can-Guven,Gamze Varank,Ahmet Demir 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.1

In this study, sequential coagulation and electro-oxidation (EO) processes were applied to landfill leachate, which is highly contaminated and complex wastewater. Since the pollutant content of the leachate was too high, the coagulation process (poly aluminum chloride (PAC) as a coagulant) was applied as a pre-treatment to reduce the cost of the EO process. The Box-Behnken design of response surface methodology was used. The color number (CN) removal efficiency estimated by the model under optimum operating conditions was 80.1%, while it was 77.6% in the experimental studies performed under optimum conditions to verify the model conformity. Multi-metal oxide doped Ti (MMO/Ti), Pt doped Ti (Pt/Ti), and graphite were used as anode and stainless steel was used as cathode in the EO process. In the EO process in which Pt/Ti anode was used, chemical oxygen demand (COD), UV<SUB>254</SUB>, and CN removal efficiencies were 52.8, 68.1, and 85.6%, respectively under the conditions of applied current 1.25 A and the pH 5. The CN value decreased to 4.2 after the coagulation process and it was 0.6 at the effluent of the EO process.

Comparative Evaluation of the Risk of Malignancy Index Scoring Systems (1-4) Used in Differential Diagnosis of Adnexal Masses

Ozbay, Pelin Ozun,Ekinci, Tekin,Caltekin, Melike Demir,Yilmaz, Hasan Taylan,Temur, Muzaffer,Yilmaz, Ozgur,Uysal, Selda,Demirel, Emine,Kelekci, Sefa Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Background: To determine the cut-off values of the preoperative risk of malignancy index (RMI) used in differentiating benign or malignant adnexal masses and to determine their significance in differential diagnosis by comparison of different systems. Materials and Methods: 191 operated women were assessed retrospectively. RMI of 1, 2, 3 and 4; cut-off values for an effective benign or malignant differentiation together with sensitivity, specificity, negative and positive predictive values were calculated. Results: Cut-off value for RMI 1 was found to be 250; there was significant (p<0.001) compatibility at this level with sensitivity of 60%, positive predictive value (PPV) of 75%, specificity of 93%, negative predictive value (NPV) of 88% and an overall compliance rate of 85%. When RMI 2 and 3 was obtained with a cut-off value of 200, there was significant (p<0.001) compatibility at this level for RMI 2 with sensitivity of 67%, PPV of 67%, specificity of 89%, NPV of 89%, histopathologic correlation of 84% while RMI 3 had significant (p<0.001) compatibility at the same level with sensitivity of 63%, PPV of 69%, specificity of 91%, NPV of 88% and a histopathologic correlation of 84%. Significant (p<0.001) compatibility for RMI 4 with a sensitivity of 67%, PPV of 73%, specificity of 92%, NPV of 89% and a histopathologic correlation of 86% was obtained at the cut-off level 400. Conclusions: RMI have a significant predictability in differentiating benign and malignant adnexal masses, thus can effectively be used in clinical practice.

Box-Behnken design-based biodiesel wastewater treatment using sequentialacid cracking and electrochemical peroxidation process: Focus on COD, oil-greaseand volatile fatty acids removals

Senem Yazici Guvenc,Aleyna Cebi,Emine Can-Güven,Ahmet Demir,Farshid Ghanbari,Gamze Varank 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

The treatability of biodiesel wastewater by the electrochemical peroxidation process following acidificationpretreatment was investigated. Box-Behnken design, one of the experimental designs of response surface methodology,was applied to optimize the process parameters for chemical oxygen demand (COD), oil-grease, and volatile fatty acids(VFAs) removal from biodiesel wastewater. The process parameters, i.e., applied current, H2O2/COD ratio, and reactiontime, were optimized and the total cost of the process was determined. After the acidification process as a pretreatment,the COD, oil-grease, and VFAs removal efficiencies were 25.4%, 68.7%, and 50%, respectively. Optimum conditionsdetermined by the developed model for maximum oil-grease removal (98.2%) were applied: current 1.0 A, H2O2/CODratio 0.4, and reaction time 32.8 minutes. This devised Box-Behnken model predicted removal efficiencies of 45.5%,98.2%, and 49.5% for COD, oil-grease, and VFAs, respectively. The total cost of the process determined by the modeland obtained from the validation experiments was 2.03 €/m3 and 1.97 €/m3, respectively. The results of the study showedthat the applied process was efficient in pollutant removal from acidified biodiesel wastewater and the Box-Behnkendesign can be applied to explain the mechanism of the process and optimize the process parameters.

Recent developments in cancer therapy and diagnosis

Atlihan-Gundogdu Evren,Ilem-Ozdemir Derya,Ekinci Meliha,Ozgenc Emre,Demir Emine Selin,Sánchez-Dengra Bárbara,González-Alvárez Isabel 한국약제학회 2020 Journal of Pharmaceutical Investigation Vol.50 No.4

Background The cancer is serious health problem and leading cause of death in the world. Area covered There have intensively studied for diagnosis and therapy of this disease and these studies provided important insights into their mechanism of action and therapeutic/diagnostic effects. The accurence rates of cancer has dramatic increase, particularly in the developed countries. Although there are many different strategies about diagnosis and treatment for cancer, more effective new approaches are needed. Expert opinion In this review, we summarize recent developments on cancer diagnosis, radiopharmaceuticals in cancer diagnosis, nanoparticulate systems in cancer diagnosis, T cells in cancer diagnosis, cancer therapy and pharmacokinetic of anticancer drugs. We thought that while there are some current limitations such as clinical studies, ranging from diagnosis to theraphy, future improvements in cancer diagnosis and treatment will meet the most relevant issues required for the eventual approval of nano-drugs, radiopharmaceuticals, T cells in clinical practice.

The Effect of Insulin Like Growth Factor-1 on Recovery of Facial Nerve Crush Injury

Asuman Feda Bayrak,Yuksel Olgun,Ayla Ozbakan,Safiye Aktas,Can Ahmet Kulan,Gonca Kamaci,Emine Demir,Osman Yilmaz,Levent Olgun 대한이비인후과학회 2017 Clinical and Experimental Otorhinolaryngology Vol.10 No.4

Objectives. The aim of this study is to investigate the efficacy of locally applied insulin-like growth factor 1 (IGF-1) on the recovery of facial nerve functions after crush injury in a rabbit model. Methods. The rabbits were randomly assigned into three groups. Group 1 consisted of the rabbits with crush injury alone; group 2, the animals applied saline solution onto the crushed facial nerve and group 3, IGF-1 implemented to the nerve in the same manner. Facial nerve injury was first electrophysiologically studied on 10th and 42nd days of the procedure. The damage to the facial nerves was then investigated histopathologically, after sacrification of the animals. Results. In the electrophysiological study, compound muscle action potential amplitudes of the crushed nerves in the second group were decreased. In pathological specimens of the first and second groups, the orders of axons were distorted; demyelination and proliferation of Schwann cells were observed. However, in IGF-1 treated group axonal order and myelin were preserved, and Schwann cell proliferation was close to normal (P<0.05). Conclusion. Local application of IGF-1 in a slow releasing gel was found efficacious in the recovery of the facial nerve crush injury in rabbits. IGF-1 was considered worthy of being tried in clinical studies in facial nerve injury cases.