Validation of Combs prognostic scoring system in Indian recurrent glioma patients treated with re-radiation

Debnarayan Dutta(Debnarayan Dutta ),Meenu Jose(Meenu Jose ),Sruthi Kalavagunta(Sruthi Kalavagunta ),Ajay Sasidharan(Ajay Sasidharan ),Haridas Nair(Haridas Nair ),Annex H. Edappattu(Annex H. Edappattu 대한방사선종양학회 2024 Radiation Oncology Journal Vol.42 No.1

Purpose: Retrospective audit of recurrent glioma patients treated by different fractionation schedules and to validate the modified Combs prognostic score in Indian patient cohort. Materials and Methods: Between Jan 2009 and June 2022, 66 recurrent gliomas patients treated with standard adjuvant treatment—radiation (RT) ± temozolomide (chemotherapy)—and re-treated with RT (± chemotherapy) were categorized as per modified Combs prognostic criteria and outcomes were compared. Results: Sixty-six patients with recurrent gliomas who received reirradiation (re-RT) were audited—53% males; 61% Karnofsky performance status (KPS) ≥80 at time of re-RT; median age 41.5 years (range, 6 to 70 years); 67% <50 years; primary histology low-grade glioma in 33% ; grade III 27%, grade IV 40%; initial median dose of 60 Gy equivalent dose in 2 Gy fractions (EQD2); maximum safe resection at recurrence 41%; mean and median follow-up 78 ± 51 months and 66 months. Mean time interval between RT was 46.4 ± 39 months. Mean planning target volume (PTV) volume in conventional RT (Conv-RT), hypofractionated RT (Hypo-RT), and ultra-hypofractionated RT (UF-RT) was 226.1 ± 140.7 mL, 162.8 ± 123.3 mL, and 143.3 ± 145.8 mL. Mean dose for Conv-RT, Hypo-RT, and UF-RT was 50 Gy (range, 40 to 60), 31 Gy (range, 20 to 40), and 20 Gy (range, 10 to 30). Mean overall survival (OS) in Conv-RT, Hypo-RT, and UF-RT cohort was 18.8 months (range, 2.4 to 76.8); 6.6 months (range, 2 to 17.4), and 13.9 months (range, 3 to 131.9). Median OS as per Combs criteria were 16.6 months (Group a), 24.6 months (Group b), 4.6 months (Group c), and 3 months (Group d). Significant survival benefit was with good KPS score (KPS >80 vs. <80; 20.46 vs. 5.25 months; p < 0.001), patients receiving salvage chemotherapy (20.46 vs. 6.96 months; p = 0.001), and patients received re-RT biological equivalent dose (BED3) >80 Gy (16.62 vs. 5.48 months; p = 0.03). Median overall survival (OS) in our patient cohort and Combs cohort in Group a was 16.6 and 19.5 months; Group b was 24.6 and 11.3 months; Group c was 4.7 and 8.1 months, and Group d was 2 and 5.5 months, respectively. Six months survival in our patient cohort and Combs cohort in Groups a, b, c, d were 100%, 92%, 34%, 17% and 94%, 79%, 70%, 41%, respectively. Twelve months survival in our patient cohort and Combs cohort in Groups a, b, c, d were 88%, 74%, 22%, 0% and 88%, 47%, 22%, 7%, respectively. Conclusion: Modified Combs prognostic factors predicts OS and is applicable in Indian subcontinent patient population.

Evidence of Multimeric Forms of HSP70 with Phosphorylation on Serine and Tyrosine Residues - Implications for Roles of HSP70 in Detection of GI Cancers

Dutta, Anand,Girotra, Mohit,Merchant, Nipun,Nair, Padmanabhan,Dutta, Sudhir Kumar Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Heat-shock protein70 (HSP70) are intracellular protein chaperones, with emerging evidence of their association with various diseases. We have previously reported significantly elevated plasma-HSP70 (pHSP70) in pancreatic cancer. Current methods of pHSP70 isolation are ELISA-based which lack specificity due to cross-reactivity by similarities in the amino-acid sequence in regions of the protein backbone resulting in overestimated HSP70 value. Materials and Methods: This study was undertaken to develop a methodology to capture all isoforms of pHSP70, while further defining their tyrosine and serine phosphorylation status. Results: The methodology included gel electrophoresis on centrifuged supernatant obtained from plasma incubated with HSP70 antibody-coupled beads. After blocking non-specific binding sites, blots were immunostained with monoclonal-antibody specific for human-HSP70, phosphoserine and phosphotyrosine. Conclusions: Our novel immunocapture approach has distinct advantages over the commercially available methods of pHSP70 quantification by allowing isolation of molecular aggregates of HSP70 with additional ability to precisely distinguish phosphorylation state of HSP70 molecules at serine and tyrosine residues.

