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Song, Donghui,Kim, Chansu,Park, Sung-Kee Elsevier science 2018 Information sciences Vol.447 No.-
<P><B>Abstract</B></P> <P>This paper presents a novel framework for high-level activity analysis based on late fusion using multi-independent temporal perception layers. The method allows us to handle temporal diversity of high-level activities. The framework consists of multi-temporal analysis, multi-temporal perception layers, and late fusion. We build two types of perception layers based on situation graph trees (SGT) and support vector machines (SVMs). The results obtained from the multi-temporal perception layers are fused into an activity score through a step of late fusion. To verify this approach, we apply the framework to violent events detection in visual surveillance and experiments are conducted by using three datasets: BEHAVE, NUS–HGA and some videos from YouTube that show real situations. We also compare the proposed framework with existing single-temporal frameworks. The experiments produced results with accuracy of 0.783 (SGT-based, BEHAVE), 0.702 (SVM-based, BEHAVE), 0.872 (SGT-based, NUS–HGA), and 0.699 (SGT-based, YouTube), thereby showing that using our multi-temporal approach has advantages over single-temporal methods.</P>
Song, Jiali,Li, Chao,Ye, Linglong,Koh, Changwoo,Cai, Yunhao,Wei, Donghui,Woo, Han Young,Sun, Yanming The Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.39
<P>A novel strategy involving judiciously fusing one thiophene/thieno[3,2-<I>b</I>]thiophene on only one side of an indacenodithiophene (IDT) unit to extend IDT backbone conjugation was developed, and three A-D-A type non-fullerene small molecules (TPT-2F, TPTT-2F, and TPTTT-2F) were designed and synthesized to investigate the influence of the extent of IDT core conjugation on their photovoltaic properties. Extending the IDT core conjugation could broaden absorption, upshift the lowest unoccupied molecular orbital (LUMO) energy level, enhance electron mobility, and increase intermolecular π-π stacking. When these three non-fullerene acceptors were applied in organic solar cells (OSCs), simultaneous enhancement of the open-circuit voltage (<I>V</I>oc), short-circuit current (<I>J</I>sc), and fill factor (FF) was obtained, with the degree of enhancement following the order TPT-2F < TPTT-2F < TPTTT-2F. As a result, the TPTTT-2F based OSCs yielded a high PCE of 12.03%. To the best of our knowledge, the PCE of 12.03% is among the highest values for asymmetric non-fullerene acceptor based OSCs so far. These results demonstrate that extending the conjugation of the IDT core is an effective approach to design highly efficient asymmetric non-fullerene acceptors.</P>
Reconstruction of Acetogenesis Pathway Using Short-Read Sequencing of Clostridium aceticum Genome.
Lee, Sooin,Song, Yoseb,Choe, Donghui,Cho, Suhyung,Yu, Seok Jong,Cho, Yongseong,Kim, Sun Chang,Cho, Byung-Kwan American Scientific Publishers 2015 Journal of nanoscience and nanotechnology Vol.15 No.5
<P>Clostridium aceticum is an anaerobic homoacetogen, able to reduce CO2 to multi-carbon products using the reductive acetyl-CoA pathway. This unique ability to use CO2 or CO makes the microbe a potential platform for the biotech industry. However, the development of genetically engineered homoacetogen for the large-scale production of commodity chemicals is hampered by the limited amount of their genetic and metabolic information. Here we exploited next-generation sequencing to reveal C. aceticum genome. The short-read sequencing produced 44,871,196 high quality reads with an average length of 248 bases. Following sequence trimming step, 30,256,976 reads were assembled into 12,563 contigs with 168-fold coverage and 1,971 bases in length using de Bruijn graph algorithm. Since the k-mer hash length in the algorithm is an important factor for the quality of output contigs, a window of k-mers (k-51 to k-201) was tested to obtain high quality contigs. In addition to the assembly metrics, the functional annotation of the contigs was investigated to select the k-mer optimum. Metabolic pathway mapping using the functional annotation identified the majority of central metabolic pathways, such as the glycolysis and TCA cycle. Further, these analyses elucidated the enzymes consisting of Wood-Ljungdahl pathway, in which CO2 is fixed into acetyl-CoA. Thus, the metabolic reconstruction based on the draft genome assembly provides a foundation for the functional genomics required to engineer C. aceticum.</P>
Kangsan KIM,Donghui CHOE,Yoseb SONG,Minjeong KANG,Seung-Goo LEE,Dae-Hee LEE,Byung-Kwan CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Bacteroides thetaiotaomicron represents a major symbiont of the human gut microbiome that is increasingly viewed as a promising candidate strain for microbial therapeutics. Here, we engineer B. thetaiotaomicron for heterologous production of non-native butyrate as a proof-of-concept biochemical at therapeutically relevant concentrations. Since B. thetaiotaomicron is not a natural producer of butyrate, we heterologously expressed a butyrate biosynthetic pathway in the strain, which led to the production of butyrate at the final concentration of 12 ㎎/L in a rich medium. Further optimization of butyrate production was achieved by a round of metabolic engineering guided by an expanded genome-scale metabolic model (GEM) of B. thetaiotaomicron. The in silico knock-out simulation of the expanded model showed that pta and ldhD were the potent knock-out targets to enhance butyrate production. The maximum titer and specific productivity of butyrate in the pta-ldhD double knockout mutant increased by nearly 3.4 and 4.8 folds, respectively. To our knowledge, this is the first engineering attempt that enabled butyrate production from a non-butyrate producing commensal B. thetaiotaomicron. The study also highlights that B. thetaiotaomicron can serve as an effective strain for live microbial therapeutics in human.
Bassiouny Saleh,Jinghua Jiang,Aibin Ma,Dan Song,Donghui Yang,Qiong Xu 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.7
The main objective of the present paper is to draw the attention of researchers towards the wear analysis of functionallygraded aluminum matrix composites (FGAMCs) reinforced with different types of particles. The quality of the productsand characteristics achieved by the functionally graded materials (FGMs) has been given increasing attention during recentdecades. FGMs provide a way to obtain gradients between two phases with variations of properties. The centrifuge castingprocess was used to produce continuous variation of graded materials. These continuous graded materials offer high strengthand enhanced wear resistances compared with traditional composite materials. This paper summarizes the effect of the variousreinforcement materials and wear test parameters on microstructure as well as wear properties in the FGAMCs obtainedthrough the centrifugal casting technique. The results found in the reviewed literature are classified according to types ofreinforcement particles (such as silicon carbide, alumina and boron carbide) and the influence of wear test parameters (suchas applied load, sliding distance, duration and sliding speed) on FGM composites. The main conclusions in this paper arederived from previous studies on experimental investigations on the wear characteristics of FGAMCs. The research gapsand future directions have been discussed which will be prolific to the researchers in the design and manufacture of FGMsproduction by centrifugal casting.
Bassiouny Saleh,Jinghua Jiang,Aibin Ma,Dan Song,Donghui Yang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.6
Mechanical characteristics of functionally graded materials (FGMs) can be optimized for specific application depending onthe demands of different engineering sectors using appropriate combinations of material amounts and processing parameters. In recent years, many approaches to the production of functionally graded metal matrix composites (FGMMCs) using thecentrifugal casting method have been proposed. This paper presents an overview of different available materials and processparameters for FGMs based on their mechanical and wear characteristics. The major conclusions in this paper are drawnfrom the previous studies on experimental investigations on mechanical properties and wear characteristics of FGMMCs. The findings in current work are classified according to material parameters such as the type of matrix material, reinforcingmaterial (type, weight, and size), in addition to process parameters such as mould rotational speed and pouring temperature.