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Mi-Young Chung,Chang Kil Kim,Chang Young Kim,Jeon Su Min,Do-JinLee,Aung Htay Naing,Jae Dong Chung 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.3
Tetraploids were successfully produced fromdiploid seeds obtained through interspecific crossingbetween Calanthe discolor and Calanthe sieboldii bytreating with colchicine or oryzalin. Colchicine was testedat concentrations of 0.05 and 0.1 % for 0, 3, or 7 days andoryzalin was tested at a concentration of 0.003 % for 1, 2,4, and 7 days, and the ploidy of the seedlings was determinedby flow cytometry. Tetraploids (49) were obtainedfrom the interspecific hybrid seeds treated with all colchicineand oryzalin concentrations. The most efficient conditionfor inducing tetraploids seemed to be treated with0.003 % oryzalin for 1 or 2 days. Cytological and morphologicalevidence confirmed the results of flow cytometricanalysis. The stomatal density and sizes of thetetraploid plants were significantly higher and larger thanthose of the diploid plants. Differences in leaf shape werefound between the tetraploid and diploid plants under thesame growing conditions: the leaves of the diploids wereelongated and those of the tetraploids were round.