Hydration of ions in two-dimensional water

Dutta, S.,Lee, Yongjin,Jho, Y. S. American Physical Society 2015 Physical review. E, Statistical, nonlinear, and so Vol.92 No.4

CP asymmetries of B→φK and B→^η(′)K decays using a global fit in QCD factorization

Dutta, Bhaskar,Kim, C.S.,Oh, Sechul,Zhu, Guohuai Elsevier 2004 Physics letters: B Vol.601 No.3

<P><B>Abstract</B></P><P>We analyze the CP asymmetries of B→φK and B→<SUP>η(′)</SUP>K modes in the QCD improved factorization framework. The phenomenological parameters are determined from the global fit for the available B→PP and <I>VP</I> modes (without the subprocess b→ss¯s). The large negative sin<SUB>(2<SUB>φ1</SUB>)φ<SUB>Ks</SUB></SUB> and the large branching ratio for <SUP>B±</SUP>→<SUP>η′</SUP><SUP>K±</SUP> can be simultaneously explained in the context of R-parity conserving SUGRA models and R-parity violating SUSY models satisfying all possible experimental data. The SUSY models are also consistent with the available data on the CP asymmetries for different <SUP>B±(0)</SUP>→φ<SUP>K±(0)</SUP> and <SUP>B±(0)</SUP>→<SUP>η<SUP>(′</SUP>)</SUP><SUP>K±(0)</SUP> modes.</P>

Critical dynamics of nonconserved N-vector models with anisotropic nonequilibrium perturbations.

Dutta, Sreedhar B,Park, Su-Chan Published by the American Physical Society through 2011 Physical review. E, Statistical, nonlinear, and so Vol.83 No.1

<P>We study dynamic field theories for nonconserving N-vector models that are subject to spatial-anisotropic bias perturbations. We first investigate the conditions under which these field theories can have a single length scale. When N=2 or N수?, it turns out that there are no such field theories and, hence, the corresponding models are pushed by the bias into the Ising class. We further construct nontrivial field theories for the N=3 case with certain bias perturbations and analyze the renormalization-group flow equations. We find that the three-component systems can exhibit rich critical behavior belonging to two different universality classes.</P>

An Easily Accessible Donor−π-Acceptor-Conjugated Small Molecule from a 4,8-Dialkoxybenzo[1,2-<i>b</i>:4,5-<i>b</i>′]dithiophene Unit for Efficient Solution-Processed Organic Solar Cells

Dutta, Pranabesh,Kim, Jeongseok,Eom, Seung Hun,Lee, Woo-Hyung,Kang, In Nam,Lee, Soo-Hyoung American Chemical Society 2012 ACS APPLIED MATERIALS & INTERFACES Vol.4 No.12

<P>A new donor–acceptor-conjugated organic small molecule, <B>BDT(TBT)</B><SUB><B>2</B></SUB>, comprised of benzo[1,2-<I>b</I>:4,5-<I>b</I>′]dithiophene and 2,1,3-benzothiadiazole units was designed and synthesized. The small molecule <B>BDT(TBT)</B><SUB><B>2</B></SUB> in its thin film showed an absorption band in the range of 300–700 nm with an absorption edge at 650 nm and an optical band gap of 1.90 eV. As estimated from the cyclic voltammetry measurements, the HOMO and LUMO energy levels of <B>BDT(TBT)</B><SUB><B>2</B></SUB> were −5.44 and −3.37 eV, respectively. The spin-coated thin film of <B>BDT(TBT)</B><SUB><B>2</B></SUB> exhibited p-channel output characteristics with a hole mobility of 2.7 × 10<SUP>–6</SUP>. <B>BDT(TBT)</B><SUB><B>2</B></SUB>, when explored as an electron-donor material in solution-processed bulk-heterojunction organic solar cells in conjunction with a PC<SUB>71</SUB>BM acceptor with an active layer thickness of 50–55 nm, generated a power conversion efficiency (PCE) of 1.18%. A more impressive PCE of ∼2.9% with a short-circuit current density (<I>J</I><SUB>sc</SUB>) of 7.94 mA cm<SUP>–2</SUP> and an open-circuit voltage (<I>V</I><SUB>oc</SUB>) of 0.89 V was achieved when the active layer of the cell was annealed at higher temperature (∼180 °C).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2012/aamick.2012.4.issue-12/am301841p/production/images/medium/am-2012-01841p_0008.gif'></P>

Washing-Free Heterogeneous Immunosensor Using Proximity-Dependent Electron Mediation between an Enzyme Label and an Electrode

Dutta, Gorachand,Kim, Sinyoung,Park, Seonhwa,Yang, Haesik American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.9

<P>Washing processes, essential in most heterogeneous labeled assays, have been a big hurdle in simplifying the detection procedure and reducing assay time. Nevertheless, less attention has been paid to washing-free heterogeneous labeled assays. We report a purely washing-free immunosensor that allows fast, sensitive, and single-step detection of prostate-specific antigen in serum with low interference. Proximity-dependent electron mediation of ferrocenemethanol (Fc) between an indium–tin oxide (ITO) electrode and a glucose-oxidase (GOx) label allows us to discriminate between a bound and an unbound label: a bound label offers faster electron mediation than an unbound one. The electrooxidation of Fc at a low applied potential (0.13 V vs Ag/AgCl) and a low electrocatalytic ITO electrode and the oxidation of <SMALL>l</SMALL>-ascorbic acid by <SMALL>l</SMALL>-ascorbate oxidase minimize the effect of the interfering species. With a high concentration of glucose (200 mM), the signal and background levels are hardly dependent on the glucose-concentration variation in the sample. The washing-free immunosensor can detect a concentration of ca. 1 pg/mL for mouse IgG in phosphate-buffered saline and a concentration of ca. 10 pg/mL for prostate-specific antigen spiked in female serum after an incubation period of 10 min. The concentrations measured with actual clinical serum samples are in good agreement with the concentrations measured with a commercial instrument, which renders the washing-free heterogeneous immunosensor appealing for practical use.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-9/ac5006487/production/images/medium/ac-2014-006487_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac5006487'>ACS Electronic Supporting Info</A></P>

Low-Interference Washing-Free Electrochemical Immunosensor Using Glycerol-3-phosphate Dehydrogenase as an Enzyme Label

Dutta, Gorachand,Park, Seonhwa,Singh, Amardeep,Seo, Jeongwook,Kim, Sinyoung,Yang, Haesik American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.7

<P>In washing-free electrochemical detection, various redox and reactive species cause significant interference. To minimize this interference, we report a washing-free electrochemical immunosensor using flavin adenine dinucleotide (FAD)-dependent glycerol-3-phosphate dehydrogenase (GPDH) and glycerol-3-phosphate (GP) as an enzyme label and its substrate, respectively, because the reaction of FAD-dependent dehydrogenases with dissolved O<SUB>2</SUB> is slow and the level of GP preexisting in blood is low (<0.1 mM). A combination of a low electrocatalytic indium–tin oxide (ITO) electrode and fast electron-mediating Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> is employed to obtain a high signal-to-background ratio via proximity-dependent electron mediation of Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> between the ITO electrode and the GPDH label. Electrochemical oxidation of GPDH-generated Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>2+</SUP> is performed at 0.05 V vs Ag/AgCl, at which point the electrochemical interference is very low. When a washing-free immunosensor is applied to cardiac troponin I detection in human serum, the calculated detection limit is approximately 10 pg/mL, indicating that the immunosensor is very sensitive in spite of the use of washing-free detection with a short detection period (10 min for incubation and 100 s for electrochemical measurement). The low-interference washing-free electrochemical immunosensor shows good promise for fast and simple point-of-care testing.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-7/ac504485a/production/images/medium/ac-2014-04485a_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac504485a'>ACS Electronic Supporting Info</A></P>

Evaluation of turbulence models in rough-wall boundary layers for hydroelectric applications

Dutta, Rabijit,Nicolle, Jonathan,Giroux, Anne-Marie,Piomelli, Ugo Korean Society for Fluid machinery 2017 International journal of fluid machinery and syste Vol.10 No.3

The accuracy of turbulence models for the Reynolds-Averaged Navier-Stokes (RANS) equations in rough-wall flows is evaluated using data from large-eddy simulations (LES) of boundary layers with favourable and adverse pressure gradients. Some features of the flow (such as flow reversal in the roughness sublayer) cannot be captured accurately by any model, due to the fundamental model formulation. In mild pressure gradients most RANS models are sufficiently accurate for engineering applications, but if strong favourable or adverse pressure gradients are applied (especially those leading to separation) the model performance rapidly degrades.

Bi-Event Subtraction Technique at hadron colliders

Dutta, B.,Kamon, T.,Kolev, N.,Krislock, A. North-Holland Pub. Co 2011 Physics letters: B Vol.703 No.4

We propose the Bi-Event Subtraction Technique (BEST) as a method of modeling and subtracting large portions of the combinatoric background during reconstruction of particle decay chains at hadron colliders. The combinatoric background arises when it is impossible to know experimentally which observed particles come from the decay chain of interest. The background shape can be modeled by combining observed particles from different collision events and be subtracted away, greatly reducing the overall background. This idea has been demonstrated in various experiments in the past. We generalize it by showing how to apply BEST multiple times in a row to fully reconstruct a cascade decay. We show the power of BEST with two simulated examples of its application towards reconstruction of the top quark and a supersymmetric decay chain at the Large Hadron Collider.